FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638642
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT29770.4661453521691339No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG25320.3964662518280977No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT21460.3360255041165473No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20740.3247515822636156No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA19320.30251690305366696No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC18310.28670209600997115No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG17910.28043880609167576No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGA16820.26337134106432086No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG16320.2555422286664516No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15650.24505121805330687No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14820.23205489147284392No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAGGTGTCCCCCTTCTTCCAG14190.2221902098515287No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14050.21999805838012532No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13660.21389135070978732No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGG13610.2131084394700004No Hit
GGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG12300.19259616498758303No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT11820.18508021708562855No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11580.18132224313465134No Hit
GTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGC11240.17599844670410028No Hit
GGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGGTGGTGCC11210.17552869996022813No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11140.17443262422452643No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10920.17098781476946395No Hit
GCCCCAAGGACGTGCTGGTTCGCTGGCTGCAGGGGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCA10740.16816933430623102No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC10660.16691667632257196No Hit
CCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCG10200.15971389291653226No Hit
ATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGA9710.15204136276662042No Hit
GTCAGGTACTTCTCGCGGGGCAGCTCCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCG9500.14875313555951533No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9470.14828338881564318No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9150.14327275688100688No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8780.13747921370658367No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8730.13669630246679673No Hit
GTATATTACTGTGCGAGAGAGTTCCCACAGATTTTGGAGTGGTTACCCAC8730.13669630246679673No Hit
GTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAGGTGTCCCCCTTCT8310.13011984805258658No Hit
GGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAA8300.1299632658046292No Hit
GGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG7960.12463946937407812No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7880.12338681139041904No Hit
GCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGC7630.11947225519148444No Hit
CAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGCGGGGCAGCTC7590.11884592619965491No Hit
CCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAG7530.11790643271191059No Hit
GTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTG7460.11681035697620888No Hit
GACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGTGGCAGC7350.11508795224867766No Hit
GCATGGAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGT7210.11289580077727428No Hit
GGGCAGCTCCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAG7060.1105470670579135No Hit
GACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGC6970.10913782682629705No Hit
GTCCAGTGTCCTGCCGGGCTGTGCCGAGCCATGGAACCATGGGAAGACCT6790.10631934636306413No Hit
CCATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGG6780.10616276411510675No Hit
GGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTC6770.10600618186714936No Hit
GGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGCGGGGCAGCTCCTGTGACCCCTGCAGCCAGCGAA6750.1056930173712346No Hit
GGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGC6710.10506668837940505No Hit
GGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAG6640.10397061264370336No Hit
GACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCAC6620.1036574481477886No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT6520.10209162566821475No Hit
GTGCCGAGCCATGGAACCATGGGAAGACCTTCACTTGCACTGCTGCCTACCCCGAGTCCAAGACCCCGCTAACCG6460.10115213218047045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)