FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640475
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAGTCTGTTGACAGAAGTAAAATCCACAATCCTCCGGTTGCAGACCGGTGATGGTGAGAGTGAAATCTGTC34070.5319489441430189No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT28320.44217182559818885No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27860.43498965611460244No Hit
GACTTACACTATCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCT25890.40423123463054766No Hit
ATCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG20390.31835746906592766No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG18770.29306374175416683No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT18710.29212693703891646No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCA18030.28150981693274524No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17720.2766696592372848No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17410.27182950154182445No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16690.26058784495881965No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16390.2559038213825676No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15990.2496584566142316No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15240.23794839767360162No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15000.23420117881260005No Hit
CACCATCACCGGTCTGCAACCGGAGGATTGTGGATTTTACTTCTGTCAACAGACTTACACTATCTGGACGTTCGG14960.23357664233576644No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14630.22842421640188923No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC14210.22186658339513643No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT13740.21452827979234163No Hit
GTCTCCATCCTCCCTGTCTGCTTCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAA13460.2101565244545064No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC13380.2089074515008392No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAATTGATTGCTAATGGTCTGACT13250.20687770795113003No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13200.20609703735508805No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13180.2057847691166712No Hit
CGTCCAGATAGTGTAAGTCTGTTGACAGAAGTAAAATCCACAATCCTCCGGTTGCAGACCGGTGATGGTGAGAG11960.18673640657324642No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11900.18579960185799602No Hit
GATTTCACTCTCACCATCACCGGTCTGCAACCGGAGGATTGTGGATTTTACTTCTGTCAACAGACTTACACTA11790.1840821265467036No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGCGTCATACAGATGACCCAGTC11590.18095944416253562No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGGA11450.17877356649361803No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT11040.17237206760607363No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC10970.17127912877161483No Hit
GAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGGATGCAGCATAGA10840.1692493852219056No Hit
GCGTCATACAGATGACCCAGTCTCCATCCTCCCTGTCTGCTTCTATAGGAGACAGAGTCACCATCACTTGCCGGG10340.16144267926148562No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAATT10310.16097427690386043No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10140.15831999687731763No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9920.15488504625473282No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9740.15207463210898162No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9640.1505132909168976No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9490.14817127912877162No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA9480.1480151450095632No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAATTGATTGCTAATGGTCTGACTTGCCCGGCAAGTGA9460.1477028767711464No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9440.14739060853272962No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9170.14317498731410283No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT9130.14255045083726922No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAATCAATTAAATTGGTATCAGCAGAAA9090.1419259143604356No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8570.13380694016159883No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8560.1336508060423904No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGCGTCATACAGATGACCCAGTCTCCATCCTCC8380.1308403918966392No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8100.12646863655880403No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7900.12334595417463601No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACT7770.12131621062492681No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT7680.11991100355205121No Hit
ACCTTGATGGGACCCCACTTTGCAAATTGGATGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTC7600.11866193059838401No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7600.11866193059838401No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG7580.11834966235996722No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7400.115539248214216No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7390.11538311409500762No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7390.11538311409500762No Hit
CCATCCTCCCTGTCTGCTTCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAATCAA7360.11491471173738241No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7350.11475857761817403No Hit
CATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACTCTCACCATCACCGGTCTGCAACCGGAGGATTG7290.1138217729029236No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7290.1138217729029236No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGC7270.11350950466450681No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7220.11272883406846482No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7190.1122604317108396No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG7120.11116749287638081No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7110.11101135875717241No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7100.110855224637964No Hit
CTATCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC7080.11054295639954721No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC6960.10866934696904641No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6950.108513212849838No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCT6930.10820094461142121No Hit
GCCCCTAACCTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGA6930.10820094461142121No Hit
AGTCAGACCATTAGCAATCAATTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTA6900.10773254225379603No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT6840.1067957375385456No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT6760.10554666458487841No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6730.1050782622272532No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6690.1044537257504196No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6580.1027362504391272No Hit
CTCTTATATCGACTACAACTCGTCCTTCCAAGGCCACGTCACCATCTCAACTGACAAGTCCATCAATACTGCCTA6560.1024239822007104No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT6550.10226784808150201No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT6480.10117490924704321No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG6440.10055037277020962No Hit
ATCTGGGACAGATTTCACTCTCACCATCACCGGTCTGCAACCGGAGGATTGTGGATTTTACTTCTGTCAACAGA6440.10055037277020962No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG6440.10055037277020962No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6440.10055037277020962No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6420.10023810453179281No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)