FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597158
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTGAACTCCATGTAGGCTGTGCTTATGGAGCTGTTCCTGGT20630.3454697081844336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19770.3310681595155721No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12380.2073153168843087No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA12120.2029613603100017No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10280.1721487445533678No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTGAACTCCATGTAGGCTGTGCTTATGGAGCTGTTCCTG9230.15456545838789734No Hit
GGTTATACCACTCCAAATATCGTAATGGGGCCGGCCTCTCGCACAGTAATACACGGCCGTGTCCTCAGATCTCAG9140.15305831957371416No Hit
AGGTTATACCACTCCAAATATCGTAATGGGGCCGGCCTCTCGCACAGTAATACACGGCCGTGTCCTCAGATCTCA8770.14686230444873888No Hit
CCATGTAGGCTGTGCTTATGGAGCTGTTCCTGGTCATGGTGACTCTGCCCTGGAACTTCTGTGCATAGCCTGTGT8690.14552262550279824No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8360.13999644985079324No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA8350.1398289899825507No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC7970.13346551498933282No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC7950.13313059525284765No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG7930.1327956755163625No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7750.12978139788799614No Hit
GTGTATTACTGTGCGAGAGGCCGGCCCCATTACGATATTTGGAGTGGTAT7660.12827425907381296No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA7420.12425522223599114No Hit
CCCTTGGACAGTCGGCCTCCATCTCCTGCAGGTCTAGCCAAAGCCTCGTACACACTGATGGAAACACCTACTTGA7400.123920302499506No Hit
GGTATAACCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCT7380.12358538276302083No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT7250.12140840447586736No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGTCGATTTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA7010.11738936763804554No Hit
CTCCCAGGTGCAGCTGGTTCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAG6750.11303541106373859No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC6730.11270049132725342No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGTTATACCACTCCAAATAT6630.11102589264482766No Hit
GTTTATTACTGCATGCAGGCTACACAATTTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA6620.11085843277658508No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC6530.10935129396240191No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6470.10834653475294646No Hit
GTATTACTGTGCGAGAGGCCGGCCCCATTACGATATTTGGAGTGGTATAACCTGGGGCCAGGGAACCCTGGTCAC6270.10499733738809494No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT6260.10482987751985237No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG6160.1031552788374266No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6150.10298781896918403No Hit
GGCTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGAACAGCTC6070.10164814002324343No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6070.10164814002324343No Hit
GTATAACCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGA6050.10131322028675827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCTA1301.2862256E-767.67110470
TACGCTG150.002563565567.53886410
AAGCGGT4800.051.92817770
TGAGCTT1552.8860564E-547.2970170
AATGGTA2301.2835153E-744.62370370
GTATCAA14200.038.5644451
GAGTTCA7900.037.1191770
AAAGTCA1600.001922695536.6551870
GAGCAAA9500.033.95427370
ATCAACG16300.032.9352153
GGTATCA5800.032.6377681
TCAACGC16350.032.42424
CAACGCA16450.032.021825
CACCAGT7800.031.955870
AACGCAG16850.031.2616636
GAAAGTT1900.00446383330.86752170
AAATTGT4751.2350938E-930.86752170
TCCTGGT8350.030.66957369
CAGCCAT8150.030.58346470
AATAGTC450.00487141630.33741436
Produced by FastQC (version 0.11.5)