FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621486
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCGTCGACTCTCAGACTGTTCATTTCCAGATAAACTGTGTTCCTGACGTTGTCTCTGAA22410.3605873664088974No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA20250.3258319575984013No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18160.29220288148083784No Hit
GTCTATTACTGTGTAAGTTCCGGGAGATGGCTCCAATTAGACAACTGGGGCCAGGGAACCCTGGTCACCGTCTC17270.27788236581355014No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16820.2706416556446968No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15240.2452187177185005No Hit
GAGTTACAGTCCCCTCAGTTTCGGCGGTTCCCTCACTTTCGGCGGAGGGACCACGGTGGACATCAAACGAACTGT14660.2358862468342006No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC14530.23379448611875409No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13560.21818673308811465No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT12950.20837154819255782No Hit
GATCAGGACTGCACACAGCGAACTCACCATGGAATTTGGGCTGAGCTGGG12740.2049925501137596No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11890.1913156531281477No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA11840.1905111297760529No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10950.17619061410876513No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA10770.1732943300412238No Hit
GTCTGAGAGTCGACGACACGGCTGTCTATTACTGTGTAAGTTCCGGGAGA10570.1700762366328445No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10330.16621452454278937No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGCGCAAGTCAGAATGTTAACAACTATTTAAATTGGTATCAACAAAAA10000.16090467041896359No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC9850.1584911003626791No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9540.15350305557969127No Hit
CCATTTACAGTCTGGAACCTGAAGATTTTGCAACTTATTACTGTCAACAGAGTTACAGTCCCCTCAGTTTCGGCG9500.1528594368980154No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC9380.15092858085298785No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAGTTACTGGATGCACTGGGTCCGCCAAGTTTCAGGGAAGGCACC9360.1506067715121499No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA9270.14915862947837924No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA9220.14835410612628444No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9210.14819320145586545No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8970.14433148936581033No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG8820.14191791930952588No Hit
GTCTCCATCCTCCCTGTCTGCCTCTGTAGGAGACAGAGTCACCATCACTTGCCGCGCAAGTCAGAATGTTAACAA8580.13805620721947076No Hit
GGACTGCACACAGCGAACTCACCATGGAATTTGGGCTGAGCTGGGTTTTC8560.13773439787863284No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC8530.13725168386737593No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8500.13676896985611905No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG8340.13419449512941561No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8280.13322906710690183No Hit
CTGTAACTCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTCCAGACTGTAAATGGTGAGAGTGAAATCTGTC8250.13274635309564495No Hit
GATCAGGAGTTGAGGGACTTTTCCTGGTTTTTGTTGATACCAATTTAAATAGTTGTTAACATTCTGACTTGCGCG8100.1303327830393605No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGT7920.12743649897181916No Hit
CTCCTGATCTATGCTGCCTCCAGTTTGCAGAGTGGGGTCCCATCAAGGTT7830.1259883569380485No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7770.1250229289155347No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7660.12325297754092611No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG7660.12325297754092611No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7640.12293116820008819No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT7630.12277026352966922No Hit
GATTTCACTCTCACCATTTACAGTCTGGAACCTGAAGATTTTGCAACTTATTACTGTCAACAGAGTTACAGTCCC7530.12116121682547956No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGCTGACCCAGTCTCCATCCTCCCTGTCTGCCTCTGTA7460.12003488413254683No Hit
GATCTATGCTGCCTCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGGCTGGGACAGATTTCA7380.11874764676919512No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGCGCAAGTCAGAATGTTAAC7230.11633407671291067No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT7170.11536864869039688No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7090.11408141132704518No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7080.11392050665662622No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCGTGGTCCCTCCGCCGAAAGTGAGGGAACCGCCGAAACTGAGGG7070.11375960198620726No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7030.1131159833045314No Hit
CTCTCAGACTGTTCATTTCCAGATAAACTGTGTTCCTGACGTTGTCTCTGAAGATGGTAAATCGACCCTTCACG7000.11263326929327451No Hit
ACTTATTACTGTCAACAGAGTTACAGTCCCCTCAGTTTCGGCGGTTCCCTCACTTTCGGCGGAGGGACCACGGTG6930.11150693660034176No Hit
GTCTGGAACCTGAAGATTTTGCAACTTATTACTGTCAACAGAGTTACAGTCCCCTCAGTTTCGGCGGTTCCCTCA6930.11150693660034176No Hit
CACCATTTACAGTCTGGAACCTGAAGATTTTGCAACTTATTACTGTCAACAGAGTTACAGTCCCCTCAGTTTCG6920.1113460319299228No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6810.1095760805553142No Hit
CTGTAAATGGTGAGAGTGAAATCTGTCCCAGCCCCACTGCCACTGAACCTTGATGGGACCCCACTCTGCAAACTG6780.10909336654405732No Hit
ATCACTAAGTACGCGGACTCCGTGAAGGGTCGATTTACCATCTTCAGAGACAACGTCAGGAACACAGTTTATCTG6680.10748431983986767No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6620.10651889181735388No Hit
GTACGCGGACTCCGTGAAGGGTCGATTTACCATCTTCAGAGACAACGTCAGGAACACAGTTTATCTGGAAATGAA6600.10619708247651596No Hit
GTAAATGGTGAGAGTGAAATCTGTCCCAGCCCCACTGCCACTGAACCTTGATGGGACCCCACTCTGCAAACTGGA6600.10619708247651596No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT6590.10603617780609699No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC6530.10507074978358322No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT6510.10474894044274528No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6460.10394441709065047No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6440.10362260774981255No Hit
GAGTGAAATCTGTCCCAGCCCCACTGCCACTGAACCTTGATGGGACCCCACTCTGCAAACTGGAGGCAGCATA6440.10362260774981255No Hit
GCATAGATCAGGAGTTGAGGGACTTTTCCTGGTTTTTGTTGATACCAATTTAAATAGTTGTTAACATTCTGACTT6390.10281808439771772No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCGTGGTCCCTCCGCCGAAAGTGAGGGAACCGCC6220.10008270500059535No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC6220.10008270500059535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)