FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218097
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT53010.4351870171258939No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37310.3062974459341087No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37280.30605116012928363No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33940.27863134052542615No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACATAAGTTTTACTACCTGCATATGAA29120.23906142121686536No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA26450.2171419845874343No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25260.20737264766270666No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24610.20203645522483019No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23760.1950583574214533No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23600.19374483312905294No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA23180.19029683186150198No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCACCATCTCCT23050.18922959337392672No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG22860.18766978327670128No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG22200.1822514955705498No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCACATAAGTTTTA21850.179378161180924No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCCGTGATCTT21750.17855720849817378No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC21660.1778183510836986No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA20350.16706387093967065No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCAC20310.16673548986657058No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19080.1566377718687428No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG18080.14842824504124058No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT17920.1471147207488402No Hit
CTCCTGCACTGGAACCAGCCGTGATCTTGGGAGTTACAATCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC17850.14654005387091504No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG17570.14424138635921444No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG17460.14333833840818916No Hit
CCCCTGAGGGCCGCTTAATGTCATCCTTAATCAAGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGA17280.14186062357923876No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17200.1412038614330386No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16930.13898728918961298No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT16820.13808424123858773No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16140.1325017629958862No Hit
CCACATAAGTTTTACTACCTGCATATGAACAACAATAATAAGTGGCCTCG16010.13143452450831092No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15860.13020309548418557No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC15750.12930004753316035No Hit
GACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCACCATCT15430.1266729989483596No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG15270.12535947465595926No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT15210.12486690304630912No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCCGTGATCTTGGGAGTTACAATCTTGTCTCCTGGTACCAACAACA15200.1247848077780341No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGA15060.12363547402218379No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14330.11764251943810713No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG13740.11279889860988082No Hit
GTGTCTGGTGGCTCCACCAGTTATTACTTTTGGAGTTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATT13640.11197794592713059No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13100.10754480144027939No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13050.10713432509890428No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT12660.1039326096361784No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA12630.10368632383135333No Hit
CCTTGGTCCCTCCGCCGAACACCACATAAGTTTTACTACCTGCATATGAACAACAATAATAAGTGGCCTCGTCTT12570.10319375222170318No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA12480.10245489480722798No Hit
GCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA12430.10204441846585288No Hit
GGATGACATTAAGCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCA12350.10138765631965271No Hit
GCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCCGT12300.10097717997827758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)