FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629887
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG26280.4172176914271925No Hit
GCCTTGGGCTGACCAAGGACGGTCAGAATGGTCCCCCCGCCGAACACCCCAACATTCAGGCTATTATCCCATG17870.28370167982511146No Hit
GGATAATAGCCTGAATGTTGGGGTGTTCGGCGGGGGGACCATTCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCC15810.2509974011211535No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15160.24067808987961334No Hit
CTCCTGCACAGCCTCTGGATTCAACTTCAAGAACTATGCTCTACACTGGGTCCGCCAGGCTCCAGGCAAGGGCC14690.23321643405880738No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG14460.229564985465647No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT12470.19797201720308566No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12410.1970194653961742No Hit
CTATTATCCCATGTGCCGCAGAAATAAGCGGCCTCGTCCCCAGTCTGGAG11950.18971656820985353No Hit
GCACTGGAGGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA11710.18590636098220792No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11700.1857476023477227No Hit
GCTCTACACTGGGTCCGCCAGGCTCCAGGCAAGGGCCTCGAGTGGGTGGC11220.17812718789243148No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT11190.17765091198897578No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11000.17463449793375638No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10840.17209435978199264No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGAATGGTCCCCC10820.17177684251302217No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10690.16971298026471413No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10630.16876042845780276No Hit
GTGTTCCCTGGCCCCAGTAGCCAGGATAACAGGAAAGAGTACAATATGAC10400.16510897986464237No Hit
CCCCAACATTCAGGCTATTATCCCATGTGCCGCAGAAATAAGCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC9920.15748856540935122No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC9910.15732980677486597No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9650.1532020822782499No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9580.15209077183685327No Hit
GTGTAGAGCATAGTTCTTGAAGTTGAATCCAGAGGCTGTGCAGGAGAGTC9550.1516144959333976No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA9530.1512969786644271No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9520.15113822002994187No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9420.14955063368508956No Hit
GTGCAGGAGAGTCTCAGGGACCTCCCAGGCTGGACCACGCCTCCCCCAGACTCAACCAGTTGCACCTGGCAC9350.1484393232436929No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9230.14653421962987012No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG9160.14542290918847348No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9140.14510539191950303No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG9080.1441528401125916No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCACCTCCAACATTGGGAATAATTATGTCTCCTGGTACCAACAA8970.14240649513325407No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8970.14240649513325407No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8510.13510359794693333No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8320.1320871838917139No Hit
GACCAAGGACGGTCAGAATGGTCCCCCCGCCGAACACCCCAACATTCAGG8210.13034083891237636No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8130.1290707698364945No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG8120.12891201120200926No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8090.12843573529855354No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8050.12780070076061262No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG7930.12589559714678983No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC7830.12430801080193749No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7810.12399049353296702No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7750.12303794172605562No Hit
ATGTTGGGGTGTTCGGCGGGGGGACCATTCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGT7750.12303794172605562No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCA7570.12018028630532143No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7520.11938649313289527No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG7520.11938649313289527No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC7450.11827518269149863No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7450.11827518269149863No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7450.11827518269149863No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAGACGGTGACCTGTGTTCCCTGG7390.11732263088458723No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG7390.11732263088458723No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7350.1166875963466463No Hit
GCCTCAGACATGACGACTCGGCTTTTTATTTCTGTGCGAGAGATCTGTCC7260.1152587686362792No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC7190.11414745819488256No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTGGAGGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACA7120.11303614775348594No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC7060.11208359594657455No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6980.11081352687069268No Hit
GCCCAGCACTGGAGGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAAC6950.11033725096723698No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG6880.10922594052584036No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGCTTATTTCTGCGGCA6860.10890842325686988No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6790.10779711281547326No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGCCAGGTGCAACTGGTTGAG6790.10779711281547326No Hit
ACACTGGGTCCGCCAGGCTCCAGGCAAGGGCCTCGAGTGGGTGGCAGCGATATCGTCTGATGGACATCAGAGGT6740.10700331964304709No Hit
CCCTGAGACTCTCCTGCACAGCCTCTGGATTCAACTTCAAGAACTATGCT6700.10636828510510615No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC6650.10557449193268No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGG6650.10557449193268No Hit
GTTTGGGGGCTGCTCCTGGGAATTGTTGGTACCAGGAGACATAATTATTC6600.10478069876025382No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG6520.10351062968437195No Hit
CTCGAGGCCCTTGCCTGGAGCCTGGCGGACCCAGTGTAGAGCATAGTTCT6450.10239931924297532No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT6450.10239931924297532No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT6430.10208180197400485No Hit
GTCTGAGGCTGTTCATTTCCAGGTACAGTGTGTTCTTGGAATTGTCTCTG6430.10208180197400485No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTGGAGG6360.10097049153260822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGAT252.7229852E-454.5416223
AGATACC200.007451976651.58320644
CCGAGTT200.007712504451.13682613
CCGTTTG200.007712504451.13682618
CACATAA250.00822999750.30218568
GTGGTAT1450.047.0335731
TGGTATC1450.047.0186422
GGTATCA5750.046.849531
AGGGGGG1551.04528226E-745.82430670
CAGGTAT606.213668E-945.4657861
AATGGTA1257.1332455E-540.5872470
CACGAGA1200.039.77308721
TCTCCGA1250.038.17913411
ATCTCGT901.3460522E-1038.01494637
AGGTATC451.0743297E-437.876132
CGTATGC1100.037.4341241
GTATGCC1151.8189894E-1235.82945342
GTATCAA12950.035.810891
TCGTATG1250.035.6558540
CGAGGCT1250.035.4830429
Produced by FastQC (version 0.11.5)