FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814117
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT31030.3811491468670965No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCATAGAGGTCAGCTTCAAAGAGAACTGGCTCTTGGAGGTGTCAATT30660.37660434556703765No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC29560.3630927741344303No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA27500.3377892858151838No Hit
ACTTATTACTGCCAGTCGTTTGACTCCACAGTGCACAGATACATCTTCGG25030.30744966632560183No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCACCATCTCCT23560.28939329359293564No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG23490.2885334663199515No Hit
GACTTAGGACGGTGACCTTGGTCCCATTTCCGAAGATGTATCTGTGCACT21070.25880800916821534No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTGCACTGGCAGCGACTC20990.2578253494276621No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC20660.2537718779978799No Hit
CTCCTGCACTGGCAGCGACTCGAACTTCGGGGCTGGTTATCTTGTACACTGGTACCAGCAATTTCCGGGAACACC19180.23559267279764456No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18460.22674873513266522No Hit
CTTGTACACTGGTACCAGCAATTTCCGGGAACACCCCCCCGGGTAGTCAT18400.22601174032725024No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG18120.22257243123531384No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18030.2214669390271914No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17500.21495681824602605No Hit
GTCTATTACTGTGCACAATTTTCCGGCTACGGCCAGTACCAATTCTCCTACTTGGACGTCTGGGGCAGGGGGGT16910.20770970265944574No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA16840.20684987538646163No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT16740.20562155071077007No Hit
GTGCACAGATACATCTTCGGAAATGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACT16530.20304206889181775No Hit
TCACTGGACTCCAGCCTGGCGATGAGGCTACTTATTACTGCCAGTCGTTT16470.20230507408640283No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16130.19812877018905145No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16040.19702327798092903No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCATTTCCGAAGATGTATCTGTGCA15560.19112731953760947No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT14810.18191488446992263No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG14610.17945823511853948No Hit
GGACAATCTCCAGCATGGCCTGGCCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT14440.1773700831698638No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13970.17159695719411336No Hit
TCTGTGCACTGTGGAGTCAAACGACTGGCAGTAATAAGTAGCCTCATCGCCAGGCTGGAGTCCAGTGACGGCCAG13890.17061429745356013No Hit
GTGTCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTGCACTGGCAGCGACTCGAACTTCGGGGCTGGTTATCTT12700.15599723381283034No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCATTTCCGAAGATGTATCTGTGCACTGTGGAGTCAAAC12700.15599723381283034No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG12590.1546460766695696No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT12500.15354058446144717No Hit
GTAATAAGTAGCCTCATCGCCAGGCTGGAGTCCAGTGACGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGA12190.1497327779668033No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT12110.14875011822625003No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12060.14813595588840422No Hit
ATCTTGTACACTGGTACCAGCAATTTCCGGGAACACCCCCCCGGGTAGTCATATTTAATGACAGAAATAGGCCCT12010.14752179355055844No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGCTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT11910.14629346887486688No Hit
GTGTACAAGATAACCAGCCCCGAAGTTCGAGTCGCTGCCAGTGCAGGAGA11460.14076600783425477No Hit
CAGTAATAGACGGCCGTGTCTGCGGCGGTCATAGAGGTCAGCTTCAAAGAGAACTGGCTCTTGGAGGTGTCAATT11440.14052034289911647No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCAC11390.1399061805612707No Hit
GGTTATCTTGTACACTGGTACCAGCAATTTCCGGGAACACCCCCCCGGGTAGTCATATTTAATGACAGAAATAG11190.13744953120988754No Hit
GATACATCTTCGGAAATGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCC11090.13622120653419595No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG10650.130816577961153No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCT10310.12664027406380166No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAG10300.1265174415962325No Hit
GTCCAGTGACGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAAT10180.1250434519854026No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC10080.123815127309711No Hit
GTCACCATCTCCTGCACTGGCAGCGACTCGAACTTCGGGGCTGGTTATCTTGTACACTGGTACCAGCAATTTCCG10020.12307813250429607No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9900.12160414289346619No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9850.12098998055562038No Hit
GTCATGGACCTCCTGTGCAAGAACACGAGGCACCTCTGCTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC9770.12000732081506713No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9640.11841049873666806No Hit
GACTTAGGACGGTGACCTTGGTCCCATTTCCGAAGATGTATCTGTGCACTGTGGAGTCAAACGACTGGCAGTAAT9640.11841049873666806No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAG9540.11718217406097649No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG9330.11460269224202418No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACT9310.11435702730688588No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG9240.11349720003390176No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9190.11288303769605597No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC9150.11239170782577934No Hit
GTAGTAGGTGGTCCCAGTATGATAAATAGACCCAATCCACTCCAGTCCCCTCTCTGGGGACTGCCGGATCCAGGC9090.1116547130203644No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGCTGCTCTGTTTGGAGGGTTTG8810.10821540392842798No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAG8770.10772407405815136No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG8760.10760124159058218No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGCTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG8650.10625008444732145No Hit
GGGCAGACGGTCACCATCTCCTGCACTGGCAGCGACTCGAACTTCGGGGCTGGTTATCTTGTACACTGGTACCA8620.10588158704461396No Hit
GTGCAGGAGATGGTGACCGTCTGCCCTGGGGCCCCAGACACTGAGGGCGGCTGCGTCAACACAGACTGGGCCCAG8590.1055130896419065No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8530.10477609483649157No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC8420.10342493769323081No Hit
GTCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTGCACTGGCAGCGACT8400.10317927275809251No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8310.10207378054997009No Hit
GGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCACCATCTC8290.10182811561483178No Hit
AAATAGGCCCTCATGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGCCGTCACTG8280.10170528314726263No Hit
GTCATATTTAATGACAGAAATAGGCCCTCATGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGC8270.10158245067969346No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8270.10158245067969346No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC8240.10121395327698599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA2900.091.9545170
CCTAGAA700.00405297760.15069670
TAATTAA1301.3224893E-553.9813970
AAGCGTG200.00777834551.028337
AGGGACA850.00873685549.53586670
GTGGTAT1950.048.8956871
TAGGGAA3750.044.9125270
AAGCGGT5350.044.59771370
GCAAAGT1902.3940083E-644.32156870
TCCCATT22100.043.18511270
GCACCGT8550.041.03848670
CTCGTAT1755.7163274E-540.10046470
GGTATCA6550.040.031011
GAGCCAT6700.039.80120570
GTGTAAA350.001342641939.45799650
TTTAAGT1500.001737502937.427170
GTATCAA15100.037.209531
ACCTGGT2706.3819243E-736.3874670
GTCCTAA21250.036.326370
TGGTATC2550.036.0266382
Produced by FastQC (version 0.11.5)