FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283850
Sequences flagged as poor quality0
Sequence length18-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG52930.41227557736495696No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGCTGATGTCATACGA48650.37893834949565763No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG47290.36834521166802975No Hit
CTCCTGCACTGGGAGCAGTTCCAACGTCGGGGCAGGCTTTGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC44970.3502745647856058No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACCACTCAGG41760.3252716438836312No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC39950.31117342368656775No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG39520.30782412275577364No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT35450.27612259999221095No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT34620.2696576702885851No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31130.2424738092456284No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28060.21856135841414495No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26770.20851345562176268No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26700.2079682205865171No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26420.20578728044553493No Hit
ACATCAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCC26280.2046968103750438No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26060.20298321454998636No Hit
GGCTGATGTCATACGACTGGCAGTGATAATCAGCCTCATCCTCAGCCTGG25970.2022821980760992No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25630.1996339136191923No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25600.1994002414612299No Hit
GGATGAGGCTGATTATCACTGCCAGTCGTATGACATCAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGG24890.1938700003894536No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24490.19075437161662187No Hit
GTGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGTTCCAACGTCGGGGCAGGCTTTGA24480.19067648089730108No Hit
GTTCCAACGTCGGGGCAGGCTTTGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATTT24370.18981968298477236No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24200.18849554075631889No Hit
TCATTGGCCTCCAGGCTGAGGATGAGGCTGATTATCACTGCCAGTCGTATGACATCAGCCTGAGTGGTTGGGTGT23500.18304319040386338No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG22670.17657826070023758No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC22320.1738520855240098No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC22280.17354052264672665No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22270.17346263192740585No Hit
GATTATCACTGCCAGTCGTATGACATCAGCCTGAGTGGTTGGGTGTTCGG22080.17198270826031078No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21950.17097012890914048No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT21600.1682439537329127No Hit
GTGCAGGAGATGGTGACCCTCTGCCCGGGGGCCCCAGACACTGAGGGCGG21450.16707559294310084No Hit
CCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGCTGATGTCATACGACTGGCAGTGATAATCAGCCTCATCC21160.16481676208279783No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAG21010.16364840129298594No Hit
GATCTATACCACTGGGAGCATCAACTTCAACCCCTCCCTCACGAGTCGAG20860.16248004050317405No Hit
CAGTAATAGAAGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCGTCATGGAGAACTGGTTCTTGGACTTGTCAA20790.1619348054679285No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC20790.1619348054679285No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20260.15780659734392646No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19860.15469096857109477No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT19730.15367838921992444No Hit
CCCAACCACTCAGGCTGATGTCATACGACTGGCAGTGATAATCAGCCTCATCCTCAGCCTGGAGGCCAATGA19250.14993963469252639No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18840.14674611520037387No Hit
GTAATAGAAGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCGTCATGGAGAACTGGTTCTTGGACTTGTCAACTGA18710.14573353584920357No Hit
GCCTCATCCTCAGCCTGGAGGCCAATGATGGCCAGGGAGGCTGAGGTGCC18500.1440978307434669No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAC18440.14363048642754217No Hit
GCCCCCAAACTCCTCATTTTTGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGGTTCTCTGGCTCCAAG18350.14292946995365502No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGC17900.13942438758421932No Hit
GTGTACATCAAAGCCTGCCCCGACGTTGGAACTGCTCCCAGTGCAGGAGA17630.13732133816255793No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCATTGGCCTCCAGGCTGAGGATGAGGCTGATTATCACTGCCAGTCGTA17630.13732133816255793No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17490.13623086809206683No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17380.13537407017953812No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT17270.13451727226700938No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17190.13389414651244305No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17180.13381625579312223No Hit
GTGATAATCAGCCTCATCCTCAGCCTGGAGGCCAATGATGGCCAGGGAGG17020.13257000428398957No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16950.132024769248744No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG16930.13186898781010242No Hit
CTTTAATTCAGTCCCAGGGGTGTCTCTCACACAGTAATAGAAGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCG16860.13132375277485686No Hit
TCCAGGAACAGCCCCCAAACTCCTCATTTTTGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGGTTCTC16850.1312458620555361No Hit
CTATTACTGTGTGAGAGACACCCCTGGGACTGAATTAAAGTGGGGCCAGG16320.12711765393153407No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16190.12610507458036374No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16120.12555983954511818No Hit
GTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTACATCAAAGCCTGCCCCGACGTTGGAACTGCTCCCAG16110.1254819488257974No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT15920.12400202515870234No Hit
TCCTCAGCCTGGAGGCCAATGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAACCGGTCAGGGACC15750.12267788293024887No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG15700.1222884293336449No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15460.12041905206994588No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15380.11979592631537951No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15200.11839389336760525No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15170.11816022120964287No Hit
GGCTTTGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATTTTTGGTAACACCAATCGG14830.11551193675273591No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14790.11520037387545273No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG14760.11496670171749036No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14570.11348677805039531No Hit
GTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCATTGGCCTCCAGGCTGAGGATGAGGCTGATTA14460.11262998013786658No Hit
GGCCATCATTGGCCTCCAGGCTGAGGATGAGGCTGATTATCACTGCCAGTCGTATGACATCAGCCTGAGTGGT14360.11185107294465865No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14280.1112279471900923No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14200.11060482143552595No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC14170.11037114927756357No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTC14030.10928067920707248No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13980.1088912256104685No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGTTCCAACGTCGGGGCAGGCTTTGATGTACACTGGTACCAGCAGCTTC13940.10857966273318534No Hit
GTGCCAGACTTGGAGCCAGAGAACCGGTCAGGGACCCCTGAGGGCCGATTGGTGTTACCAAAAATGAGGAGTT13820.10764497410133582No Hit
GCCCTCAGGGGTCCCTGACCGGTTCTCTGGCTCCAAGTCTGGCACCTCAG13680.10655450403084474No Hit
CTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTGATGGTGGCAGCTCCCAGATGGGTC13410.10445145460918331No Hit
GTGTCTGGTGTCTCCATCGGTAGTCATTATTGGAGCTGGATCCGGCAGCCCGCCGGGAAGGGACTGGAGTGGAT13370.10413989173190014No Hit
GCCTGGAGGCCAATGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC13310.10367254741597538No Hit
CCCTCAGTGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGTTCCAACGTCGGGGCAGG13240.10312731238072982No Hit
ATGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAACCGGTCAGGGACCCCTGAGGGCCGATTGGTG13140.1023484051875219No Hit
GGCTGAGGATGAGGCTGATTATCACTGCCAGTCGTATGACATCAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGAC13130.10227051446820112No Hit
CCCCTGAGGGCCGATTGGTGTTACCAAAAATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTA12910.10055691864314367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4400.0140.8597770
CCGGTAA200.001227470381.3164469
CGCGTAT900.057.04207239
ATCGCGT850.056.24254637
TATCGCG1050.055.23179236
GTATCGC1050.055.18198435
ACGTTTG200.006563313353.2696463
GCGTATG1000.051.3884540
TCTCGCG9650.050.7049170
TCGCGTA950.050.37769338
AGTATCG1300.049.77066434
CGTATGC4550.049.0147941
AAAGGGG4950.047.84267468
TTATACA7050.045.6162072
TGGTATC2550.045.1360362
CGATCAG7000.045.08545729
TATACAC7400.044.8310663
ATGCCGG851.8189894E-1244.5793644
ACCGATC6850.044.5569427
ACGAGAC6800.044.31841322
Produced by FastQC (version 0.11.5)