FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093572
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTATGGTACTTCACAGATCACCTTCGGCCAAGGGACACGACTGGAGAATAGACGAACTGT48390.4424948700222756No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44000.40235119406861186No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA42500.3886346760890001No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35830.32764189280632644No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30450.27844531498611885No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT30120.27542768103060433No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA28870.26399724938092783No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC27850.2546700171547918No Hit
GTCTATTACTGTGCGAGAGACTTTGGATATGATTGTCCTGGTGGAATATGTGATCGGTACGACTACTTCTATATG27810.2543042433420022No Hit
GTAATAGACAGCCGTGTCTTCAGATCTCAGGTTGCTCATCTCCAAATAGG26990.2468058801798144No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA25380.2320834842150311No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23220.21233169832439017No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC22840.2088568471028885No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC22820.2086739601964937No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA22720.20775952566451958No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22480.20556488278778168No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22100.20209003156628005No Hit
GTGTATTACTGTCAGCACTATGGTACTTCACAGATCACCTTCGGCCAAGGGACACGACTGGAGAATAGACGAA20670.18901361775905015No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA20300.18563020999074592No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA19870.18169814150325722No Hit
GTACCATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC19270.1762115343114125No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19030.1740168914346746No Hit
CTATGGTACTTCACAGATCACCTTCGGCCAAGGGACACGACTGGAGAATAGACGAACTGTGGCTGCACCAT18860.17246235273031862No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCACTAGAAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAG18180.16624419791289463No Hit
GTATTAGCACTAGAAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCT18130.16578698064690758No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17330.15847150439111463No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT16570.15152180194811132No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16280.1488699418053864No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16040.1466752989286485No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT15540.1421031262687779No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15280.13972559648564523No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA15220.13917693576646073No Hit
GAGTATTAGCACTAGAAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG15070.13780528396849956No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15000.13716517979611767No Hit
GTCCCAGACCCACTGCCACTGAACGTGTCTGGGATGCCTATGGCCCTACTGAATGCATCATAGATGACGAGCCTG14960.13679940598332802No Hit
GTAATAGACAGCCGTGTCTTCAGATCTCAGGTTGCTCATCTCCAAATAGGCAGTGGTCGCGGAGGTGTCCCTGGT14180.1296668166339299No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14060.12856949519556096No Hit
CAGTAGGGCCATAGGCATCCCAGACACGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCA13940.12747217375719203No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT13210.12079680167378097No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13170.12043102786099133No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13160.12033958440779392No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13050.11933370642262238No Hit
TCCCAGACACGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACC13040.11924226296942497No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA12970.11860215879704308No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCACTAGAAACTTAGCCTG12780.11686473318629226No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT12480.11412142959036992No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA12380.11320699505839579No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA12370.11311555160519837No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12200.11156101290084237No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG12090.11055513491567084No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGAA11970.10945781347730191No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC11910.10890915275811744No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC11870.1085433789453278No Hit
GTGAAGTACCATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT11850.10836049203893296No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11800.10790327477294591No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG11750.10744605750695885No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11630.1063487360685899No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11630.1063487360685899No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11620.10625729261539249No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11610.10616584916219508No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11490.10506852772382615No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCACTATGGTACTTCA11450.10470275391103649No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11260.10296532830028568No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11250.10287388484708825No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACGTGTCTGGGATGCCTATGGCCCTACTGAATGCATCATA11000.10058779851715297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT455.710599E-498.5672570
GGCGTGT508.6803833E-488.71052670
ACGGATT150.002514178667.8748629
CGTGCGT700.0032988463.36466270
GATGACA800.005597383755.44407770
GTTATTA800.005597383755.44407770
GTATTAA1255.4861134E-447.31228370
CCCTGGT11100.046.99958469
GTGGTAT3200.046.5742761
GTAGTTT1306.655971E-445.49257770
ATGCATT14450.045.0203470
GTCACTA1004.930507E-444.35526370
TGGTATC3400.043.8025442
AAGCGGT11600.043.335670
GAGCAAA23550.041.43591370
GGTATCA10350.041.235721
TTAACGA350.001344575939.4476548
GTATCAA26150.039.1181871
GTTTGGT1909.154577E-538.90812770
TCCCTGG10650.036.20033368
Produced by FastQC (version 0.11.5)