FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169482
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45520.3892321557749499No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA40780.3487013908721981No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTGAGGGAGAACTGGTTCTTGGACGTGTCTACT40550.3467347081870435No Hit
GTATTACTGTCAACAACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGT39580.33844043773226096No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32410.2771312427211364No Hit
GTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGCGAATTCTGTCCCAGATCTACTACCGCTGAATCTTGATGGGAC30290.25900355884058074No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT29010.24805854215798107No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27950.23899470021770322No Hit
CAGTAATACGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGCGAATTCTGTCCCAGATCTACTACC27700.23685700164688298No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC27090.23164101713408158No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC26890.2299308582774254No Hit
GTCTCCATCCTTCCTGTCTGCATCTATAGGAGACAGAATCACCATCACTT26410.22582647702145053No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGTAGTAGATCTGGGACAGAATTC26110.22326123873646622No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24770.21180317439686971No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA24430.2088959043405542No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24310.2078698090265605No Hit
GTCTATTACTGTGCGAGAGTAAACGAGGAGGGGCTGCCCCGTTCCTACTACTACTACATGGACTTCTGGGGCAAA23820.20367991982775277No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23750.20308136422792314No Hit
CCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAACTGCTAATGGCCTGACTG22940.19615522085846554No Hit
GTAGTAGATCTGGGACAGAATTCGCTCTCACAATCAGCAGCCTGCAGCCT22360.1911957601741626No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21870.1870058709753549No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC21660.1852102041758659No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA21560.1843551247475378No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21410.18307250560504565No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA21390.18290148971938003No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20770.17759999726374584No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT20530.1755478066357584No Hit
GTGGATGCACCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAACTGCTAATG20200.17272604452227566No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20090.17178545715111476No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGT19940.17050283800862262No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19730.16870717120913362No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA19400.1658854090956509No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19070.1630636469821682No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT18990.16237958343950568No Hit
CACATGTACTGTCTCTGATGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGG18890.1615245040111776No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC18300.15647953538404183No Hit
GTAATACGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGCGAATTCTGTCCCAGATCTACTACC18280.1563085194983762No Hit
GCCTGAAGACTTTGCAACGTATTACTGTCAACAACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGGT18120.15494039241305124No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGTAGTAGA17660.151007027042742No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC17330.1481852649292593No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA16780.14348232807345473No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16150.13809532767498772No Hit
ACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT16090.13758228001799086No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15830.13535907350433782No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15830.13535907350433782No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTT15370.13142570813402857No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15290.13074164459136609No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15280.1306561366485333No Hit
GTCTCTGATGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATT15190.129886565163038No Hit
ACGTATTACTGTCAACAACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA15080.12894597779187708No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG14860.12706480304955528No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC14760.12620972362122718No Hit
ATCTACTACCGCTGAATCTTGATGGGACCCCACTTTGCAAAGTGGATGCA14760.12620972362122718No Hit
GGCCATTAGCAGTTCTTTAGCCTGGTATCAGCAAACACCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATC14580.1246705806502366No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT14500.12398651710757412No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14220.12159229470825544No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14170.12116475499409139No Hit
GTGGGGTCCCATCAAGATTCAGCGGTAGTAGATCTGGGACAGAATTCGCTCTCACAATCAGCAGCCTGCAGCCT14060.12022416762293049No Hit
CACCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCG13980.11954010408026802No Hit
GGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAACTGCTAATGGCCTGACTGGCCCGGCAAGT13720.11731689756661497No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA13660.1168038499096181No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG13550.11586326253845719No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13490.11535021488146034No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC13490.11535021488146034No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13170.11261396071081042No Hit
GCTAATGGCCTGACTGGCCCGGCAAGTGATGGTGATT13010.11124583362548547No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12940.1106472780256558No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12920.11047626213999018No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12920.11047626213999018No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA12850.10987770654016052No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG12790.10936465888316367No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12700.10859508739766838No Hit
CCATCCTTCCTGTCTGCATCTATAGGAGACAGAATCACCATCACTTGCCG12500.10688492854101217No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12470.10662840471251374No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12440.10637188088401532No Hit
ATATTAAGTTGTTGACAGTAATACGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGCGAATTCTGTC12390.10594434116985127No Hit
ATCTTGATGGGACCCCACTTTGCAAAGTGGATGCACCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTGTTT12370.10577332528418565No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA12180.10414867437036227No Hit
TCTTTAGCCTGGTATCAGCAAACACCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATCCACTTTGCAAAGT12020.10278054728503731No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11980.10243851551370606No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11970.10235300757087326No Hit
GAATTCGCTCTCACAATCAGCAGCCTGCAGCCTGAAGACTTTGCAACGTATTACTGTCAACAACTTAATATTTTC11860.10141242019971236No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11830.10115589637121393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAA403.1776977E-4114.2197170
AATGGTA2250.081.2229170
GTTATTA909.3510986E-567.6857570
AATCGAA1205.0764775E-663.45539570
CAAGTGT1001.5749958E-460.9171870
TAACGTA200.007749110551.07833532
CATACCG200.007749110551.07833532
GAGCAAA24200.043.42236770
GTATCAA18300.042.682211
CGGGACA1459.850458E-442.01184570
GACAGGT2600.039.0152051
GTGGTAT2000.038.885161
AAGGACA24700.038.2273870
ACTCAGT10950.036.1608870
GAGGGTT1750.002481429634.80981470
ATCAACG22600.034.07743
TCAACGC22800.033.926634
TATCAAC22850.033.8567352
GTTGACA17750.033.46154870
CAACGCA23200.033.3416865
Produced by FastQC (version 0.11.5)