FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597158
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTGAACTCCATGTAGGCTGTGCTTATGGAGCTGTTCCTGG21560.3610434759309931No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13900.23276921685718016No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT11880.1989423234721799No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10950.18336855572562036No Hit
GGTTATACCACTCCAAATATCGTAATGGGGCCGGCCTCTCGCACAGTAAT9910.16595272942839248No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTGAACTCCATGTAGGCTGTGCTTATGGAGCTGTTCC9860.1651154300871796No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9690.1622686123270558No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9690.1622686123270558No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9020.15104880115480324No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT8920.1493742024723775No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG8610.14418294655685765No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8550.14317818734740217No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT8250.13815439130012494No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8240.13798693143188234No Hit
AGGTTATACCACTCCAAATATCGTAATGGGGCCGGCCTCTCGCACAGTAA8230.13781947156363977No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8060.134972653803516No Hit
CCATGTAGGCTGTGCTTATGGAGCTGTTCCTGGTCATGGTGACTCTGCCCTGGAACTTCTGTGCATAGCCTGTG8030.13447027419878826No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG8020.1343028143305457No Hit
CCCTTGGACAGTCGGCCTCCATCTCCTGCAGGTCTAGCCAAAGCCTCGTA8020.1343028143305457No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7910.13246075577987734No Hit
GTGTATTACTGTGCGAGAGGCCGGCCCCATTACGATATTTGGAGTGGTAT7610.12743695973260008No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC7610.12743695973260008No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7470.12509252157720402No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7410.12408776236774857No Hit
CTCCCAGGTGCAGCTGGTTCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGG7360.1232504630265357No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7200.12057110513465448No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGCGACAGTCGGGGG7170.12006872552992676No Hit
GTATAACCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG7070.118394126847501No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA7010.11738936763804554No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGG6940.11621714856034752No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6810.11404017027319402No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGTCGATTTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA6750.11303541106373859No Hit
GTTTATTACTGCATGCAGGCTACACAATTTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA6540.10951875383064448No Hit
GTATTACTGTGCGAGAGGCCGGCCCCATTACGATATTTGGAGTGGTATAACCTGGGGCCAGGGAACCCTGGTCAC6430.10767669527997614No Hit
GGTATAACCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCC6330.1060020965975504No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGG6290.10533225712458008No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC6280.10516479725633752No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6160.1031552788374266No Hit
GGCTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGAACAGCTC6140.10282035910094146No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGTTATACCACTCCAAATA6110.10231797949621373No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGG6070.10164814002324343No Hit
CATCTGTCCTCTAGAGAAAACCCTGTGAGCACAGCTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGG6030.10097830055027313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTAGA3850.049.7439770
GTATCAA12400.040.208091
AATGGTA2558.731149E-1139.52823370
CGTATGC1900.037.9578241
GGTATCA4650.037.9287221
AAAACGG400.001632762837.91567667
AAACGGG450.00165087637.8297768
GTATGCC2200.037.4998542
TCGTATG2000.036.02326640
CTTATAC2200.035.4587141
CCCACGA2300.035.3827919
ATGCCGT2200.034.43039744
GGCAATC1900.034.0682833
CGAGACA2350.033.19817423
ATCAACG15100.032.7802243
CTTGAAA2250.032.65428557
AATCTCG1900.032.3596236
TCAACGC15400.032.1416444
AACGCAG15450.032.0376246
AGGCAAT2450.031.96632832
Produced by FastQC (version 0.11.5)