FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147285
Sequences flagged as poor quality0
Sequence length19-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATACTCCCCCTCTCTCGCACAGTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCATCTTCAGGGA50780.4426101622526225No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCATCTTCAGGGAGAATTGGTTCTTGGACGTGTCTCCT41470.361462060429623No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT38040.33156539133693896No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31720.27647881738190594No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCGCGAACCACTCAGGCTGATGTCATAG31550.27499705827235604No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31470.2742997598678619No Hit
GTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAGGGGACTGGAGTGGATT30440.26532204291000056No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAGCATCTCCT28990.2526835093285452No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGC27190.23699429522742824No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25350.2209564319240642No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG25020.2180800760055261No Hit
GTATAGTACCAGTCGATTCTATTACCACTATTACATGGACGTCTGGGGCA24530.21380912327799978No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT21570.18800908231171853No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21250.18521988869374217No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCATCATCTTTGGTAACACCAATCGGCCCTCA21210.18487123949149514No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21050.18347664268250696No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC21000.18304083117969816No Hit
GGTTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCATCATCTTTGGTAACACCAATCG20640.17990298835947477No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG19770.17231986821060155No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCGCGAACCACTC19610.17092527140161337No Hit
GTGTATTACTGTGCGAGAGAGGGGGAGTATAGTACCAGTCGATTCTATTA19190.1672644547780194No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAG19170.1670901301768959No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA18630.16238336594656078No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18580.16194755444375197No Hit
GATTATTACTGCCAGTCCTATGACATCAGCCTGAGTGGTTCGCGGGTATTCGGCGGAGGGACCAAGCTGACCGTC18070.15750227711510217No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18010.1569793033117316No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA17740.15462592119656407No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG17700.154277271994317No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC17510.15262118828364357No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCATCTTCAGGGAGAATTGGTTCTTGGACGTGTCTC17400.15166240297746417No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA17300.15079077997184656No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAGGG16920.14747861255049965No Hit
GTCCTATGACATCAGCCTGAGTGGTTCGCGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAA16720.14573536653926444No Hit
GACCATCACTGGGCTCCAGTCTGAAGATGAGTCTGATTATTACTGCCAGTCCTATGACATCAGCCTGAGTGGTTC16720.14573536653926444No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT16240.1415515761122999No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16180.14102860230892936No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15890.13850089559263828No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG15830.1379779217892677No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG15650.13640900037915601No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG15560.13562453967410015No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15250.13292250835668556No Hit
GTGATGGTCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC15070.13135358694657387No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC15040.13109210004488858No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA14930.1301333147387092No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCAGCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGAT14900.12987182783702395No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG14840.12934885403365337No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT14410.12560087510949763No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT14230.12403195369938595No Hit
GGGCAGAGGGTCAGCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCA14120.12307316839320656No Hit
TCACTGGGCTCCAGTCTGAAGATGAGTCTGATTATTACTGCCAGTCCTAT13870.12089411087916255No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13750.11984816327242141No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13740.11976100097185965No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG13710.11949951407017438No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG13620.11871505336511852No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13520.1178434303595009No Hit
GCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAACTTCCA13280.11575153514601864No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT12810.11165490701961586No Hit
GTATTACTGTGCGAGAGAGGGGGAGTATAGTACCAGTCGATTCTATTACC12810.11165490701961586No Hit
GTCTGAAGATGAGTCTGATTATTACTGCCAGTCCTATGACATCAGCCTGAGTGGTTCGCGGGTATTCGGCGGAGG12800.11156774471905412No Hit
GTCTGGCACCTCAGCCTCCCTGACCATCACTGGGCTCCAGTCTGAAGATG12670.11043463481175123No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGAT12630.11008598560950417No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12600.1098244987078189No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAGCATCTC12590.10973733640725714No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12590.10973733640725714No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT12500.10895287570220127No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC12380.10790692809546015No Hit
ACATCAGCCTGAGTGGTTCGCGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC12340.10755827889321311No Hit
CTATTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCACCATCCCCGACCAGCCCCAAGGTCTT12260.10686098048871902No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12200.10633800668534846No Hit
GTGCAGGAGATGCTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGGCTGCGTCAGCACAGACTGGGCCCAG11980.1044204360729897No Hit
GGCTGATGTCATAGGACTGGCAGTAATAATCAGACTCATCTTCAGACTGGAGCCCAGTGATGGTCAGGGAGGCT11910.10381029996905737No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG11790.10276435236231626No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11680.10180556705613687No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11670.10171840475557511No Hit
TCTTCAGACTGGAGCCCAGTGATGGTCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC11480.10006232104490168No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTA507.34494E-492.5197870
CCGCTAT507.34494E-492.5197870
CGCATAA550.00107259284.108970
AATGGTA3350.069.0446270
ACCCGGT700.002792566566.08555670
GTCTGTA850.006024424554.42340570
GTGGTAT2000.052.6371271
GCATTAG200.00783312650.939151
GGTAGAA25500.044.74813570
GGTATCA7400.043.596571
TGGTATC2450.042.9372442
TAAGTCA1805.194867E-542.83323770
GTATCAA19450.039.2846951
TGGGGCA16600.037.1565470
GCTTCTA850.004514551736.2822770
AGTGGAA15350.036.16408570
ACAGGGG1850.034.877261
CTCCATT1800.002680264434.26658670
AATGCTC400.002802354733.96684324
ATGACTT3201.1564553E-633.7311770
Produced by FastQC (version 0.11.5)