FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617888
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT28320.4583354912217101No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24390.39473173131700245No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21640.3502252835465327No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18960.3068517271738568No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT18780.3039385778652442No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA17850.2888873064374126No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16060.2599176549795432No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15190.24583743332124916No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAC15110.24454270029519912No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTA14930.2416295509865866No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC14590.23612693562587395No Hit
TTATTATTGTCAGCAGCGTGGCAGCTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTG14490.2345085193433114No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTCTGCACTTTATTATTGTCAGCAGCG14270.23094800352167383No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14100.22819669584131752No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13670.22123750582629864No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13590.2199427728002486No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGC13410.21702962349163601No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13300.21524936558081723No Hit
GTCAGAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGA12940.2094230669635921No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC12770.20667175928323578No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12770.20667175928323578No Hit
GAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC12690.2053770262571858No Hit
GTCTCTGGTGGCTCCATCAATGGTTACTACTGGAACTGGATCCGGCAGACCGCCGGGAAGGGACCGGAGTACAT12560.20327308508985448No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGC12410.20084546066601064No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12370.20019809415298567No Hit
GTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC12320.1993888860117044No Hit
GCCTAGAGCCTGAAGATTCTGCACTTTATTATTGTCAGCAGCGTGGCAGCTGGCCTCGGACGTTCGGCCAAGGGA12310.19922704438344813No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGCCAG12250.19825599461391058No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA12230.1979323113573981No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT12140.19647573670309185No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTCTGCACTTTATTATTGTCAGCAGCGTGGCAGC11610.18789813040551037No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC11480.18579418923817909No Hit
CAATAATAAAGTGCAGAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG11260.1822336734165415No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10380.1679916101299912No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA9760.15795742917810346No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCAC9760.15795742917810346No Hit
GTGTATTACTGTGCGAGAGATACCCCTAGCGATTCGGGGACTTATTATGTTGACTACTGGGGCCAGGGAACCC9720.15731006266507847No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9670.1565008545237972No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9650.1561771712672847No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9420.15245481381739084No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGT9310.15067455590657206No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9120.14759956496970325No Hit
CAGTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGAACTGGTTCTTGGACGTGTCTA9080.14695219845667823No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8650.13999300844165932No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCGCTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGA8630.13966932518514683No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8600.13918380030037808No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8340.13497591796571548No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8280.13400486819617796No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8220.13303381842664044No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8150.13190092702884665No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTC8150.13190092702884665No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8070.1306061940027966No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGGTGGTAACACTCTGAC7930.12834041120720907No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCA7900.12785488632244033No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7880.1275312030659278No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7750.12542726189859651No Hit
GTCCCAGACCCACTGCCGCTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCC7540.12202858770521519No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG7480.12105753793567767No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGG7470.1208956963074214No Hit
GGTTTCTGTTGGTACCAGGCTAAGTAGGTGGTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCCC7360.11911543839660262No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAATGGTTACTACTGGAACTGGA7290.11798254699880883No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7240.11717333885752758No Hit
CCCAGATACCACCGGAGAAGTTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCAC7230.11701149722927132No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG7210.1166878139727588No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7200.11652597234450256No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGGTGGTA7190.11636413071624632No Hit
CACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCCCCTGGAGACAAA7150.1157167642032213No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7140.11555492257496504No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7120.11523123931845253No Hit
CTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC7070.11442203117717127No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT6940.11231809000983997No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCT6940.11231809000983997No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG6940.11231809000983997No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC6920.11199440675332746No Hit
GTATTACTGTGCGAGAGATACCCCTAGCGATTCGGGGACTTATTATGTTGACTACTGGGGCCAGGGAACCCTGG6920.11199440675332746No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTCTCCAGCCACCCTGTCT6910.11183256512507121No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6910.11183256512507121No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT6900.11167072349681495No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTA6830.11053783209902118No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT6700.1084338909316899No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGT6680.10811020767517737No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTC6660.10778652441866486No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT6610.1069773162773836No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTCTC6610.1069773162773836No Hit
CTGCAGGAGAGGGTGGCTCCTTCCCCTGGAGACAAAGACAGGGTGGCTGG6610.1069773162773836No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG6600.10681547464912736No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6590.1066536330208711No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC6570.10632994976435858No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT6530.10568258325133358No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6530.10568258325133358No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG6520.10552074162307733No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6490.10503521673830855No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT6380.10325495882748978No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC6370.10309311719923352No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6340.10260759231446476No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6290.10179838417318349No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6220.10066549277538972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAT150.002523624367.8071751
CGGCAAA701.452928E-461.91089670
GTATAAT200.00788254350.8553771
CGTGGGA200.00789264350.83891323
TCGTATG1250.046.54614640
GGTATCA3750.046.108881
ACGAGAC1550.045.91530222
CGTATGC1300.044.79252641
CGAGACA1600.044.48404723
ATCTCGT1250.043.69383237
CTTATAC1650.043.150021
TATTCTA1050.001074104541.27393370
GTATGCC1500.041.14412742
GAGCAAA8000.040.62902570
GATCTCG1350.040.4144136
TTATACA1800.039.5253752
TACACAT1800.039.5253755
AGTAAGA1100.001350203839.39784270
GCTTGGG702.5496774E-838.7469561
CCTATCC350.001473172838.7187353
Produced by FastQC (version 0.11.5)