FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991224
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60940.6147954448237734No Hit
GTAATATACTGCCGTGTCGTCGGCCCTCAGGCTGTACATTTGCAGATAAAGCGTGTCGTTGGAATTGTCTCTGGA54070.5454871956288387No Hit
GTATATTACTGTGCGAGAGATTGGAGGCCGACTGCGGTGACATATTATTTCGGAATGGACGTCTGGGGCCAAGG39620.3997078359684592No Hit
GATTATTACTGTGGGTCATGGGATGACAGCCTGAATGGTTTCGTCTTCGGAAGTGCGACGAAGGTCACCGTCCTG28350.2860100239703639No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT26380.26613560607894887No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC25580.2580647764783742No Hit
GTACTATAGATGAGGAGTTTGGGGGCCTTTCCTGGGACTTGCTGATACCAATAAACAATGTTGTATCCGATGTTA24480.24696738577758406No Hit
GTACTAATCAGCGGCCCTCGGGGGTCCCTGACCGATTCTCCGGCTCCAAG24260.24474790763742604No Hit
GTCCTGGGCCCAGTCTATGCTGACTCAGCCACCCTCAGTATCTGGGACCCCCGGGCAGAGGGTCACCATCTCTT24050.2426293148672752No Hit
GATTAGTACTATAGATGAGGAGTTTGGGGGCCTTTCCTGGGACTTGCTGATACCAATAAACAATGTTGTATCC23050.23254077786655689No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTCGTCGCA23020.23223812175653535No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT22240.22436906289597505No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC21730.21922390902560873No Hit
CTATAGTACTAATCAGCGGCCCTCGGGGGTCCCTGACCGATTCTCCGGCTCCAAGTCTGGCACCTCAGCCTCC20600.20782386221479707No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG20160.20338490593448103No Hit
GTAATAATCAGCCTCATCTTCAGACTGGAGACCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCGGA18730.18895829802345385No Hit
GTGCAGGGTCCTGGGCCCAGTCTATGCTGACTCAGCCACCCTCAGTATCT18550.18714236136332454No Hit
GTCTATGCTGACTCAGCCACCCTCAGTATCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGGAGGCG18530.18694059062331017No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTCGTCGCACTTCCGAAGACGAAACCATTCAGGCTGTCATCCCATGAC18360.18522553933318806No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCA18290.18451934174313778No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTCGTCGCACTTCCGAAGACGAAACCATTCA16590.16736882884191667No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAACTACTGAAACTGAATCCAGACGCTGCACA16350.16494757996174425No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTATGCTGACTCAGCCACCCTCAGTATCTGGGACCCCCGGGCA16230.16373695552165807No Hit
CTCCTGTGCAGCGTCTGGATTCAGTTTCAGTAGTTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGG16000.16141659201149286No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16000.16141659201149286No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG15770.15909622850132765No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC15250.15385018926095412No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA14970.151025398900753No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14370.14497227670032203No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14120.14245014245014245No Hit
GGGCTGGAGTGGGTGGCGTTCACATGGTATGATGGAAGTAGAAAATATTA13840.13962535208994134No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG13810.13932269597991978No Hit
GTATCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGGAGGCGG13790.1391209252399054No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13780.13902003986989822No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC13690.1381120715398336No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13620.1374058739497833No Hit
GTATGATGGAAGTAGAAAATATTATGCAGATTCTGTGAGGGGCCGATTCAGCATCTCCAGAGACAATTCCAAC13610.13730498857977613No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13320.1343793128495678No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT13260.13377400062952471No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT13140.1325633761894385No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT12940.13054566878929486No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC12860.1297385858292374No Hit
TTATTACTGTGGGTCATGGGATGACAGCCTGAATGGTTTCGTCTTCGGAA12630.12741822231907218No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC12550.12661113935901472No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG12450.12560228565894288No Hit
ACCCAGGACGGTGACCTTCGTCGCACTTCCGAAGACGAAACCATTCAGGC12290.12398811973882795No Hit
CAGTAATATACTGCCGTGTCGTCGGCCCTCAGGCTGTACATTTGCAGATAAAGCGTGTCGTTGGAATTGTCTCTG12210.12318103677877049No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA11950.12055801715858373No Hit
CTCTTGCTCTGGAGGCGGCTCTAACATCGGATACAACATTGTTTATTGGTATCAGCAAGTCCCAGGAAAGGCCCC11700.11803588290840415No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC11650.11753145605836823No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG11640.11743057068836105No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11580.11682525846831796No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11530.11632083161828204No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT11460.11561463402823176No Hit
CAGTAATAATCAGCCTCATCTTCAGACTGGAGACCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCG11430.11531197791821021No Hit
ATATTACTGTGCGAGAGATTGGAGGCCGACTGCGGTGACATATTATTTCGGAATGGACGTCTGGGGCCAAGGGAC11390.11490843643818148No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG11390.11490843643818148No Hit
GGCCATCAGTGGTCTCCAGTCTGAAGATGAGGCTGATTATTACTGTGGGTCATGGGATGACAGCCTGAATGGTTT10960.1105703655278726No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAG10910.11006593867783669No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10870.10966239719780796No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCT10500.1059296385075422No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10420.10512255554748473No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGTCTCCAGTCTGAAGATGAGGCTGATTATTACTGTGGGTCATG10330.1042145872174201No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10200.10290307740732671No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT10080.10169245296724051No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC10060.10149068222722614No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGAAGAGGAGCCCAGCACTAGAAG10030.1011880261172046No Hit
GTTTATTGGTATCAGCAAGTCCCAGGAAAGGCCCCCAAACTCCTCATCTATAGTACTAATCAGCGGCCCTCGGGG10010.10098625537719023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)