FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041048
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGTACTGGTCGTCTATGATGATACCGACCGGCCCTCAGGGATCCCT57110.5485818137107992No Hit
GTACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTAAACTT50950.48941067078559297No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCACTGGCCCCAGGACAGACGGCCAGAATTACCTGTGGGGGAAACA46560.4472416257463633No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCACATGATCACTACTATCATCCCACAC38570.3704920426339612No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC36770.353201773597375No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCACATGATCACTACTATCATCCCACACCTGACAGTAAT36490.3505121761916838No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA35160.3377365885146506No Hit
GTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCACTGGCCCCAGGACAGACGGCCAGAATTAC28980.278373331489038No Hit
CCCCAGGACAGACGGCCAGAATTACCTGTGGGGGAAACAACATTGGAAGTTTAAGTGTGCACTGGTACCAGCAGA25040.2405268537089548No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG24310.23351468904411707No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24240.2328422896926943No Hit
GAATTACCTGTGGGGGAAACAACATTGGAAGTTTAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC23990.2304408634376129No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC22980.22073910136708397No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22970.2206430443168807No Hit
GAGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCACTGGCCCCAGGACAGACGGCCAGAATTAC22480.2159362488569211No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21480.20633054383659544No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC21410.2056581444851726No Hit
CTACTATCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCCTC20720.1990302080211479No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT20400.1959563824146437No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20230.19432341256118835No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20200.19403524141057857No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20000.19211410040651344No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19900.19115352990448087No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTGCTGACT19880.19096141580407436No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGAGTCTGTG19830.19048113055305807No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC19560.18788759019757015No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19140.18385319408903336No Hit
GAAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATAAAGCGTGTTCTTGGAAATGTCTCTGG19130.1837571370388301No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC19050.18298868063720405No Hit
CCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCACTGGCCCCAGGAC18960.18212416718537475No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTGCTGACTCAGCC18780.1803951402817161No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC18690.17953062682988682No Hit
GCACAGAGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA17920.17213423396423602No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCACATGATCACTACTATCATCCCACACCTGACAGTAAT17830.17126972051240671No Hit
GTACTGGTCGTCTATGATGATACCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAAC17310.16627475390183738No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG17240.16560235455041458No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAT17240.16560235455041458No Hit
TGATGATACCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGAC17210.1653141833998048No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17180.16502601224919503No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC17010.16339304239573968No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16920.16252852894391037No Hit
GTATCATCATAGACGACCAGTACAGGGGCCTGGCCTGGCTTCTGCTGGTA16750.16089555909045503No Hit
CTCACTGCACAGAGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCACTGGCCCCAGGACAGACGG16350.15705327708232475No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTA16300.15657299183130846No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGGTATCATCAT16200.15561242132927589No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT16100.15465185082724334No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16070.15436367967663356No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG15610.14994505536728375No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG15520.14908054191545442No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15030.14437374645549483No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCG14960.14370134710407206No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAGGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATGATAGT14680.14101174969838087No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14630.14053146444736458No Hit
CTATTACTGTCAGGTGTGGGATGATAGTAGTGATCATGTGCTATTCGGCG14590.14014723624655154No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14460.1388984945939092No Hit
CAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCC14220.13659312538903107No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13860.1331350715817138No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13710.13169421582866497No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG13500.12967701777439655No Hit
GTTTAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTACTGGTCGTCTATGATGATACCGACCGGC13110.12593079281646957No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATG13100.1258347357662663No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13040.12525839346504675No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12980.12468205116382722No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12800.1229530242601686No Hit
CTATGTAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACATTTCCAAGAACACGCTTTATCTGCAAATGA12590.12093582620590021No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC12560.12064765505529045No Hit
GGCCAGGCCCCTGTACTGGTCGTCTATGATGATACCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCC12380.11891862815163182No Hit
GTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAG12340.1185343999508188No Hit
GTGCTGACTCAGCCACCCTCGGTGTCACTGGCCCCAGGACAGACGGCCAG12210.11728565829817646No Hit
GTGTATTTCTGTGCGAGAGAGGATACGGTGACTACGAACTTTGACTGCTGGGGCCAGGGAACCCTGGTCACCGT12170.11690143009736341No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTGCTGACTCAGC12120.11642114484634715No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA12100.11622903074594063No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12010.11536451729411133No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12000.11526846024390805No Hit
CCCTCGGTGTCACTGGCCCCAGGACAGACGGCCAGAATTACCTGTGGGGGAAACAACATTGGAAGTTTAAGTGTG11990.11517240319370481No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCACATGATC11970.11498028909329829No Hit
TGATAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAGGCTGACCGTCC11930.11459606089248527No Hit
CTGCACAGAGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCACTGGCCCCAGGACAGACGGCC11870.11401971859126571No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCC11840.11373154744065594No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11790.11325126218963968No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCC11610.11152223528598106No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11550.11094589298476151No Hit
GCCTGGTCCCTCCGCCGAATAGCACATGATCACTACTATCATCCCACACCTGACAGTAATAGTCGGCCTCATCCC11360.10912080903089964No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11320.1087365808300866No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11310.10864052377988336No Hit
CTATCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCCTCGAC11280.10835235262927358No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11270.10825629557907031No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11160.10719966802683449No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG10930.10499035587215959No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA10900.10470218472154982No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGAGTCTGTGACCTC10760.10335738601870423No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10730.10306921486809446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)