FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147285
Sequences flagged as poor quality0
Sequence length19-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATACTCCCCCTCTCTCGCACAGTAATACACGGCCGTGTCCGCAGC53440.46579533420205094No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCATCTTCAGGGAGAATTGGTTCTTGGACGTGTCTCCTGA46200.4026898285953359No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCG41840.36468706555040814No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG38140.3324370143425565No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34080.29704912031448155No Hit
GTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAGGGGACTGGAGTGGAT30390.26488623140719175No Hit
GTATAGTACCAGTCGATTCTATTACCACTATTACATGGACGTCTGGGGCA29850.2601794671768567No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGC28780.25085310101674824No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCGCGAACCACTC26610.23193888179484612No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC26500.23098009648866674No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG24100.2100611443538441No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23020.2006476158931739No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC22630.1972482861712652No Hit
GGTTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCATCATCTTTGGTAACACCAATCGG22210.19358746954767125No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAG21360.18617867399992155No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTT21230.18504556409261866No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21170.18452259028924808No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCATCAT21060.18356380498306873No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20830.1815590720701482No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20240.17641649633700432No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19870.17319149121621916No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19750.17214554360947804No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19640.17118675830329866No Hit
GTGTATTACTGTGCGAGAGAGGGGGAGTATAGTACCAGTCGATTCTATTA19550.1704022975982428No Hit
GTGATGGTCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTGGTG19160.16700296787633412No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19050.16604418257015477No Hit
GATTATTACTGCCAGTCCTATGACATCAGCCTGAGTGGTTCGCGGGTATTCGGCGGAGGGACCAAGCTGACCGTC18720.16316782665161664No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18360.16002998383139325No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCATCTTCAGGGAGAATTGGTTCTTGGACGTGTCTC18180.15846106242128155No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17910.156107680306114No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC17870.15575903110386696No Hit
GACCATCACTGGGCTCCAGTCTGAAGATGAGTCTGATTATTACTGCCAGT17810.1552360573004964No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17440.15201105217971123No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT17420.1518367275785877No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17190.1498319946656672No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17090.14896037166004958No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC17020.1483502355561173No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCAGCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGA16490.14373063362634395No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAGGGGA16040.1398083301010647No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15540.13545021507297664No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG15480.1349272412696061No Hit
GTCCTATGACATCAGCCTGAGTGGTTCGCGGGTATTCGGCGGAGGGACCA15480.1349272412696061No Hit
TCACTGGGCTCCAGTCTGAAGATGAGTCTGATTATTACTGCCAGTCCTATGACATCAGCCTGAGTGGTTCGCGGG15390.13414278056455023No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT15300.1333583198594944No Hit
GGCTGATGTCATAGGACTGGCAGTAATAATCAGACTCATCTTCAGACTGG14830.1292616917330916No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCGCGAACCACTCA14610.12734412112073287No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14610.12734412112073287No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG14500.1263853358145535No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA14490.12629817351399172No Hit
GGGCAGAGGGTCAGCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG14420.1256880374100594No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14260.12429344060107123No Hit
GTGCAGGAGATGCTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG14250.12420627830050947No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14140.12324749299433009No Hit
GTATTACTGTGCGAGAGAGGGGGAGTATAGTACCAGTCGATTCTATTACC14050.12246303228927424No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14020.12220154538758897No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13930.12141708468253311No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG13750.11984816327242141No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13650.11897654026680379No Hit
GTAATAATCAGACTCATCTTCAGACTGGAGCCCAGTGATGGTCAGGGAGG13610.11862789106455676No Hit
CTATTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCACCATCCCCGACCAGCCCCAAGGTCT13520.1178434303595009No Hit
GTCTGAAGATGAGTCTGATTATTACTGCCAGTCCTATGACATCAGCCTGA13490.11758194345781563No Hit
TCTTCAGACTGGAGCCCAGTGATGGTCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC13320.11610018434826568No Hit
GTCTGGCACCTCAGCCTCCCTGACCATCACTGGGCTCCAGTCTGAAGATGAGTCTGATTATTACTGCCAGTCCTA13280.11575153514601864No Hit
GCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCATCATCT13200.11505423674152457No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13180.11487991214040104No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCGCGAACCA13150.11461842523871575No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA13030.11357247763197462No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT12790.11148058241849236No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12730.11095760861512179No Hit
GCTTGGTCCCTCCGCCGAATACCCGCGAACCACTCAGGCTGATGTCATAGGACTGGCAGTAATAATCAGACTCA12640.11017314791006594No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12510.10904003800276305No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12460.10860422649995424No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG12450.10851706419939247No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12450.10851706419939247No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG12400.10808125269658367No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG12390.10799409039602191No Hit
ACATCAGCCTGAGTGGTTCGCGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC12350.10764544119377485No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12070.10520489677804556No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12060.10511773447748379No Hit
GTTTGGGGGCTGTTCCTGGAAGTTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAG12040.10494340987636026No Hit
GTACTTACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAGGGGACTGGAGTGGATTGGGAGTTTCTATTACAG12020.10476908527523676No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11980.1044204360729897No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11870.10346165076681034No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGG11830.1031130015645633No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG11820.10302583926400154No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11780.10267719006175448No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG11700.1019798916572604No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11600.10110826865164278No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11580.10093394405051925No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11570.10084678174995751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)