FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004685650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814117
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG33360.4097691118107103No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAT30970.38041215206168155No Hit
GACTTAGGACGGTGACCTTGGTCCCATTTCCGAAGATGTATCTGTGCACT29280.3596534650424939No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC28660.35203785205320615No Hit
ACTTATTACTGCCAGTCGTTTGACTCCACAGTGCACAGATACATCTTCGG27630.33938610789358287No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCATAGAGGTCAGCTTCAAAGAGAACTGGCTCTTGGAGGTGTCAATTGA27430.3369294585421997No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG26800.32919101308534277No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC22030.2705999260548545No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC21710.2666692870926415No Hit
CTCCTGCACTGGCAGCGACTCGAACTTCGGGGCTGGTTATCTTGTACACTGGTACCAGCAATTTCCGGGAACACC20610.25315771566003414No Hit
CTTGTACACTGGTACCAGCAATTTCCGGGAACACCCCCCCGGGTAGTCATATTTAATGACAGAAATAGGCCCTCA19190.2357155052652137No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19170.2354698403300754No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC18950.23276752604355394No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG18720.22994237928946332No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18630.2288368870813409No Hit
TCTGTGCACTGTGGAGTCAAACGACTGGCAGTAATAAGTAGCCTCATCGC18020.22134410655962225No Hit
GTGCACAGATACATCTTCGGAAATGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCAC17720.2176591325325475No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCATTTCCGAAGATGTATCTGTGCA17700.2174134675974092No Hit
GTCTATTACTGTGCACAATTTTCCGGCTACGGCCAGTACCAATTCTCCTACTTGGACGTCTGGGGCAGGGGGG17130.2104120169459672No Hit
TCACTGGACTCCAGCCTGGCGATGAGGCTACTTATTACTGCCAGTCGTTTGACTCCACAGTGCACAGATACATCT16700.20513022084049345No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT16580.20365623122966356No Hit
GGACAATCTCCAGCATGGCCTGGCCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAG16310.2003397546052963No Hit
GTAATAAGTAGCCTCATCGCCAGGCTGGAGTCCAGTGACGGCCAGGGAGG16010.19665478057822156No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15990.19640911564308325No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG15300.18793367538081135No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14910.1831432091456142No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC14340.1761417584941722No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC14030.17233395199952833No Hit
GTGTCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTGCACTGGCAGCGACTCGAACTTCGGGGCTGGTTATCT13690.16815764810217695No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT13110.1610333649831658No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCATTTCCGAAGATGTATCTGTGCACTGTGGAGTCAAACGA12860.15796255329393688No Hit
GATACATCTTCGGAAATGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCC12610.15489174160470792No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGCTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12240.1503469403046491No Hit
ATCTTGTACACTGGTACCAGCAATTTCCGGGAACACCCCCCCGGGTAGTCATATTTAATGACAGAAATAGGCCC12110.14875011822625003No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG12040.14789029095326592No Hit
GGTTATCTTGTACACTGGTACCAGCAATTTCCGGGAACACCCCCCCGGGTAGTCATATTTAATGACAGAAATAGG11950.1467847987451435No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11640.14297699225049962No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11640.14297699225049962No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11360.13953768315856319No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCACCATCTCC11330.13916918575585574No Hit
GTCCAGTGACGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAATCGGTCAGGGACCCATGAGGGCCTAT11230.13794086108016415No Hit
CAGTAATAGACGGCCGTGTCTGCGGCGGTCATAGAGGTCAGCTTCAAAGAGAACTGGCTCTTGGAGGTGTCAAT11180.13732669874231837No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11010.13523854679364267No Hit
GTCACCATCTCCTGCACTGGCAGCGACTCGAACTTCGGGGCTGGTTATCT10930.1342558870530894No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10830.13302756237739782No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10780.13241340003955204No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG10480.1287284260124773No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGGAGGTGGAGT10290.12639460912866332No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG10100.12406079224484934No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9990.12270963510158858No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9950.12221830523131197No Hit
GTCATGGACCTCCTGTGCAAGAACACGAGGCACCTCTGCTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC9670.11877899613937555No Hit
GTGCAGGAGATGGTGACCGTCTGCCCTGGGGCCCCAGACACTGAGGGCGG9620.11816483380152976No Hit
ACCTACTACAACCCGGCCCTCGAGAGTCGAGCCTCCATGTCAATTGACACCTCCAAGAGCCAGTTCTCTTTGAAG9570.11755067146368398No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG9440.11595384938528491No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGCTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG9390.11533968704743912No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9340.11472552470959334No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9310.11435702730688588No Hit
GTAGTAGGTGGTCCCAGTATGATAAATAGACCCAATCCACTCCAGTCCCCTCTCTGGGGACTGCCGGATCCAGGC9260.11374286496904007No Hit
TAAGTAGCCTCATCGCCAGGCTGGAGTCCAGTGACGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAA9240.11349720003390176No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9120.11202321042307188No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA9100.11177754548793355No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9060.11128621561765692No Hit
GTCATATTTAATGACAGAAATAGGCCCTCATGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGC8960.11005789094196533No Hit
GTGCCAGACCTGGAGCCAGAGAATCGGTCAGGGACCCATGAGGGCCTATT8950.10993505847439618No Hit
AAATAGGCCCTCATGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGCCGTCAC8950.10993505847439618No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGCTGCTCTGTTTGGAGGGTT8920.10956656107168872No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG8920.10956656107168872No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGT8870.10895239873384294No Hit
CTCCAGCATGGCCTGGCCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTGT8850.1087067337987046No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8820.10833823639599714No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8820.10833823639599714No Hit
CATGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGCCGTCACTGGACTCCAGCC8800.10809257146085882No Hit
GTGGAGTCAAACGACTGGCAGTAATAAGTAGCCTCATCGCCAGGCTGGAG8720.10710991172030555No Hit
GTGTACAAGATAACCAGCCCCGAAGTTCGAGTCGCTGCCAGTGCAGGAGA8720.10710991172030555No Hit
GGGCAGACGGTCACCATCTCCTGCACTGGCAGCGACTCGAACTTCGGGGCTGGTTATCTTGTACACTGGTACCAG8680.10661858185002894No Hit
CTGTGGAGTCAAACGACTGGCAGTAATAAGTAGCCTCATCGCCAGGCTGG8520.10465326236892239No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC8470.10403910003107661No Hit
GCTTCAGCTGTGGACACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGCCTCCTCTCCTCCTCACTC8450.1037934350959383No Hit
GGCTACTTATTACTGCCAGTCGTTTGACTCCACAGTGCACAGATACATCTTCGGAAATGGGACCAAGGTCACCG8430.1035477701608No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGCCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC8420.10342493769323081No Hit
GTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGCCGTCACTGGACTCCAGCCTGGCGA8400.10317927275809251No Hit
CCACCAAACCCTCCAAACAGAGCAGCAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAG8390.10305644029052335No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG8370.10281077535538503No Hit
GTCAGGGACCCATGAGGGCCTATTTCTGTCATTAAATATGACTACCCGGGGGGGTGTTCCCGGAAATTGCTGGTA8340.10244227795267757No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT8310.10207378054997009No Hit
CAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGCCTCCTCTCCT8260.10145961821212429No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG8250.10133678574455515No Hit
GTGCACTGTGGAGTCAAACGACTGGCAGTAATAAGTAGCCTCATCGCCAG8210.10084545587427851No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGCCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGG8150.10010846106886356No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTGCACTGGCAGCGACTCGAACTTCGGGGCTGGT8150.10010846106886356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)