FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218097
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG55780.4579274064380751No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG35940.2950503941804306No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34720.2850347714508779No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACATAAGTTTTACTACCTGCATATGAA32030.26295114428489685No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT27900.22904579848731257No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACATAAGTTTTA27610.22666503570733693No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCACCATCTCC25050.2056486470289312No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24500.20113340727380496No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24470.2008871214689799No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24410.20039454985932978No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24240.19899893029865437No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23680.19440159527525314No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC23240.1907894034711521No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22360.18356501986295015No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCCGTGATCTT22340.18340082932640012No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21170.1737956829382225No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC20140.16533987030589517No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG19780.16238444064799437No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19650.1613172021604191No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19350.15885434411216842No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19220.1577871056245931No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG18590.1526151037232667No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18380.15089110308949122No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCAC18360.15072691255294118No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18280.15007015040674102No Hit
CTCCTGCACTGGAACCAGCCGTGATCTTGGGAGTTACAATCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC17570.14424138635921444No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCCGTGATCTTGGGAGTTACAATCTTGTCTCCTGGTACCAACAACA17500.14366671948128926No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16800.13792005070203767No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16800.13792005070203767No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16790.13783795543376268No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGG16690.13701700275101245No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16690.13701700275101245No Hit
CCCCTGAGGGCCGCTTAATGTCATCCTTAATCAAGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA16600.13627814533653723No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16390.13455414470276175No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT16390.13455414470276175No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG16000.13135242924003587No Hit
CCACATAAGTTTTACTACCTGCATATGAACAACAATAATAAGTGGCCTCGTCTTCAGCCTGGAGCCCGGAAATTG15920.1306956670938357No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT15890.13044938128901065No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15880.1303672860207356No Hit
GACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCCGTGATCT15640.12839699958213507No Hit
GTGTCTGGTGGCTCCACCAGTTATTACTTTTGGAGTTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGAT15430.1266729989483596No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15090.12388175982700886No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG15040.12347128348563373No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14660.12035166329118288No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT14300.11739623363328207No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13850.11370194656090606No Hit
CCTTGGTCCCTCCGCCGAACACCACATAAGTTTTACTACCTGCATATGAACAACAATAATAAGTGGCCTCGTCT13820.11345566075608099No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG13630.11189585065885559No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13360.10967927841542997No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT13330.1094329926106049No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13210.10844784939130464No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACATAAGTTT13100.10754480144027939No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA13080.10738061090372934No Hit
GCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCCG13040.10705222983062926No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACT13010.10680594402580419No Hit
CCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATTTCCGGGCTCCAGG12830.10532822919685379No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12790.1049998481237537No Hit
GGATGACATTAAGCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCA12740.10458937178237859No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACATAAGTTTTAC12600.10344003802652826No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA12540.10294746641687812No Hit
CTTATTATTGTTGTTCATATGCAGGTAGTAAAACTTATGTGGTGTTCGGC12380.10163394212447778No Hit
ACATTAAGCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATT12290.10089508471000258No Hit
GCTTAATGTCATCCTTAATCAAGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGATTGTAA12260.10064879890517751No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12200.10015622729552737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3900.080.5130470
CGTATGC3550.045.39594341
GGTATCA11850.044.865241
TCGTATG3700.044.43794340
ATATCGT405.3221112E-542.6742437
ATCTCGT3050.042.53432537
GTGGTAT2650.042.1693341
GGCAATC3100.041.70844333
TATCGTA504.1037947E-641.00631338
GCCGTCT3800.040.87320346
ATGCCGT3850.040.21951344
GTATGCC4100.040.19267742
TATACAC4250.039.8358463
ACGAGAC4100.038.82203722
CTTATAC4300.038.5883561
CCCACGA4050.038.45406719
CCGTCTT4100.037.9205747
AATATCG451.0707219E-437.90128336
TTATACA4300.037.7961852
GCAATCT3450.037.50500534
Produced by FastQC (version 0.11.5)