FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483831
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA46630.31425411654022595No Hit
GTCCTGATCCGTGCTGCTTCCAATTTGCAAAGTGGGGTCCCATCAAGGTT42470.28621857880041596No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41930.282579350343806No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAATTTAAATAGCTGTTAATGTTCTGACTTGTCCG38960.26256359383245126No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37910.2554873162779319No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34170.23028228955992966No Hit
GAGTTACAGTCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT32690.2203081078640357No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC32510.21909503171183242No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT31920.2151188376573882No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG31490.21222093351601362No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA27710.18674633431974397No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27090.1825679609065992No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT25920.17468296591727764No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA25320.17063937874326657No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24910.1678762608410257No Hit
GTCTCCATCCTCCCTGTCTGCATCTGAAGGAGACACAGTCACCATCACTTGCCGGACAAGTCAGAACATTAACA24090.16235002503654392No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC23780.16026083832997154No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23730.1599238727321373No Hit
GTCTACATACATTACAGTGGGAGCACCGGCCAGAATGCCTCCCTCAAGGG23250.15668900299292843No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23220.1564868236342279No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22720.15311716765588534No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC22700.15298238141675163No Hit
CAATAATATACGGCCGTGTCCGCGGCGGTCACAGACTTCAAGTTCAGGGAGAACTGGTTCCTGGACGTGTCTA22280.1501518703949439No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC22200.1496127254384091No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21630.14577131762309858No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTAAAGATGGTGAGAGTGAAATCTGTC21460.14462563459046213No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA21410.14428866899262788No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG21140.1424690547643229No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC21040.14179512356865437No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG20800.14017768869904998No Hit
ATAATATACGGCCGTGTCCGCGGCGGTCACAGACTTCAAGTTCAGGGAGAACTGGTTCCTGGACGTGTCTACT20430.13768414327507647No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20340.13707760519897483No Hit
CTCTGGGACAGATTTCACTCTCACCATCTTTAGTCTGCAACCTGAGGATT20200.1361341015250389No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19700.13276444554669636No Hit
CACTTGCACCGTCTCTGGTGGCTCCGTCAATAGTAATTACTATTGGAGCTGGGTCCGGCAGTCCCCCGGGAAGG19560.13182094187276044No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19480.13128179691622563No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19240.1296643620466212No Hit
GGCCAAAAGTGTACGGAAGACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTAAA19220.1295295758074875No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGT18740.12629470606827867No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG18530.12487945055737479No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAATTTAAATAGCTGTTAATGTTCTGACTTGTCCGGCAAGT18290.12326201568777037No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT18080.1218467601768665No Hit
GATTTCACTCTCACCATCTTTAGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTCTT17710.11935321475289302No Hit
TCTTTAGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGT17500.11793795924198915No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACTCAGTCTCCATCCTCCCTGTCTGCATCTGAA17470.1177357798832886No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17390.11719663492675378No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17280.11645531061151844No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17080.1151074482201814No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA17020.1147030895027803No Hit
ACTTACTACTGTCAACAGAGTTACAGTCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA17010.11463569638321346No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16840.11349001335057698No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC16820.11335522711144329No Hit
GTCCCAGAGTCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGGAAGCAGCACGGATCAGGACCTTA16790.11315304775274272No Hit
GAGTGAAATCTGTCCCAGAGTCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGGAAGCAGCACG16610.11193997160053942No Hit
ATATTATTGTGTGAGAGGAGGGAGGTATAGTGACTCTTTTGATATGTGGGGCCAAGGGACACTGGTCACCGTCT16500.11119864728530406No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16240.10944642617656594No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAA16080.1083681362634963No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15810.10654852203519136No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT15770.10627894955692392No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15570.10493108716558691No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15540.10472890780688637No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15530.10466151468731952No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15450.1041223697307847No Hit
GTATGTAGACAATCCACTCCAGTCCCTTCCCGGGGGACTGCCGGACCCAGCTCCAATAGTAATTACTATTGACG15420.10392019037208415No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTAAAGATGGTGAGAGTGAAATCTGTCCCAGAGTCACTGCCA15360.10351583165468305No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGACTCTGGGACAGATTTCACTCTCACCATCTTTAGTCTGCAACCT15320.10324625917641564No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15180.10230275550247973No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC15010.10115707246984326No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT14930.10061792751330846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)