FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981313
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTATGATAGTTCACCTCCAACGTGGACGTTCGGCC55190.5624097510172595No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT36180.3686897045081437No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32720.33343082176634775No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32120.32731656464349296No Hit
GTGTATTACTGTCAGCACTATGATAGTTCACCTCCAACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAA23240.2368255592252421No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT22870.23305510066614832No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22310.22734846068481718No Hit
GTCTCTGGTGGCTCCTTCACCACTACTACTTACTACTGGAACTGGATCCGGCAGTCTGCCGGGAAGGGCCTGGAG21750.22164182070348606No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21180.215833276436774No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC19720.20095525077116067No Hit
CTCCTGCAGGGCCAGTCAGAATGTTGGCAGGACCTTCTTAGCCTGGTACC19230.19596194078749593No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19140.19504480221906773No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCACTA17850.18189914940492993No Hit
CTATGATAGTTCACCTCCAACGTGGACGTTCGGCCAAGGGACCAAGGTGG17740.18077820226573987No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17700.18037058512421622No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17420.17751726513355065No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT17190.17517346656978966No Hit
GTCCTGGACCTCCTGTGCAAGAACATGGAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC16950.17272776372064774No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16740.17058777372764858No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16700.1701801565861249No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16630.16946682658845852No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16360.16671541088317388No Hit
CCTCATGTATGATCCATCTAGCAGGGCCAGTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACT16020.16325066518022283No Hit
ATCTAGCAGGGCCAGTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG15780.1608049623310809No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15150.15438499235208336No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15120.15407927949594064No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT15020.15306023664213153No Hit
TTCTTAGCCTGGTACCAGCAGACACCTGGCCAGGCCCCCAGGCTCCTCATGTATGATCCATCTAGCAGGGCCAG14990.15275452378598878No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC14760.1504107252222278No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCAGGACCTTCTTAGCC14740.15020691665146593No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14590.14867835237075225No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14040.14307361667480203No Hit
CACCTGCACTGTCTCTGGTGGCTCCTTCACCACTACTACTTACTACTGGA13920.14185076525023108No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGTTGGAGG13780.1404241052548983No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCACTGGCCCTGCTAGATGGATCATACATGAGGAGCC13720.1398126795426128No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCAGGA13720.1398126795426128No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGTTGGAGGTGAACTATCATAG13460.13716316812270907No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG13330.1358384124127572No Hit
CTATCATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC12990.13237366670980616No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT12580.1281955910091887No Hit
GTCTACTGAGATGGTGACTCGACTTTTGAGGGAGGGGTTGTAGTTGGTGCTCCCACTGAAATAGACACGCCCAA12550.12788987815304598No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12510.12748226101152232No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12070.12299847245476214No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12000.12228514245709575No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11900.12126609960328662No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11600.11820897104185923No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11500.11718992818805009No Hit
ATCATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGC11220.11433660819738453No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11160.11372518248509904No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11120.1133175653435754No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11070.11280804391667082No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11050.11260423534590899No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA10920.11127947963595712No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10820.110260436782148No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10810.11015853249676708No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCACTATGATAGTTCA10790.10995472392600525No Hit
TGTATGATCCATCTAGCAGGGCCAGTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCAC10670.10873187250143429No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG10590.107916638218387No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA10380.10577664822538782No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10310.10506331822772144No Hit
TACATGAGGAGCCTGGGGGCCTGGCCAGGTGTCTGCTGGTACCAGGCTAAGAAGGTCCTGCCAACATTCTGAC10270.10465570108619777No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10180.10373856251776957No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC10140.1033309453762459No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9950.10139476395400857No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCACTGGCCCTGCTAGATGGATCATACA9940.10129285966862765No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9880.10068143395634216No Hit
CACCTGGCCAGGCCCCCAGGCTCCTCATGTATGATCCATCTAGCAGGGCCAGTGGCATCCCAGACAGGTTCAG9880.10068143395634216No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGAAGAAATTGAGTTGACGCAGTCTC9870.10057952967096125No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9820.1000700082440567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACGA17050.058.11262570
GACGACT200.006917794252.5648856
TGTTAGA6650.051.1701870
GTATAAC200.00791374550.8068241
GTTATTA600.00839821250.04142870
TCGTATG1550.048.68840840
ACGTAGA1750.048.45414727
ATCTCGT1300.047.32811437
CGTATGC1600.047.2278641
GACGTAG1800.047.0889726
CGTAGAG1850.045.86076428
AGACGTA1850.045.79759625
ATCTCCG1850.045.74392710
ATTCAGA1352.3403281E-644.48126670
CAGTTCG14400.043.7862570
CACGAGA2000.042.33470521
ATGCCGT1800.042.12187244
GGTATCA9100.042.0598641
GTATGCC1800.042.03682342
CGAGACG2050.041.31057423
Produced by FastQC (version 0.11.5)