FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283850
Sequences flagged as poor quality0
Sequence length18-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75700.5896327452584025No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT50950.39685321493944No Hit
CTCCTGCACTGGGAGCAGTTCCAACGTCGGGGCAGGCTTTGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC41930.32659578611208473No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41080.31997507496981736No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGCTGATGTCATAC39530.30790201347509444No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38760.3019044280873934No Hit
GTCCTGGGCCCAGTCTGTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCCT37920.29536160766444675No Hit
GTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCCGGGCAGAGGGTCAC34200.2663862600771118No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATTTTTGGTAACACCAATCGGCCCTCA33920.2642053199361296No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC30390.2367098960158897No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30210.2353078630681154No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT30110.23452895587490752No Hit
GTTCCAACGTCGGGGCAGGCTTTGATGTACACTGGTACCAGCAGCTTCCA29220.22759668185535695No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29100.22666199322350744No Hit
AGTCTGTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCA29050.22627253962690347No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACCACTCAGG28300.22043073567784396No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA27490.21412158741285978No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA25700.20017914865443784No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG25660.19986758577715466No Hit
ACATCAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT24690.19231218600303773No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24330.1895081201074892No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23790.18530202126416637No Hit
GGATGAGGCTGATTATCACTGCCAGTCGTATGACATCAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGGT23070.17969388947306927No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22890.178291856525295No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA22780.1774350586127663No Hit
GATCTATACCACTGGGAGCATCAACTTCAACCCCTCCCTCACGAGTCGAGTCACCATGTCAGTTGACAAGTCCAA22730.17704560501616232No Hit
GCACAGGGTCCTGGGCCCAGTCTGTACTGACGCAGCCGCCCTCAGTGTCT22570.17579935350702963No Hit
TCATTGGCCTCCAGGCTGAGGATGAGGCTGATTATCACTGCCAGTCGTAT22370.17424153912061377No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG22020.17151536394438605No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCCGGGCAGAGGGTCAC21130.16458308992483547No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20730.16146746115200375No Hit
GATTATCACTGCCAGTCGTATGACATCAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTA20350.1585076138178136No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG20160.15702769015071852No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT20130.15679401799275616No Hit
GGCTGATGTCATACGACTGGCAGTGATAATCAGCCTCATCCTCAGCCTGGAGGCCAATGATGGCCAGGGAGGCT20100.15656034583479378No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG20070.15632667367683142No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG19820.1543794056938116No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT19790.1541457335358492No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG19540.1521984655528294No Hit
GTGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGTTCCAACGTCGGGGCAGGCTTTGAT19360.1507964326050551No Hit
CAGTAATAGAAGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCGTCATGGAGAACTGGTTCTTGGACTTGTCAA19320.15048486972777192No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG19280.15017330685048877No Hit
GTAATAGAAGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCGTCATGGAGAACTGGTTCTTGGACTTGTCAACT19070.1485376017447521No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT18290.14246212563773028No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG17830.1388791525489738No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17080.1330373485999143No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC17030.13264789500331034No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT17020.13257000428398957No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCATTGGCCTCCAGGCTGAGGATG16980.1322584414067064No Hit
GGGTCCTGGGCCCAGTCTGTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTC16690.12999961054640338No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT16480.12836390544066673No Hit
GGCTTTGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATTTTTGGTAACACCAATCG16370.12750710752813804No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC16240.1264945281769677No Hit
CCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGCTGATGTCATACGACTGGCAGTGATAATCAGCCTCATCCT15970.12439147875530629No Hit
GCCCCCAAACTCCTCATTTTTGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGGTTCTCTGGCTCCAAGT15890.12376835300073996No Hit
GTCTGTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCCGGGCAGAGGG15880.12369046228141918No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACCACTCA15820.12322311796549443No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC15820.12322311796549443No Hit
GAATTAAAGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCT15790.12298944580753203No Hit
TCCAGGAACAGCCCCCAAACTCCTCATTTTTGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGGTTCTCTG15740.12259999221092806No Hit
CTATTACTGTGTGAGAGACACCCCTGGGACTGAATTAAAGTGGGGCCAGG15580.12135374070179539No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15330.11940647271877557No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15170.11816022120964287No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAC15150.11800443977100128No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC15090.11753709545507653No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG15050.11722553257779336No Hit
GCCCTCAGGGGTCCCTGACCGGTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCATTGGCCTCCA15020.11699186041983096No Hit
GTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCATTGGCC14900.11605717178798146No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT14660.11418779452428242No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG14530.11317521517311212No Hit
CTTTAATTCAGTCCCAGGGGTGTCTCTCACACAGTAATAGAAGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCGT14530.11317521517311212No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA14470.11270787085718736No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14230.11083849359348834No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGG14210.11068271215484676No Hit
GTGCAGGAGATGGTGACCCTCTGCCCGGGGGCCCCAGACACTGAGGGCGGCTGCGTCAGTACAGACTGGGCCCAG14070.10959224208435564No Hit
GGCCATCATTGGCCTCCAGGCTGAGGATGAGGCTGATTATCACTGCCAGTCGTATGACATCAGCCTGAGTGGTTG13850.10787864625929822No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGTTCCAACGTCGGGGCAGGCTTTGATGTACACTGGTACCAGCAGCTTC13790.10741130194337344No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG13590.10585348755695759No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC13580.10577559683763679No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13550.10554192467967442No Hit
GGCTGAGGATGAGGCTGATTATCACTGCCAGTCGTATGACATCAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGAC13530.10538614324103283No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT13460.10484090820578729No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13260.10328309381937141No Hit
GTGTCTGGTGTCTCCATCGGTAGTCATTATTGGAGCTGGATCCGGCAGCCCGCCGGGAAGGGACTGGAGTGGATT13230.10304942166140904No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13080.10188106087159715No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13070.10180317015227634No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGA12900.10047902792382286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA3450.054.16794670
TCTAAAA900.0072634851.91095470
AGTGATA22200.050.5079570
CGGCAAA1254.247293E-449.83451570
CTCAGCA15800.046.32559670
AGGTACG306.997689E-445.0467762
GGTATCA10000.043.9531361
CGGGTAA2858.763891E-943.71448570
ATGGCAA2203.2142925E-642.472670
GGTAGAA30100.042.4255670
GTATCAA26900.040.2201961
GAGGGCA2009.1225345E-538.93321670
TCATTTT32500.038.3342470
TCTTGTT4601.0913936E-1137.24046770
TGGTATC2650.036.9723552
TACGTAC558.512279E-636.87225312
ATACGTA558.521383E-636.8665111
GACAGGT3050.036.581421
CTATAGG600.002056642436.17691869
TGACAGT34550.035.6089670
Produced by FastQC (version 0.11.5)