FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617888
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29350.47500517893210414No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA22330.3613923558962142No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18520.2997306955305816No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGAACTGGTTCTTGGACGTGTCTACT18290.2960083380806877No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18250.2953609715676628No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17540.2838702159614688No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT16910.27367419338132476No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC16700.27027551918794346No Hit
TTATTATTGTCAGCAGCGTGGCAGCTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGT14540.2353177274845927No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14400.2330519446890051No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAC14000.22657827955875498No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC13950.22576907141747374No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTA13870.22447433839142367No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT13800.2233414469936299No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC13380.21654409860686727No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA13350.21605857372209852No Hit
GTCCCAGACCCACTGCCGCTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCCTG13090.2118506913874359No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12810.20731912579626083No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCGCTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATA12730.20602439277021076No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT12680.20521518462892951No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12670.20505334300067327No Hit
GCCTAGAGCCTGAAGATTCTGCACTTTATTATTGTCAGCAGCGTGGCAGCTGGCCTCGGACGTTCGGCCAAGG12500.20230203532031693No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGCCA12290.19890336112693563No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12220.19777046972914183No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT12120.19615205344657932No Hit
GTCTCTGGTGGCTCCATCAATGGTTACTACTGGAACTGGATCCGGCAGACCGCCGGGAAGGGACCGGAGTACATT11980.19388627065099176No Hit
CAATAATAAAGTGCAGAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC11730.1898402299445854No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC11420.18482313946864157No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTCTGCACTTTATTATTGTCAGCAGCGTGGCAGCTG11190.18110078201874774No Hit
GTCAGAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGAT10830.1752744834015226No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA10620.17187580920814127No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA10470.16944818478429746No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10360.1676679268734787No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA10250.16588766896265988No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10170.16459293593660987No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCA10050.16265083639753483No Hit
GAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC9910.16038505360194727No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9700.15698637940856597No Hit
GTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC9410.1522929721891346No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA9140.14792324822621575No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9020.1459811486871407No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8800.14242063286550313No Hit
GTGTATTACTGTGCGAGAGATACCCCTAGCGATTCGGGGACTTATTATGTTGACTACTGGGGCCAGGGAACCCTG8650.13999300844165932No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACT8550.13837459215909678No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCA8390.13578512610699672No Hit
CAGTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGAACTGGTTCTTGGACGTGTCTA8240.1333575016831529No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8170.13222461028535915No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7940.12850225283546532No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG7850.12704567818115903No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7850.12704567818115903No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT7570.12251411258998395No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7540.12202858770521519No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA7400.11976280490962764No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7350.11895359676834638No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7290.11798254699880883No Hit
TCCGAGGCCAGCTGCCACGCTGCTGACAATAATAAAGTGCAGAATCTTCA7180.11620228908799006No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT7060.11426018954891501No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGGTGGTAACACTCTGACTG7050.11409834792065876No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC7030.11377466466414626No Hit
CACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCCCCTGGAGACAAA7020.11361282303589No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCA7020.11361282303589No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTT7000.11328913977937749No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGGTGGTAACACTCTGACTGGCCCTGCAGGAGA6980.11296545652286498No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6960.11264177326635247No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC6900.11167072349681495No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA6860.11102335698378996No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAATGGTTACTACTGGAACTGGATCCGGCAGACCGCCGGGAAGGGACC6860.11102335698378996No Hit
GTATTACTGTGCGAGAGATACCCCTAGCGATTCGGGGACTTATTATGTTGACTACTGGGGCCAGGGAACCCTGGT6840.11069967372727743No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTG6830.11053783209902118No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC6810.11021414884250867No Hit
CTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC6760.1094049407012274No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCA6730.10891941581645864No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT6670.10794836604692112No Hit
GGTTTCTGTTGGTACCAGGCTAAGTAGGTGGTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCCC6540.10584442487958984No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG6490.10503521673830855No Hit
CCCAGATACCACCGGAGAAGTTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCAC6420.10390232534051479No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC6390.10341680045574603No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6380.10325495882748978No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC6370.10309311719923352No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG6360.10293127557097727No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA6350.102769433942721No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6320.10228390905795223No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTCTC6300.10196022580143975No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTG6250.10115101766015847No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGT6240.10098917603190223No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6240.10098917603190223No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6210.10050365114713346No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTA6190.10017996789062095No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6180.1000181262623647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)