FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141673
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAATTACCCTCGGACGTTTGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCA47670.4175451289467299No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT46140.40414374343616777No Hit
TTATTATACTGCTGACAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC45050.39459635114432945No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44720.3917058562302866No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT29750.2605824960387081No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28660.2510351037468697No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28010.24534170467375507No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27180.23807167201116256No Hit
GCATAAACCAGGGAAAGCCCCTAAACTCCTCATGGATAAGACGTCGATGTTAGCAGTCGGGGTCCCATCAAGAT26810.23483081407723577No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25860.2265096923549913No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGGCAATTGACAAGTGG25240.2210790655467897No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25170.22046593026199274No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24830.2174878445929789No Hit
GTTTAGGGGCTTTCCCTGGTTTATGCTGATACCAGGCCAACCACTTGTCAATTGCCTGACTGGCCCGGCAAGTGA24210.21205721778477724No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC24040.21056817495027036No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23810.2085535875859375No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23200.2032105515327068No Hit
GTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGGCAATTGACAAGTGGTTGGCCTGGTA22930.20084560114848998No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22600.19795510623444715No Hit
GGTTTATGCTGATACCAGGCCAACCACTTGTCAATTGCCTGACTGGCCCGGCAAGTGATAGTGAC22490.19699160792976622No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22440.19655365415491127No Hit
GTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGGCAATTGACAAGTGGTTGGCCTGGTATCAGCATAAA22190.19436388528063642No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT21440.18779457865781182No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT21260.18621794506833392No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21100.18481649298879801No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC20080.1758822359817566No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19960.17483114692210466No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT19110.16738593274957014No Hit
CCCCTAAACTCCTCATGGATAAGACGTCGATGTTAGCAGTCGGGGTCCCATCAAGATTCAGCGGCAGTGGATC19070.16703556972968617No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18150.1589772202723547No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17970.1574005866828768No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAGACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC17540.15363418421912406No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCAAACGTC17210.15074368930508122No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16880.14785319439103842No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16810.14724005910624144No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATG16600.1454006532518506No Hit
GAAATAGACGGCCGTGTCCGCAGAGGTCACAGATCTCAAATTCAGGGAGAACTGATTCTGGGACGTGTCCACTGA16520.1446999272120826No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16290.14268533984774975No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTATTACTGTCAGCAGTATAATAATTAC16160.14154666003312683No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATTCAGATGACCCAGTCTCCTTCCACC15980.13997002644364892No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15980.13997002644364892No Hit
GTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAATCTTGATGGGAC15830.13865616511908402No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15220.13331312906585335No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15160.13278758453602738No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15130.1325248122711144No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15030.13164890472140447No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG15010.13147372321146247No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14750.12919636358221664No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14680.12858322829741967No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA14490.1269190039529708No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14350.12569273338337686No Hit
GTCTATTTCTGTGCGGGCGCTCCTTATGATTTTTGGAGTGGAAATTCCGCGGTGGACCAGGGGTATTTCGACCCT14260.1249044165886379No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14250.12481682583366692No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG14150.12394091828395695No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14090.12341537375413099No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14080.12332778299916No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTT14010.12271464771436305No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13890.12166355865471112No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCGACTGCTAACATCGACGTCTTATCCA13670.11973656204534923No Hit
CTACAACCCCTCCCTCAAGAGTCGAGTCACTATGTCAGTGGACACGTCCC13620.11929860827049428No Hit
ACTTATTACTGTCAGCAGTATAATAATTACCCTCGGACGTTTGGCCAAGGGACCAAGGTGGAAGTCAAACGAA13560.11877306374066829No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13500.11824751921084233No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG13030.1141307537272056No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG13000.11386798146229263No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC12900.11299207391258267No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA12880.1128168924026407No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12810.11220375711784372No Hit
CAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG12690.1111526680581918No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12420.10878771767397495No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12300.10773662861432301No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG12190.10677313030964207No Hit
GTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG12100.10598481351490313No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG12020.10528408747513518No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11910.10432058917045424No Hit
CACTATCACTTGCCGGGCCAGTCAGGCAATTGACAAGTGGTTGGCCTGGTATCAGCATAAACCAGGGAAAGCCC11800.1033570908657733No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11720.10265636482600533No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG11720.10265636482600533No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT11680.10230600180612137No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11640.1019556387862374No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11520.10090454972658547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)