FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483831
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48740.3284740647688315No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT43480.2930252838766679No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAATTTAAAT42970.2895882347787585No Hit
GTCCTGATCCGTGCTGCTTCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGACTCTGGGACAGAT37840.25501556444096396No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35960.242345657962396No Hit
GAGTTACAGTCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA34270.23095622075559818No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT31790.21424272710301914No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30960.20864909817897048No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30030.20238153805925335No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG29680.20002277887441358No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27930.18822898295021467No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27400.1846571476131716No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27390.18458975449360474No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA26970.181759243471797No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT26810.18068095355872738No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26680.1798048430043583No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26530.17879394621085556No Hit
GTCTACATACATTACAGTGGGAGCACCGGCCAGAATGCCTCCCTCAAGGGTCGAGTCTCCGCATCAGTAGACACG25470.17165027553676934No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC24470.16491096358008425No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23910.1611369488843406No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT23440.1579694722646986No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23300.1570259685907627No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC23210.15641943051466103No Hit
GTCTCCATCCTCCCTGTCTGCATCTGAAGGAGACACAGTCACCATCACTTGCCGGACAAGTCAGAACATTAACAG22880.15419545756895495No Hit
CAATAATATACGGCCGTGTCCGCGGCGGTCACAGACTTCAAGTTCAGGGAGAACTGGTTCCTGGACGTGTCTA22290.15021926351451076No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22230.14981490479710963No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTAAAGATGGTGAGAGTGAAATCTGTC22070.14873661488404002No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22030.14846704240577263No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21960.14799529056880467No Hit
ATAATATACGGCCGTGTCCGCGGCGGTCACAGACTTCAAGTTCAGGGAGAACTGGTTCCTGGACGTGTCTACTGA21650.1459061038622323No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA21280.14341255843825879No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21140.1424690547643229No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG20780.14004290245991627No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20660.13923418502511403No Hit
GGCCAAAAGTGTACGGAAGACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTAAAGA20580.13869504006857925No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC20570.1386276469490124No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20090.13539277720980356No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19820.13357316298149857No Hit
CTCTGGGACAGATTTCACTCTCACCATCTTTAGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAG19640.13236008682929526No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19590.13202312123146098No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19290.13000132764445546No Hit
GATTTCACTCTCACCATCTTTAGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTCT19070.12851867901398475No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACTCAGTC19050.12838389277485104No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAATTTAAATAGCTGTTAATGTTCTGACTTGTCCGGCAAGTGA18800.12669906478567977No Hit
CACTTGCACCGTCTCTGGTGGCTCCGTCAATAGTAATTACTATTGGAGCT18560.12508162991607535No Hit
TCTTTAGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGT18440.12427291248127315No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18150.12231851201383447No Hit
ACTTACTACTGTCAACAGAGTTACAGTCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA18090.12191415329643335No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17860.12036411154639579No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC17260.11632052437238474No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAAGA17190.11584877253541677No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTAAAGATGGTGAGAGTGAAATCTGTCCCAGAGTCACTGCCA17110.11530962757888197No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG17060.1149726619810477No Hit
GTCCCAGAGTCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATT17000.1145683032636466No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG16960.1142987307853792No Hit
ATATTATTGTGTGAGAGGAGGGAGGTATAGTGACTCTTTTGATATGTGGGGCCAAGGGACACTGGTCACCGTC16940.1141639445462455No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16860.1136247995897107No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG16670.11234433031794053No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16650.11220954407880682No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACA16640.11214215095923996No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT16560.11160300600270515No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16480.11106386104617035No Hit
GAGTGAAATCTGTCCCAGAGTCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGGAAGCAGCACGGA16050.10816595690479576No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15630.10533544588298802No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15610.10520065964385432No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15590.10506587340472062No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15220.10257232798074713No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG15210.10250493486118027No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15090.10169621742637806No Hit
GGACTGGAGTGGATTGTCTACATACATTACAGTGGGAGCACCGGCCAGAA15060.10149403806767753No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14980.10095489311114272No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14980.10095489311114272No Hit
CTATACCTCCCTCCTCTCACACAATAATATACGGCCGTGTCCGCGGCGGTCACAGACTTCAAGTTCAGGGAGAA14910.10048314127417475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA609.452083E-567.55131570
TGTCCGG16900.059.35721670
ATCGTAT252.640029E-454.88921441
AATGGTA3900.049.3644270
CTCTTAT1400.041.157081
CTTATAC3900.040.84671
TCGTATG3700.040.75015340
TGTTAGA13200.040.68431570
TATCTCG3550.040.3845536
CGTATGC3800.039.72245841
CGAGACG3850.039.629823
TATCGTA350.001393559439.16248740
CCTATCT3750.039.06933634
AGGCTCG1050.001495148638.6007570
GTGGTAT3350.038.4470021
GTATGCC4050.038.16829742
GCCGTCT3900.038.0384446
ATGCCGT4050.037.38653644
CGGACTC4000.037.35651428
ACGAGAC4100.037.20707722
Produced by FastQC (version 0.11.5)