FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318826
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT56570.42894210456876036No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54520.4133979766853247No Hit
ATCCAGGGAAAGCCCCGAAGCTCCTGATCTATGCTGCATCCAATTTGGAGAGTGGTGTCCCATCAAGGTTCAG46050.3491741897718122No Hit
GTGTTGATGGTGAGAGTGAACTCTGTCGTAGATCCACTGCCACTGAACCTTGATGGGACACCACTCTCCAAAT42210.3200573843706448No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT37680.28570865299895515No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37620.2852537029145619No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG36780.2788844017330565No Hit
CCATCAACACTCTGCAACCTGAAGATTTTGGAACTTACTACTGTCAACAG34410.26091387339952354No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33720.25568194742900124No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32430.2459005206145466No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32420.24582469560048104No Hit
GAGTTACAATACCCCGAGGACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCA32000.24264004500972836No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31070.23558831870163313No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30490.2311904678858318No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30420.23065969278737303No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC29460.22338049143708116No Hit
GTAATAGATGGCCGTGTCCGCGGCAGTCACAGACGTCAGGTTCAGGGAGAACTGGTTGTTAGAAGTGTCAATTGA29190.22133321605731157No Hit
GATCTATGCTGCATCCAATTTGGAGAGTGGTGTCCCATCAAGGTTCAGTGGCAGTGGATCTACGACAGAGTTCA29120.2208024409588528No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28640.2171628402837069No Hit
GTCTCCAATTGTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCGTTTCCAC28050.21268916445384No Hit
GTTACAATACCCCGAGGACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCT27880.2114001392147258No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCGTTTCCACCTATTTAAATTGGTATCAGCACAA27230.2064715133004657No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC25570.19388456096558607No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG25360.19229223567020973No Hit
TCCCTGGATTGTGCTGATACCAATTTAAATAGGTGGAAACGCTCTGATTTGCCCGGCAAGTGATGGTGAC25140.19062408536076783No Hit
ACAATACCCCGAGGACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCA24590.18645370958716312No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24430.18524050936211447No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT24060.1824349838416895No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACGTCCAGCTGACGCAGTC22720.17227443195690714No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTC22610.1714403568021862No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTCCTCGGGGTAT22580.17121288175998955No Hit
ATCTACGACAGAGTTCACTCTCACCATCAACACTCTGCAACCTGAAGATTTTGGAACTTACTACTGTCAACAGAG21900.16605678080353284No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACG21370.1620380550580592No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG20950.15885340446730653No Hit
GTATTGTAACTCTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGAGTGTTGATGGTGAGAGTGAAC20830.15794350429852003No Hit
GCATAGATCAGGAGCTTCGGGGCTTTCCCTGGATTGTGCTGATACCAATTTAAATAGGTGGAAACGCTCTGATT20760.15741272920006127No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20630.15642700401720924No Hit
GTGGTGTCCCATCAAGGTTCAGTGGCAGTGGATCTACGACAGAGTTCACT20260.15362147849678426No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19730.14960275275131063No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19290.14626645213242687No Hit
GGGTATTGTAACTCTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGAGTGTTGATGGTGAGAGTGAAC19140.14512907692144375No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19040.14437082678078836No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18650.14141365123223232No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18400.1395180258805938No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17920.13587842520544788No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCGTTTCCACCTATTTAAATTGGTA17660.13390697483974381No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA17590.13337619974128506No Hit
GTCTCTGGTCACTCCGTGAGCACTCCAAATTACTACTGGACCTGGATCCGCCAACTCCCAGGACAGGGCCTGGAG17090.12958494903800805No Hit
GTCGTAGATCCACTGCCACTGAACCTTGATGGGACACCACTCTCCAAATT17070.12943329900987696No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16920.12829592379889387No Hit
GAGTTCACTCTCACCATCAACACTCTGCAACCTGAAGATTTTGGAACTTACTACTGTCAACAGAGTTACAATACC16360.12404972301122362No Hit
GCACAATCCAGGGAAAGCCCCGAAGCTCCTGATCTATGCTGCATCCAATTTGGAGAGTGGTGTCCCATCAAGGT16080.1219266226173885No Hit
ATCTATTACTGTGCGAGGGTCTCACCTGCTATCATCGCCTACGACCACTGGGGCCAGGGAATCCTGGTCACCG16050.12169914757519187No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGAGT16020.12147167253299526No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15990.12124419749079865No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15930.1207892474064054No Hit
GATCAGGAGCTTCGGGGCTTTCCCTGGATTGTGCTGATACCAATTTAAAT15890.12048594735014323No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACGTCCAGCTGACGCAGTCTCCAATTGTC15770.11957604718135675No Hit
CTCCTGATCTATGCTGCATCCAATTTGGAGAGTGGTGTCCCATCAAGGTTCAGTGGCAGTGGATCTACGACAGAG15470.11730129675939056No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT15460.117225471745325No Hit
GGATTGTGCTGATACCAATTTAAATAGGTGGAAACGCTCTGATTTGCCCGGCAAGTGATGGTGAC15260.1157089714640142No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15050.11411664616863787No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15000.11373752109831016No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG14900.11297927095765475No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14790.11214519580293382No Hit
GAGTGAACTCTGTCGTAGATCCACTGCCACTGAACCTTGATGGGACACCACTCTCCAAATTGGATGCAGCATAGA14700.11146277067634397No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14690.11138694566227843No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14510.1100220954090987No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14460.10964297033877099No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14360.1088847201981156No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14320.10858142014185344No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG14250.10805064504339466No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG14230.10789899501526358No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14110.10698909484647709No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13990.10607919467769061No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT13780.10448686938231426No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT13770.10441104436824872No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13750.10425939434011765No Hit
CTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCA13700.10388026926978997No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13610.10319784414320007No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13400.10160551884782375No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13340.1011505687634305No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13230.10031649360870956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAAG550.00564244255.33886370
AATGGTA6250.055.1912970
GGCGTAA300.005926446554.66045869
AGTATCG900.045.38500234
TCTCGCG10900.044.6772570
GTATCGC705.020411E-1043.80420335
CGCGTAT504.075184E-641.04845439
TATCGCG759.786163E-1040.91515736
GTATCAA31700.038.4433981
ACCGATC5150.038.18490227
GACCGAT5150.038.1689526
ACGAGAC5600.038.09599322
ATACGGA450.001623329137.96381468
CTTATAC5500.036.9289631
TGAGCGG3850.036.8925870
TCAGTAT5550.036.75099632
TATACAC5850.036.443073
TCGCGTA754.3819455E-836.44281838
AGACCGA5400.036.3963425
CACGAGA5800.036.19574421
Produced by FastQC (version 0.11.5)