FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621486
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCGTCGACTCTCAGACTGTTCATTTCCAGATAAA24680.39711272659400215No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21150.34031337793610794No Hit
GTCTATTACTGTGTAAGTTCCGGGAGATGGCTCCAATTAGACAACTGGGGCCAGGGAACCCTGGTCACCGTCTC20210.3251883389167254No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT19860.3195566754520617No Hit
GAGTTACAGTCCCCTCAGTTTCGGCGGTTCCCTCACTTTCGGCGGAGGGACCACGGTGGACATCAAACGAACTG15330.24666685975227118No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15270.2457014317297574No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT14660.2358862468342006No Hit
GATCAGGACTGCACACAGCGAACTCACCATGGAATTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGG14180.22816282265409035No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13770.22156573116691286No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13630.21931306578104737No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG13420.2159340677022491No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12660.2037053127504079No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12620.20306169406873203No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12450.20032631467160966No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12080.194372841866108No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT11060.17796056548337372No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10950.17619061410876513No Hit
CCATTTACAGTCTGGAACCTGAAGATTTTGCAACTTATTACTGTCAACAG10880.17506428141583238No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC10800.17377704405248068No Hit
GTCTGAGAGTCGACGACACGGCTGTCTATTACTGTGTAAGTTCCGGGAGA10710.17232890201870998No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT10640.17120256932577724No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10570.1700762366328445No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAGTTACTGGATGCACTGGG10470.16846718992865486No Hit
GGACTGCACACAGCGAACTCACCATGGAATTTGGGCTGAGCTGGGTTTTC9610.154629388272624No Hit
CTGTAACTCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTCCAGACTGTAAATGGTGAGAGTGAAATCTGTC9550.15366396025011023No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGCGCAAGTCAGAATGTTAACAACTATTTAAATTGGTATCAACAAAAA9520.15318124623885332No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9310.1498022481600551No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9270.14915862947837924No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9090.14626234541083788No Hit
GATCAGGAGTTGAGGGACTTTTCCTGGTTTTTGTTGATACCAATTTAAAT9040.14545782205874308No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8770.14111339595743105No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8730.14046977727575521No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8540.1374125885377949No Hit
GTCTCCATCCTCCCTGTCTGCCTCTGTAGGAGACAGAGTCACCATCACTTGCCGCGCAAGTCAGAATGTTAACAA8480.13644716051528114No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8460.1361253511744432No Hit
GATTTCACTCTCACCATTTACAGTCTGGAACCTGAAGATTTTGCAACTTATTACTGTCAACAGAGTTACAGTCCC8370.13467720914067252No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8370.13467720914067252No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC8360.13451630447025356No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8290.1333899717773208No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG8280.13322906710690183No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA8260.1329072577660639No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG8050.12952825968726567No Hit
GTAAATGGTGAGAGTGAAATCTGTCCCAGCCCCACTGCCACTGAACCTTG8030.12920645034642775No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7940.12775830831265708No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCGTGGTCC7830.1259883569380485No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCGTGGTCCCTCCGCCGAAAGTGAGGGAACCGCCGAAACTGAGGGGA7760.12486202424511575No Hit
CTGTAAATGGTGAGAGTGAAATCTGTCCCAGCCCCACTGCCACTGAACCTTGATGGGACCCCACTCTGCAAAC7750.12470111957469678No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG7670.12341388221134507No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7550.1214830261663175No Hit
GTACGCGGACTCCGTGAAGGGTCGATTTACCATCTTCAGAGACAACGTCAGGAACACAGTTTATCTGGAAATGAA7460.12003488413254683No Hit
ACTTATTACTGTCAACAGAGTTACAGTCCCCTCAGTTTCGGCGGTTCCCTCACTTTCGGCGGAGGGACCACGG7460.12003488413254683No Hit
CTCTCAGACTGTTCATTTCCAGATAAACTGTGTTCCTGACGTTGTCTCTG7400.11906945611003304No Hit
GTCTGGAACCTGAAGATTTTGCAACTTATTACTGTCAACAGAGTTACAGTCCCCTCAGTTTCGGCGGTTCCCTCA7380.11874764676919512No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7380.11874764676919512No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7370.11858674209877615No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7180.11552955336081586No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGCTGACCCAGTC7180.11552955336081586No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACA7160.11520774401997792No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7020.11295507863411244No Hit
GCATAGATCAGGAGTTGAGGGACTTTTCCTGGTTTTTGTTGATACCAATTTAAATAGTTGTTAACATTCTGACT6970.11215055528201762No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA6960.11198965061159866No Hit
ATCACTAAGTACGCGGACTCCGTGAAGGGTCGATTTACCATCTTCAGAGACAACGTCAGGAACACAGTTTATC6960.11198965061159866No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6950.1118287459411797No Hit
CACCATTTACAGTCTGGAACCTGAAGATTTTGCAACTTATTACTGTCAACAGAGTTACAGTCCCCTCAGTTTCGG6940.11166784127076074No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT6930.11150693660034176No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG6930.11150693660034176No Hit
GCCCTGGGATTCCCAGCTGTTTCTGCGTGCTGATCAGGACTGCACACAGC6910.11118512725950384No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6910.11118512725950384No Hit
GATCTATGCTGCCTCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGGCTGGGACAGATTTCA6840.1100587945665711No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6810.1095760805553142No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6800.10941517588489524No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG6790.10925427121447628No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG6730.10828884319196248No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6710.10796703385112455No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC6650.10700160582861078No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGCGCAAGTCAGAATGTTAAC6630.10667979648777284No Hit
GAGTGAAATCTGTCCCAGCCCCACTGCCACTGAACCTTGATGGGACCCCACTCTGCAAACTGGAGGCAGCATAGA6610.10635798714693492No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC6590.10603617780609699No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG6520.10490984511316426No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTGTCTAATTGGAGCCA6430.10346170307939358No Hit
GTTACAGTCCCCTCAGTTTCGGCGGTTCCCTCACTTTCGGCGGAGGGACCACGGTGGACATCAAACGAACTG6410.10313989373855566No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6280.10104813302310914No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT6220.10008270500059535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)