FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318826
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55070.4175683524589294No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52190.3957307484080538No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA50510.38299214604504306No Hit
ATCCAGGGAAAGCCCCGAAGCTCCTGATCTATGCTGCATCCAATTTGGAGAGTGGTGTCCCATCAAGGTTCAGTG42560.32271125986293875No Hit
GTGTTGATGGTGAGAGTGAACTCTGTCGTAGATCCACTGCCACTGAACCTTGATGGGACACCACTCTCCAAATTG41930.3179342839768097No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37480.28419215271764436No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT35830.2716810253968302No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34940.2649325991449971No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC33680.2553786473727391No Hit
CCATCAACACTCTGCAACCTGAAGATTTTGGAACTTACTACTGTCAACAGAGTTACAATACCCCGAGGACTTTCG30640.2323278430968149No Hit
GAGTTACAATACCCCGAGGACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT30140.22853659239353788No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC29650.22482116670432645No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29640.22474534169026086No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA28800.2183760405087555No Hit
GTTACAATACCCCGAGGACTTTCGGCCCTGGGACCAAAGTGGAAATCAAA28740.21792109042436228No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28520.2162529401149204No Hit
GATCTATGCTGCATCCAATTTGGAGAGTGGTGTCCCATCAAGGTTCAGTGGCAGTGGATCTACGACAGAGTTCA28230.21405401470701974No Hit
GTAATAGATGGCCGTGTCCGCGGCAGTCACAGACGTCAGGTTCAGGGAGAACTGGTTGTTAGAAGTGTCAATT27140.20578908817387587No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA25970.19691756152820766No Hit
GTCTCCAATTGTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCGTTTCCAC25920.19653843645787997No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC25650.19449116107811038No Hit
TCCCTGGATTGTGCTGATACCAATTTAAATAGGTGGAAACGCTCTGATTTGCCCGGCAAGTGATGGTGACT23970.18175255871509963No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCGTTTCCACCTATTTAAATTGGTATCAGCACAAT23950.18160090868696857No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23820.18061518350411654No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23820.18061518350411654No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23550.17856790812434695No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT23190.17583820761798752No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA23050.17477665742106996No Hit
ACAATACCCCGAGGACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGA21940.166360080859795No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACGTCCAGCTGACGCAGTCTCCAATTGTCCTGTCTGCATCTGTA21740.1648435805784842No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTC21670.16431280548002541No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21460.16272048018464907No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGT20550.1558204039046849No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACGT20310.15400060356711198No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTCCTCGGGGTATT20130.15263575331393223No Hit
ATCTACGACAGAGTTCACTCTCACCATCAACACTCTGCAACCTGAAGATT20120.1525599282998667No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20050.15202915320140792No Hit
GTGGTGTCCCATCAAGGTTCAGTGGCAGTGGATCTACGACAGAGTTCACTCTCACCATCAACACTCTGCAACCT19910.15096760300449036No Hit
GCATAGATCAGGAGCTTCGGGGCTTTCCCTGGATTGTGCTGATACCAATTTAAATAGGTGGAAACGCTCTGATTT19840.15043682790603158No Hit
GTATTGTAACTCTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGAGTGTTGATGGTGAGAGTGAACT18860.14300597652760866No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18650.14141365123223232No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA18590.14095870114783907No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18580.14088287613377354No Hit
GGGTATTGTAACTCTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGAGTGTTGATGGTGAGAGTGAACT18310.13883560075400395No Hit
GTCGTAGATCCACTGCCACTGAACCTTGATGGGACACCACTCTCCAAATTGGATGCAGCATAGATCAGGAGCTTC17490.13261794960062967No Hit
GAGTTCACTCTCACCATCAACACTCTGCAACCTGAAGATTTTGGAACTTACTACTGTCAACAGAGTTACAATACC16980.1287508738832871No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGA16890.12806844875669726No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16830.127613498672304No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT16270.12336729788463377No Hit
CTCCTGATCTATGCTGCATCCAATTTGGAGAGTGGTGTCCCATCAAGGTT15850.12018264729388108No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACGTCCAGCTGACGCAGTCTCCAATTGTC15550.11790789687191489No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15500.11752877180158718No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15490.11745294678752163No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15490.11745294678752163No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15280.11586062149214528No Hit
ATCTATTACTGTGCGAGGGTCTCACCTGCTATCATCGCCTACGACCACTGGGGCCAGGGAATCCTGGTCACCGT15150.11487489630929326No Hit
GTCTCTGGTCACTCCGTGAGCACTCCAAATTACTACTGGACCTGGATCCG15000.11373752109831016No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGAGTGTTGATGGTGAGAGTGAACTCTGTC14700.11146277067634397No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14670.11123529563414736No Hit
GCACAATCCAGGGAAAGCCCCGAAGCTCCTGATCTATGCTGCATCCAATTTGGAGAGTGGTGTCCCATCAAGGTT14660.11115947062008179No Hit
GAGTGAACTCTGTCGTAGATCCACTGCCACTGAACCTTGATGGGACACCACTCTCCAAATTGGATGCAGCATA14600.11070452053568856No Hit
GGATTGTGCTGATACCAATTTAAATAGGTGGAAACGCTCTGATTTGCCCGGCAAGTGATGGTGACT14580.11055287050755747No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14550.11032539546536085No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG14430.10941549529657438No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14410.10926384526844331No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC14410.10926384526844331No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCA14190.10759569495900141No Hit
GATCAGGAGCTTCGGGGCTTTCCCTGGATTGTGCTGATACCAATTTAAATAGGTGGAAACGCTCTGATTTGCCCG14000.10615501969175616No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA13960.10585171963549399No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13860.10509346949483858No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA13820.10479016943857643No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA13670.10365279422759333No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13510.10243959400254468No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT13460.10206046893221699No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13370.10137804380562714No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAG13270.10061979366497173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA4750.053.76335570
TCGGTTA200.0080187250.638714
CCGCTAT850.00837640750.07370870
CTGGAGT13550.042.92912370
CTGTCTT32350.041.663670
ATGGCAA2801.7078492E-840.53586270
TAGGCAA3609.094947E-1139.40986370
GTATCAA29300.039.2026671
GTGGTAT3150.038.6096841
GAGCAAA29500.037.99378270
AACTGTA2257.117518E-637.83347370
AAACTCA3654.5092747E-934.98300670
TATCAAC34450.033.1221662
AAGCGGT9500.032.85538570
ATCAACG35100.032.306633
TCAACGC35400.032.031634
CAACGCA35900.031.5855085
AACGCAG36200.031.2305266
CCGAGGT44400.030.99532570
CCAGAGT9700.030.71531970
Produced by FastQC (version 0.11.5)