FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004685644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801212
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTAACTCACCTTTCACTTTCGGCCCTGGGACCAAGGTGGATATCAAACGAACTG40030.49961807861090446No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT27280.340484166487771No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26250.32762864260645125No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24530.30616116583376185No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18590.23202348442110202No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT18370.22927764436878129No Hit
GTGTATTACTGTCAGCAATATGGTAACTCACCTTTCACTTTCGGCCCTGGGACCAAGGTGGATATCAAACGAA17100.2134266586122025No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGG16650.20781016759609194No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16540.20643724756993156No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16370.20431546207495643No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15650.1953290764491795No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15460.19295766913126614No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14850.18534420353164954No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA14730.18384647259402007No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC14490.18085101071876108No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14460.1804765779843537No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCCGTGGCAGTGGGTCTGGGACAGACTTCA14180.17698187246321823No Hit
CTCCTGCAGGGCCAGTCAGACTGTTAGCAGCAGCTACTTAGCCTGGTACC14070.17560895243705785No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13800.1722390578273915No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAATA13780.17198943600445327No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13750.17161500327004586No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13130.16387672675896017No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG12700.15850985756578784No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12690.1583850466543187No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT12560.15676250480522008No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCAGCAGCTACTTAGCC12540.15651288298228183No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11790.1471520646220975No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC11530.14390698092390028No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11400.14228443907480168No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCAG11350.14166038451745605No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT11260.14053708631423392No Hit
GTGTATTACTGTGCGAGAGGCTGGGCGGCCTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTC11210.13991303175688832No Hit
GTCCCAGACCCACTGCCACGGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAGCC11150.13916416628807357No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACCTTGGTCC11100.13854011173072794No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC11000.1372920026160367No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT10740.13404691891783946No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA10560.13180032251139523No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACAGTCTGA10380.129553726104951No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGACACCTTCAGCAG10130.1264334533182229No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10010.1249357223805934No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAATATGGTAACTCA9870.12318836962002565No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9840.12281393688561829No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9820.12256431506268003No Hit
GTGCAGCCACAGTTCGTTTGATATCCACCTTGGTCCCAGGGCCGAAAGTG9730.12144101685945792No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA9650.12044252956770493No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9610.11994328592182843No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTA9500.11857036589566806No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9450.11794631133832244No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACGGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGA9370.11694782404656945No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9300.11607414766628557No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9030.11270425305661923No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8870.11070727847311322No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8730.1089599257125455No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8660.10808624933226162No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8610.107462194774916No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8540.10658851839463214No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8540.10658851839463214No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG8480.10583965292581739No Hit
GTATTACTGTGCGAGAGGCTGGGCGGCCTACTACATGGACGTCTGGGGCA8420.10509078745700265No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG8360.10434192198818791No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8350.10421711107671877No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG8280.1033434346964349No Hit
GTTCCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCCTGGAACTTCTGTGCGTAGTTTG8250.10296900196202752No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8140.10159608193586717No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8140.10159608193586717No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC8120.10134646011292892No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA8060.10059759464411416No Hit
ACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGGCTGGGCGGCCTACTACA8040.10034797282117591No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8020.10009835099823766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)