FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041048
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGTACTGGTCGTCTATGATGATACCGACCGGCCCTCAGGGATCCCT55930.5372470817868148No Hit
GTACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTAAACTT49070.47135194534738073No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCACTGGCCCCAGGACAGACGGCCAGAATTACCTGTGGGGGAAACA43100.41400588637603647No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCACATGATCACTACTATCATCCCACAC34920.3354312193097725No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA33800.3246728296870077No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC33520.32198323228131653No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCACATGATCACTACTATCATCCCACACCTGACAGTAAT31880.3062298760479824No Hit
GTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCACTGGCCCCAGGACAGACGGCCAGAATTAC29750.2857697243546887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27390.26310026050672014No Hit
CCCCAGGACAGACGGCCAGAATTACCTGTGGGGGAAACAACATTGGAAGTTTAAGTGTGCACTGGTACCAGCAGA22810.21910613151362857No Hit
GAGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCACTGGCCCCAGGACAGACGGCCAGAATTAC22690.2179534469111895No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC22080.21209396684879084No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG22000.2113255104471648No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21730.20873197009167685No Hit
GAATTACCTGTGGGGGAAACAACATTGGAAGTTTAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC21720.20863591304147358No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21550.20700294318801823No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC21200.20364094643090425No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTGCTGACT20910.2008552919750098No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT20640.1982617516195219No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGAGTCTGTG20580.19768540931830234No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAT20160.19365101320976555No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC19820.1903850735028548No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19810.19028901645265156No Hit
CTACTATCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCCTC19420.18654279149472452No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC19060.1830847376874073No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18650.17914639862907378No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18650.17914639862907378No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGGTATCATCAT18590.17857005632785425No Hit
GAAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATAAAGCGTGTTCTTGGAAATGTCTCTGG18150.17434354611891095No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17880.171750005763423No Hit
CCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCACTGGCCCCAGGAC17850.17146183461281325No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTGCTGACTCAGCC17100.164257555847569No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC17030.1635851564961462No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG16900.16233641484350386No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC16660.1600310456386257No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16620.15964681743781267No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16560.15907047513659314No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTA16520.1586862469357801No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT16510.15859018988557685No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16270.15628482068069868No Hit
GTACTGGTCGTCTATGATGATACCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAAC16100.15465185082724334No Hit
TGATGATACCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGAC16070.15436367967663356No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCACATGATCACTACTATCATCCCACACCTGACAGTAAT15850.1522504245721619No Hit
GTATCATCATAGACGACCAGTACAGGGGCCTGGCCTGGCTTCTGCTGGTA15680.15061745471870652No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15610.14994505536728375No Hit
GCACAGAGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA15560.14946477011626746No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15340.1473515150117958No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGCTACTGAAGGTGAATCCAGACGCTGCAC15190.14591065925874694No Hit
CTATTACTGTCAGGTGTGGGATGATAGTAGTGATCATGTGCTATTCGGCG15090.1449500887567144No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG14790.1420683772506167No Hit
CTCACTGCACAGAGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCACTGGCCCCAGGACAGACGG14640.14062752149756783No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14200.13640101128862453No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCG14140.135824668987405No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG13920.13371141388293337No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAGGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATGATAGT13880.13332718568212032No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTGCTGACTCAGC13840.1329429574813073No Hit
CAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCC13510.12977307482459982No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGAGTCTGTGACCTC13320.12794799087073797No Hit
GTGTATTTCTGTGCGAGAGAGGATACGGTGACTACGAACTTTGACTGCTGGGGCCAGGGAACCCTGGTCACCGT13250.12727559151931514No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13230.12708347741890863No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13180.12660319216789237No Hit
TGATAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAGGCTGACCGTCC12710.12208851080833928No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12470.11978314160346112No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATG12330.11843834290061553No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC12260.11776594354919274No Hit
CTGCACAGAGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCACTGGCCCCAGGACAGACGGCC12220.1173817153483797No Hit
GCCTGGTCCCTCCGCCGAATAGCACATGATCACTACTATCATCCCACACCTGACAGTAATAGTCGGCCTCATCCC12210.11728565829817646No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12160.11680537304716017No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCACATGATC12140.11661325894675365No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12130.1165172018965504No Hit
GTTTAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTACTGGTCGTCTATGATGATACCGACCGGC11810.11344337629004618No Hit
GTGCTGACTCAGCCACCCTCGGTGTCACTGGCCCCAGGACAGACGGCCAG11540.11084983593455824No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11530.110753778884355No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11480.11027349363333872No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11440.1098892654325257No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11410.10960109428191592No Hit
GTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAG11320.1087365808300866No Hit
GGCCAGGCCCCTGTACTGGTCGTCTATGATGATACCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCC11230.10787206737825729No Hit
CTATGTAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACATTTCCAAGAACACGCTTTATCTGCAAATGA11180.10739178212724101No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11030.10595092637419216No Hit
CCCTCGGTGTCACTGGCCCCAGGACAGACGGCCAGAATTACCTGTGGGGGAAACAACATTGGAAGTTTAAGTGTG10910.10479824177175308No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10730.10306921486809446No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10620.10201258731585865No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC10550.10134018796443583No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10450.10037961746240326No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA10430.10018750336199676No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCC10420.1000914463117935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAT15100.087.6813370
TCAACGG801.5277596E-461.29574670
ACTCTGG29100.059.8212770
AGGCTCG902.7344687E-454.48510470
TCTCGCG5800.042.27292670
CAGGTAA14750.040.53627469
AAGGTAC350.001296862539.7384555
TGGTATC3000.038.4834942
ACCCGAT1350.00201411836.32340270
GTATCAA19150.036.2124821
ATTCCGG5500.033.43404470
GGCGGCG8900.033.05837670
ACAGGTA14300.032.7683768
TGTTGGG1500.00337644932.69106370
AACACAT6750.032.69106370
TATCAAC21150.032.430812
CCAATGT20050.032.40572770
ATCAACG21850.031.0765483
GAGTTTA2200.030.9032541
TCAACGC22100.030.8860454
Produced by FastQC (version 0.11.5)