FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004685641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806659
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT29550.3663257956583885No Hit
ATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGTT28980.35925961279797286No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACATAAGTACCACTGTCTGCTGATT27770.34425947023463443No Hit
GTCTTTATATATCACCAACACAGGGGCCTGGCCTGGCTTTTGCTGGTACC26680.33074694511559405No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25090.3110360139786452No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23130.2867382623884442No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22130.2743414503526273No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACATAAGTACCACTGTCTGCTGATTGACAGTAATAGT21950.2721100241861803No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19710.2443411652259505No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGCCAAAG18950.2349195880787297No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18480.22909308642189574No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC18360.22760546897759773No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17620.21843182807109326No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA17590.21805992371001873No Hit
GACTATTACTGTCAATCAGCAGACAGTGGTACTTATGTGGTATTTGGCGGAGGGACCAAGCTGACCGTCCTAGGT17500.2169442106267952No Hit
GTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCG17200.21322516701605015No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGAT16960.2102499321274541No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACG16340.20256390866524765No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG16160.20033248249880062No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG15980.1981010563323536No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA15420.19115884159229612No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14870.18434059497259683No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC14630.18136536008400078No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14450.17913393391755375No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGC14170.17566282654752505No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGA13440.16661315376137872No Hit
GAGTAGTATTGCTTCGCCAATCCAAAGTCGGGACCCCTCGCACAGTAATACACGGCCGTGTCCTCAGATCTCAG13220.163885855113499No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA12950.16053871586382845No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA12730.15781141721594874No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12660.15694364037344158No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG12600.15619983165129253No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT12580.15595189541057622No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG12570.15582792729021805No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT12430.1540923736052037No Hit
CTATTACTGTCAATCAGCAGACAGTGGTACTTATGTGGTATTTGGCGGAGGGACCAAGCTGACCGTCCTAGGTCA12420.15396840548484553No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG12380.15347253300341285No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG12350.15310062864233834No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT12230.1516130111980403No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG12220.15148904307768213No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11920.14776999946693709No Hit
AAGTACCACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT11860.14702619074478807No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG11450.14194349781010315No Hit
GTGTATTACTGTGCGAGGGGTCCCGACTTTGGATTGGCGAAGCAATACTACTCCATGGACGTCTGGGGCAAAGG11430.1416955615693868No Hit
GTGGTACTTATGTGGTATTTGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA11140.13810048607899994No Hit
CAGTGGTACTTATGTGGTATTTGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT10570.1310343032185843No Hit
GCAATATGCTTATTGGTACCAGCAAAAGCCAGGCCAGGCCCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCC10300.12768716396891375No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT10270.12731525960783924No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT10190.1263235146449739No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCA10100.12520780156175038No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACATAAGTAC10030.12434002471924321No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9910.12285240727494517No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9870.1223565347935125No Hit
GGCCAGGCCCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGG9830.12186066231207983No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAG9810.12161272607136349No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCA9790.12136478983064715No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9630.11938129990491646No Hit
CATAAGTACCACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCT9570.11863749118276744No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9440.11702590561811124No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC9420.11677796937739492No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG9100.11281098952593352No Hit
GTACCACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT9050.11219114892414267No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACATAAGTACCAC8940.1108274996002028No Hit
ATACTACTCCATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT8940.1108274996002028No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC8870.10995972275769564No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8820.10933988215590477No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA8720.10810020095232309No Hit
GTATTACTGTGCGAGGGGTCCCGACTTTGGATTGGCGAAGCAATACTACTCCATGGACGTCTGGGGCAAAGGGAC8660.10735639223017408No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8620.1068605197487414No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGACAGTGGTACTTATGTGGTATTTG8490.10524893418408522No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG8460.10487702982301071No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8420.10438115734157803No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGTGCTCGTGGATTCGTCCGCG8380.10388528486014537No Hit
GTATTTGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC8330.10326544425835452No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG8320.10314147613799635No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8220.10190179493441466No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG8160.10115798621226565No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8160.10115798621226565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)