FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169482
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT45880.3923104417169311No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41760.3570811692698135No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTGAGGGAGAACTGGTTCTTGGACGTGTCTACTGA41150.35186518475701206No Hit
GTATTACTGTCAACAACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTG40840.349214438529195No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31810.2720007661511678No Hit
GTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGCGAATTCTGTCCCAGATCTACTACCGCTGAATCTTGATGGGAC31310.2677253690095273No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT30710.2625948924395587No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30670.26225286066822745No Hit
CAGTAATACGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGCGAATTCTGTCCCAGATCTACTACCG29020.24814405010081386No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28910.24720346272965296No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG28770.24600635152999362No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGTAGTAGATCTGGGACAGAATTCG28080.24010630347452974No Hit
GTCTCCATCCTTCCTGTCTGCATCTATAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCAGGCCATTAGCAG27600.23600192221855487No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26030.22257717519380377No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25780.2204394766229835No Hit
CCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAACTGCTAATGGCCTGAC25260.21599306359567744No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25000.21376985708202434No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24540.2098364917117151No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC24140.20641617399840273No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23890.20427847542758248No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23730.2029103483422575No Hit
GTCTATTACTGTGCGAGAGTAAACGAGGAGGGGCTGCCCCGTTCCTACTA23680.20248280862809345No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC23160.19803639560078737No Hit
GTAGTAGATCTGGGACAGAATTCGCTCTCACAATCAGCAGCCTGCAGCCT22680.1939320143448125No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22010.18820298217501424No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT21320.18230293411955037No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20970.17931015612040202No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACA20840.1781985528635755No Hit
GTGGATGCACCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAACTGCTAA20820.17802753697790988No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20630.1764028860640865No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATG19770.16904920298046486No Hit
CACATGTACTGTCTCTGATGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA18830.16101145635418074No Hit
ACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA18430.15759113864086835No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18200.15562445595571373No Hit
GCCTGAAGACTTTGCAACGTATTACTGTCAACAACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGG18140.15511140829871686No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGTAGTAGATCTGGGACAGAATTCGCTCTCACAA18120.15494039241305124No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC18110.15485488447021845No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC17930.15331574149922786No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17760.1518621064710701No Hit
GTAATACGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGCGAATTCTGTCCCAGATCTACTACCG17450.149211360243253No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT17360.1484417887577577No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17070.14596205841560622No Hit
ACGTATTACTGTCAACAACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA16810.14373885190195318No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16550.1415156453883001No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16030.137069232360994No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16020.1369837244181612No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA15940.13629966087549872No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15640.13373442259051443No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15410.1317677399053598No Hit
GGCCATTAGCAGTTCTTTAGCCTGGTATCAGCAAACACCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATC15380.13151121607686136No Hit
GTCTCTGATGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGAT15370.13142570813402857No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15350.13125469224836295No Hit
CACCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATCCACTTTGCAA15350.13125469224836295No Hit
GGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAACTGCTAATGGCCTGACTGGCCCGGCAAGTGA15180.12980105722020518No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15120.12928800956320832No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14700.12569667596423031No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14510.12407202505040693No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14490.12390100916474132No Hit
ATCTACTACCGCTGAATCTTGATGGGACCCCACTTTGCAAAGTGGATGCACCATAGATCAGGAGGTTAGGGGCTT14100.12056619939426172No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14050.1201386596800977No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13990.11962561202310082No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13810.11808646905211026No Hit
GCTAATGGCCTGACTGGCCCGGCAAGTGATGGTGAT13650.1167183419667853No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13580.11611978636695564No Hit
ATCTTGATGGGACCCCACTTTGCAAAGTGGATGCACCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTGTT13560.11594877048129001No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13280.11355454808197134No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13180.11269946865364323No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13140.112357436882312No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA13120.11218642099664637No Hit
GAATTCGCTCTCACAATCAGCAGCCTGCAGCCTGAAGACTTTGCAACGTATTACTGTCAACAACTTAATATTTTC13070.11175888128248233No Hit
GTGGGGTCCCATCAAGATTCAGCGGTAGTAGATCTGGGACAGAATTCGCT13060.11167337333964952No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13050.1115878653968167No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG12660.10825305562633712No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG12640.10808203974067151No Hit
CAGTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTGAGGGAGAACTGGTTCTTGGACGTGTCTA12560.10739797619800905No Hit
CCATCCTTCCTGTCTGCATCTATAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCAGGCCATTAGCAGTTCT12480.10671391265534655No Hit
ATATTAAGTTGTTGACAGTAATACGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGCGAATTCTGTC12440.10637188088401532No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT12430.10628637294118251No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12430.10628637294118251No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12370.10577332528418565No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12210.1044051981988607No Hit
CATCAAGATTCAGCGGTAGTAGATCTGGGACAGAATTCGCTCTCACAATCAGCAGCCTGCAGCCTGAAGACTT12120.10363562671336539No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12110.10355011877053259No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12050.10303707111353573No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12050.10303707111353573No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11970.10235300757087326No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11970.10235300757087326No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11960.10226749962804044No Hit
GGACTGGAGTGGATTGGCCAAATCTTTTACAATGGGAATACCTACTACAA11880.10158343608537798No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11820.1010703884283811No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG11800.10089937254271548No Hit
CCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG11780.10072835665704988No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT11770.10064284871421705No Hit
TCTTTAGCCTGGTATCAGCAAACACCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATCCACTTTGCAAAG11720.10021530900005303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA500.003796993561.16102270
AGTACCG605.819693E-754.44480569
TAGTACC452.8732553E-547.36852368
TCGGAAT306.2537455E-446.0858147
TGTTAGA13650.044.80660670
GACGTAG2050.044.7270126
ACGAGAC2000.044.0621622
ACGTAGA2100.043.67144827
AGACGTA2100.043.63026425
CGTATGC1750.043.18084341
TCGTATG1600.042.8945540
CGATAGA405.2384356E-542.78882637
CACGAGA2200.041.5971421
CGTAGAG2150.041.08656728
GAGACGT2300.039.8073224
TATACAC2350.038.8903853
CTTATAC2450.038.6961981
CGAGACG2300.038.32312823
ATAGGCG451.0736078E-437.883832
GGCAAAG14550.037.83156270
Produced by FastQC (version 0.11.5)