FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629887
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT24970.396420310309627No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16670.2646506436868835No Hit
GCCTTGGGCTGACCAAGGACGGTCAGAATGGTCCCCCCGCCGAACACCCCAACATTCAGGCTATTATCCCATGT14860.23591533084505634No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14780.23464526176917447No Hit
GGATAATAGCCTGAATGTTGGGGTGTTCGGCGGGGGGACCATTCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCC14680.23305767542432212No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14380.22829491638976512No Hit
CTCCTGCACAGCCTCTGGATTCAACTTCAAGAACTATGCTCTACACTGGGTCCGCCAGGCTCCAGGCAAGGGCCT12380.19654318949271854No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCT11840.18797022323051596No Hit
GCTCTACACTGGGTCCGCCAGGCTCCAGGCAAGGGCCTCGAGTGGGTGGCAGCGATATCGTCTGATGGACATCA10990.17447573929927115No Hit
GCACTGGAGGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGA10800.17145932524405172No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG10710.17003049753368463No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10680.16955422163022893No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC10540.16733160074743564No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10280.16320387625081959No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10270.16304511761633436No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10120.16066373809905587No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10100.1603462208300854No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA10060.15971118629214445No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG10040.159393669023174No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC9910.15732980677486597No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9520.15113822002994187No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC9080.1441528401125916No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9020.14320028830568024No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8940.14193021922979837No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCACCTCCAACATTGGGAA8830.1401838742504608No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA8730.13859628790560846No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG8620.1368499429262709No Hit
CTATTATCCCATGTGCCGCAGAAATAAGCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGGCTGAC8590.1363736670228152No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGAATGGTCCCCC8410.13351601160208101No Hit
GTGCAGGAGAGTCTCAGGGACCTCCCAGGCTGGACCACGCCTCCCCCAGACTCAACCAGTTGCACCTGGCACTG8390.13319849433311054No Hit
GTGTAGAGCATAGTTCTTGAAGTTGAATCCAGAGGCTGTGCAGGAGAGTCTCAGGGACCTCCCAGGCTGGACCAC8380.13303973569862532No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8220.13049959754686158No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA8200.13018208027789113No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG8000.12700690758818645No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAGACGGTGACCTGTGTTCCCTGGCCCCAGTAGCCAGGATAACAGGAAA7990.12684814895370122No Hit
ATGTTGGGGTGTTCGGCGGGGGGACCATTCTGACCGTCCTTGGTCAGCCC7980.12668939031921597No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7950.12621311441576027No Hit
GTCTGAGGCTGTTCATTTCCAGGTACAGTGTGTTCTTGGAATTGTCTCTGGAGATGGTGAATCGGCCCTTCACG7940.12605435578127505No Hit
CCCCAACATTCAGGCTATTATCCCATGTGCCGCAGAAATAAGCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC7860.1247842867053932No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7810.12399049353296702No Hit
GTGTTCCCTGGCCCCAGTAGCCAGGATAACAGGAAAGAGTACAATATGAC7810.12399049353296702No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7800.12383173489848179No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC7660.12160911401568854No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCAT7610.12081532084326235No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT7250.11510001000179398No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG7180.11398869956039735No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7160.11367118229142688No Hit
GACCAAGGACGGTCAGAATGGTCCCCCCGCCGAACACCCCAACATTCAGG7080.11240111321554501No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6780.10763835418098802No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG6760.10732083691201756No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC6730.10684456100856184No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT6720.10668580237407663No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT6670.10589200920165046No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG6550.10398690558782765No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC6550.10398690558782765No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG6550.10398690558782765No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6540.10382814695334243No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG6530.10366938831885719No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6510.10335187104988672No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG6510.10335187104988672No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT6420.10192304333951964No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA6420.10192304333951964No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT6400.10160552607054915No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTGGAGG6380.1012880088015787No Hit
ACACTGGGTCCGCCAGGCTCCAGGCAAGGGCCTCGAGTGGGTGGCAGCGATATCGTCTGATGGACATCAGAGGTT6360.10097049153260822No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG6350.10081173289812301No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT6300.10001793972569684No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGCTTATTTCTGCGGCACATGGGATAATAGCCTGAATGTTGG6300.10001793972569684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT200.0061879084151.7126570
GTGATAA455.1494734E-4101.1417670
AATGGTA1205.8331352E-875.85631670
AAATTAT750.003911166560.6850570
ACCTCCA9600.055.31189770
CGCGCAA200.006961629352.47875261
ACCGCGC200.00778222651.0205428
GGTAGAA8650.049.10929570
CAGAGCA4850.046.92143670
GTTTAAC306.674245E-445.4779633
GGTATCA3650.044.776551
AGAAATA16850.040.5167370
GTCATAT5650.040.2776970
TGATACC350.00144472438.8727955
AAGCCCG350.00144472438.8727955
GTATCAA8850.038.8579941
GGCGTCA11950.038.08685370
AACAAAT2001.0614908E-437.92816570
AGTGGAA6400.037.9281670
TAACCGT400.002548152337.9281670
Produced by FastQC (version 0.11.5)