FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991224
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACTGCCGTGTCGTCGGCCCTCAGGCTGTACATTTGCAGATAAA60100.60632107374317No Hit
GATTATTACTGTGGGTCATGGGATGACAGCCTGAATGGTTTCGTCTTCGGAAGTGCGACGAAGGTCACCGTCC30570.30840657611195854No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG28530.2878259606304932No Hit
GTACTAATCAGCGGCCCTCGGGGGTCCCTGACCGATTCTCCGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCA27800.28046132861996886No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTCGTCGCA27380.2762241430796672No Hit
GTATATTACTGTGCGAGAGATTGGAGGCCGACTGCGGTGACATATTATTT25830.2605869107285538No Hit
GATTAGTACTATAGATGAGGAGTTTGGGGGCCTTTCCTGGGACTTGCTGATACCAATAAACAATGTTGTATCCGA25680.25907363017844603No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG25600.2582665472183886No Hit
GTCCTGGGCCCAGTCTATGCTGACTCAGCCACCCTCAGTATCTGGGACCCCCGGGCAGAGGGTCACCATCTCT24560.24777446873764153No Hit
GTACTATAGATGAGGAGTTTGGGGGCCTTTCCTGGGACTTGCTGATACCA24550.24767358336763434No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC24360.24575676133749788No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG23800.24010718061709563No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC22250.22446994826598224No Hit
CTATAGTACTAATCAGCGGCCCTCGGGGGTCCCTGACCGATTCTCCGGCTCCAAGTCTGGCACCTCAGCCTCC21490.21680266014543634No Hit
GTAATAATCAGCCTCATCTTCAGACTGGAGACCACTGATGGCCAGGGAGG21250.21438141126526394No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTCGTCGCACTTCCGAAGACGAAACCATTCAGGCTGTCATCCCATGAC20960.21145573553505567No Hit
GTGCAGGGTCCTGGGCCCAGTCTATGCTGACTCAGCCACCCTCAGTATCTGGGACCCCCGGGCAGAGGGTCACCA20200.20378844741450974No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTCGTC18070.18229986360297976No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG17960.18119012453290073No Hit
GTCTATGCTGACTCAGCCACCCTCAGTATCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGGAGGCGG17790.17947507324277862No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17650.17806267806267806No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT17580.17735648047262778No Hit
CTCCTGTGCAGCGTCTGGATTCAGTTTCAGTAGTTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG17260.17412814863239792No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17230.1738254925223764No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16770.16918476550204595No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15800.15939888461134918No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT15730.1586926870212989No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC15570.15707852110118398No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT15400.15536346981106186No Hit
GTATATTACTGTGCGAGAGATTGGAGGCCGACTGCGGTGACATATTATTTCGGAATGGACGTCTGGGGCCAAGGG15360.15495992833103314No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15050.15183248186081047No Hit
GTATCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGGAGGCGGCTCTAACATCGGATACAACATTGTT14910.15042008668070989No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG14900.15031920131070273No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTATGCTGACTCAGCCAC14790.14920946224062373No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14780.14910857687061654No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14750.14880592076059498No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG14620.14749441095050161No Hit
CAGTAATAATCAGCCTCATCTTCAGACTGGAGACCACTGATGGCCAGGGA14030.1415421741200778No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13970.1409368619000347No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13900.14023066430998443No Hit
GGGCTGGAGTGGGTGGCGTTCACATGGTATGATGGAAGTAGAAAATATTA13890.14012977893997725No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA13200.13316868840948162No Hit
ACCCAGGACGGTGACCTTCGTCGCACTTCCGAAGACGAAACCATTCAGGC13050.13165540785937388No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC13040.1315545224893667No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12960.13074743952930923No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC12960.13074743952930923No Hit
CAGTAATATACTGCCGTGTCGTCGGCCCTCAGGCTGTACATTTGCAGATAAAGCGTGTCGTTGGAATTGTCTC12900.1301421273092661No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA12840.12953681508922302No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC12680.1279226491691081No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12470.12580405639895725No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC12400.12509785880890698No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12180.12287838066874894No Hit
TTATTACTGTGGGTCATGGGATGACAGCCTGAATGGTTTCGTCTTCGGAAGTGCGACGAAGGTCACCGTCCTGGG11910.120154475678555No Hit
ATATTACTGTGCGAGAGATTGGAGGCCGACTGCGGTGACATATTATTTCGGAATGGACGTCTGGGGCCAAGGGAC11880.11985181956853344No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11850.1195491634585119No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGAAGAGGAGCCCAGCACTAGAAG11700.11803588290840415No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11560.11662348772830358No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG11380.1148075510681743No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11320.11420223884813119No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC11270.11369781199809528No Hit
GTATGATGGAAGTAGAAAATATTATGCAGATTCTGTGAGGGGCCGATTCAGCATCTCCAGAGACAATTCCAACGA11250.11349604125808091No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT11220.11319338514805936No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC11110.11208364607798035No Hit
CTCTTGCTCTGGAGGCGGCTCTAACATCGGATACAACATTGTTTATTGGTATCAGCAAGTCCCAGGAAAGGCCCC11050.11147833385793725No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11050.11147833385793725No Hit
GGCCATCAGTGGTCTCCAGTCTGAAGATGAGGCTGATTATTACTGTGGGTCATGGGATGACAGCCTGAATGGTT11030.11127656311792289No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10620.10714026294762838No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGTCTCCAGTCTGAAGATGAGGCTGATTATTACTGTGGGTCA10580.10673672146759966No Hit
GTCATCCCATGACCCACAGTAATAATCAGCCTCATCTTCAGACTGGAGACCACTGATGGCCAGGGAGGCTGAGG10560.1065349507275853No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10520.10613140924755657No Hit
GTTTATTGGTATCAGCAAGTCCCAGGAAAGGCCCCCAAACTCCTCATCTA10480.10572786776752784No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10470.10562698239752065No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10450.10542521165750629No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10380.104719014067456No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10300.10391193110739853No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG10280.10371016036738417No Hit
GACCCAGGACGGTGACCTTCGTCGCACTTCCGAAGACGAAACCATTCAGG10140.1022977651872836No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAACTAC10020.10108714074719741No Hit
AGTCTATGCTGACTCAGCCACCCTCAGTATCTGGGACCCCCGGGCAGAGG10000.10088537000718305No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCGGAGAATCGGTC9980.10068359926716867No Hit
GAGTACGGGAGCTCTGGAAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACA9960.10048182852715432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)