FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273239
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCGCGACAGTCAGAGAGGTCAGCTTCAGGGAGA57750.4535676334136796No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT48400.3801328737181315No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44740.35138728863944635No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGG38030.2986870493285236No Hit
GTCTCTGGTGGCTCCATCAGGAATGGTGCTTACTACTGGAGCTGGATCCGCCAGCACCCAGGGAAGGGCTTGGAG34310.2694702251501878No Hit
GTATGATAATCTCCCATTCACTTTCGGCCCTGGGACCAAAGTGGATGTCAGACGAACTGTGGCTGCACCA33440.26263725820525446No Hit
GTGTATTACTGTGCGGCAGCGACAGGCCCATTTCGGGATAGCACCAATTG33280.2613806206061863No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31650.24857862506567893No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT29910.23491269117581226No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27450.21559188809013863No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27200.21362839184159454No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26480.20797352264578764No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26180.20561732714753475No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25950.20381091059887424No Hit
ATATTACTGTCAACAGTATGATAATCTCCCATTCACTTTCGGCCCTGGGACCAAAGTGGATGTCAGACGAACTG24350.19124453460819218No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTTTTTAAATTGGTATCAGCAAAAA24310.19093037520842512No Hit
CAGTAATACACGGCCGTGTCCGCGACAGTCAGAGAGGTCAGCTTCAGGGAGAATTGGTTCTTAGACATGTCAA24090.18920249850970636No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC24060.18896687895988107No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23970.1882600203104052No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC23510.18464718721308412No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23310.18307639021424885No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCC22490.1766361225190243No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC22470.17647904281914079No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22440.17624342326931552No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA22050.17318036912158677No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT21030.16516930442752695No Hit
GTATTACTGTGCGGCAGCGACAGGCCCATTTCGGGATAGCACCAATTGGAGGAATTCTAGGTTCGACACCTGGGG20650.16218479012974No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT20250.15904319613206946No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC20160.1583363374825936No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19900.15629430138410777No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19410.1524458487369614No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGGAATGGTGCTTACTACTGGA17880.1404292516958717No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17580.13807305619761884No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACA17470.13720911784825943No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCAGTTCCA17320.13603102009913298No Hit
ACATATTACTGTCAACAGTATGATAATCTCCCATTCACTTTCGGCCCTGGGACCAAAGTGGATGTCAGACGAA17050.1339104441507054No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCAGTTCCA16700.13116154940274372No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16580.13021907120344256No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16310.12809849525501496No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16260.12770579600530615No Hit
GTGCAGCCACAGTTCGTCTGACATCCACTTTGGTCCCAGGGCCGAAAGTG15960.12534960050705327No Hit
AGTCAGGACATTAGCAACTTTTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTACGA15960.12534960050705327No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15850.1244856621576939No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG15800.12409296290798508No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15590.12244362605920804No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15310.1202445102608387No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15290.12008743056095518No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC15250.11977327116118812No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15170.11914495236165401No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15090.11851663356211992No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTTTTTAAATTGGTA14870.11678875686340115No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14410.11317592376608006No Hit
GTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAAAGTTGCTAATGTCCTGACTCGCCTGGCAAGTGA14390.11301884406619653No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGA14220.11168366661718657No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA14200.11152658691730302No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA14190.11144804706736126No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14160.11121242751753599No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTG13870.10893477186922486No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13810.1084635327695743No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG13500.10602879742137965No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13500.10602879742137965No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGACATCCACTTTGGTCC13340.10477215982231144No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATG13080.10273012372382562No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12890.10123786657493213No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG12860.10100224702510682No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12830.10076662747528155No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT12830.10076662747528155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA100.0026538342201.3879270
AACTGAA15400.078.4628370
TTGAACG556.335566E-573.2319870
TGTTAGA11800.049.49364570
CAACTGA13950.046.83667469
CGTATGC2300.046.25536741
ATGCCGT2550.044.50577544
CTAGCGC2450.044.43449828
ACTAGCG2450.044.4240127
TCGTATG2400.044.2929140
ATCTCGT2250.042.5571737
GTATCAA25450.041.9259641
GCGCTCA2650.041.13758531
TAGCGCT2650.041.1084429
GCCGTCT2800.039.4009446
CGAGACT2850.039.3546123
TATACAC2850.039.326793
GACTAGC2800.038.8664226
GTATGCC2850.038.57591242
CGCTCAT2750.038.4210332
Produced by FastQC (version 0.11.5)