FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806659
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG29550.3663257956583885No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACATAAGTACCACTGTCTGCTGATTGA29320.3634745288901506No Hit
ATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGT26850.33285440316168297No Hit
GTCTTTATATATCACCAACACAGGGGCCTGGCCTGGCTTTTGCTGGTACC26790.33211059443953395No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25810.3199617186444334No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACATAAGTACCACTGTCTGCTGATTGACAGTAATAG24870.30830871533076554No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23810.2951680945727997No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG22010.2728538329083293No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGCCAAAG20450.253514806132455No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT19390.24037418537448907No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC19070.23640720552302769No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGA18030.22351452100577815No Hit
GACTATTACTGTCAATCAGCAGACAGTGGTACTTATGTGGTATTTGGCGGAGGGACCAAGCTGACCGTCCTAGG17590.21805992371001873No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17510.2170681787471534No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA17100.21198548581246845No Hit
GTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCG16460.20405152610954566No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG16430.20367962174847118No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16320.2023159724245313No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15940.1976051838509209No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG15770.19549772580483202No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15300.1896712241479981No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15150.18781170234262556No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14990.18582821241689487No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14790.1833488500097315No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14700.18223313692650797No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14560.18049758324149362No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14470.1793818701582701No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC14160.17553885842716688No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13540.1678528349649604No Hit
AAGTACCACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA13470.16698505812245323No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC13390.16599331315958787No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGCCAAAGCAATATGCTTATTGG13280.16462966383564803No Hit
CTATTACTGTCAATCAGCAGACAGTGGTACTTATGTGGTATTTGGCGGAG13260.1643817275949317No Hit
GAGTAGTATTGCTTCGCCAATCCAAAGTCGGGACCCCTCGCACAGTAATACACGGCCGTGTCCTCAGATCTCAGG13190.1635139507524245No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG13170.16326601451170816No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13000.1611585564656193No Hit
CAGTGGTACTTATGTGGTATTTGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGG12490.1548361823273527No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12290.15235681992018932No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT12060.14950555315195146No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTG11880.1472741269855044No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA11760.1457865095412064No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT11590.1436790514951175No Hit
GCAATATGCTTATTGGTACCAGCAAAAGCCAGGCCAGGCCCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCC11440.14181952968974498No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACATAAGTACCAC11320.14033191224544697No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11300.14008397600473063No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACATAAGTAC11100.13760461359756726No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10950.13574509179219474No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT10950.13574509179219474No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10840.13438144246825487No Hit
GTGGTACTTATGTGGTATTTGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA10830.1342574743478967No Hit
CATAAGTACCACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACG10660.1321500163018078No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG10600.13140620757965882No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10410.12905081329285362No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10210.12657145088569025No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC10160.1259516102838994No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG10090.1250838334413922No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10080.12495986532103404No Hit
GGCCAGGCCCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGG10030.12434002471924321No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9950.12334827975637784No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9860.12223256667315432No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT9850.12210859855279615No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9850.12210859855279615No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT9810.12161272607136349No Hit
ATACTACTCCATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGG9740.1207449492288563No Hit
GTACCACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA9720.12049701298813997No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9380.11628209689596224No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT9320.11553828817381323No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTA9000.11157130832235183No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8970.11119940396127732No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8910.11045559523912832No Hit
GTGTATTACTGTGCGAGGGGTCCCGACTTTGGATTGGCGAAGCAATACTA8850.10971178651697928No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8830.10946385027626294No Hit
GCTTGGTCCCTCCGCCAAATACCACATAAGTACCACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTC8820.10933988215590477No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8740.10834813719303943No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG8730.10822416907268126No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8710.10797623283196495No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8660.10735639223017408No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8510.10549687042480158No Hit
GGATCACCTGCTCTGCAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAAAAGCCAGGCCAGGCCC8480.10512496606372705No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG8460.10487702982301071No Hit
GTATTACTGTGCGAGGGGTCCCGACTTTGGATTGGCGAAGCAATACTACTCCATGGACGTCTGGGGCAAAGGGAC8440.10462909358229437No Hit
CAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGACAGTGGTA8370.1037613167397872No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8340.10338941237871269No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGACAGTGGTACTTATGTGGTATT8260.10239766741584734No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG8210.10177782681405649No Hit
ACACAGGGGCCTGGCCTGGCTTTTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTGCAGAGCAGGTGA8190.10152989057334016No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGC8140.10091004997154931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)