FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286672
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT56270.4373297934516333No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGACCTCAGCTTCAGGGAAAACTGGTTCTTGGACGTGTCTACTGA53560.4162677045898256No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52090.404842881480284No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG38130.2963459218821891No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36610.28453249934715297No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT36450.2832889811855702No Hit
GAGTTATCGTCTCCCGTGGACGTTCGGCCCAGGGGCCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCA34130.26525796784262035No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33100.2572528196774314No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32350.25142382829501225No Hit
GTCTCTGGTGGCTCCATCGGTACTTACTACTGGACCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGAT31230.242719201163933No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAATCTACAACCTGAAGATTTTGCAACTTA29990.2330819354116667No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29850.23199385702028177No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29590.2299731400077098No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC29350.2281078627653357No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29100.22616486563786264No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG28820.2239887088550928No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28700.22305607023390575No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27680.21512864195381573No Hit
GTGTATTACTGTGCGAGAGTCTCGGAGGCAAGTACCAGTCTCTACTACTA26440.20549137620154945No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT26330.20463645746546127No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26180.20347065918897744No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGAGCATTGGCACCGATTTAAATTGGTT25900.20129450240620764No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC25830.20075046321051518No Hit
CCATAGATTAGGAGCCTAGGGGCTTTCCCTGGTGTCTGCTGAAACCAATTTAAATCGGTGCCAATGCTCTGGCT24620.19134635711354564No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG23860.18543964584602757No Hit
AATCTATGGTGCTTCCAGTTTACAAAGTGGGGTCCCCACAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA23850.18536192596092868No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTTTCCATCCTCCCTGTCTGCATCTG23780.1848178867652362No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGCCCCTGGGCCGAACGTC22280.1731599040003979No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGCCC22250.17292674434510116No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22130.1719941057239141No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACA21520.16725319273287986No Hit
GATTTCACTCTCACCATCAGCAATCTACAACCTGAAGATTTTGCAACTTACCACTGTCACCAGAGTTATCGTCTC21370.16608739445639606No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC20900.1624345598567467No Hit
CACCAGGGAAAGCCCCTAGGCTCCTAATCTATGGTGCTTCCAGTTTACAAAGTGGGGTCCCCACAAGGTTCAG20680.16072472238457045No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20360.15823768606140493No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGACCTCAGCTTCAGGGAAAACTGGTTCTTGGACGTGTCTA19860.15435169180645883No Hit
AGCCAGAGCATTGGCACCGATTTAAATTGGTTTCAGCAGACACCAGGGAAAGCCCCTAGGCTCCTAATCTATGG19720.15326361341507394No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGTGGGGACCCCACTTTGTAAACTGGAAGCACCATAGA19470.15132061628760088No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19290.14992165835582028No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19200.14922217938993No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19160.1489112998495343No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGAGCATTGGCACCGATTTAAATTGGTTTCAGCAGACA19080.14828954076874293No Hit
GTATTACTGTGCGAGAGTCTCGGAGGCAAGTACCAGTCTCTACTACTACT19040.14797866122834724No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18600.1445589862839947No Hit
CGATAACTCTGGTGACAGTGGTAAGTTGCAAAATCTTCAGGTTGTAGATTGCTGATGGTGAGAGTGAAATCTGTC18570.14432582662869792No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18520.1439372272032033No Hit
GTTTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGAGCATTGGCAC18360.14269370904162054No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTTTCCATCCTCC18300.14222738973102703No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17890.13904087444197122No Hit
GCCCAGGGGCCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTC17720.13771963639528956No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17670.13733103696979496No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17370.1349994404168273No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA17130.13313416317445317No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16550.1286264098387157No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16260.12637253317084696No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT16200.1259062138602534No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT16120.12528445477946207No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16060.12481813546886852No Hit
ACTTACCACTGTCACCAGAGTTATCGTCTCCCGTGGACGTTCGGCCCAGGGGCCAAGGTGGAAGTCAAACGAA15870.12334145765198902No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG15810.12287513834139548No Hit
GATTAGGAGCCTAGGGGCTTTCCCTGGTGTCTGCTGAAACCAATTTAAAT15800.12279741845629656No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15560.12093214121392241No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCGGTACTTACTACTGGACCTGGATCCGGCAGCCCCCAGGGAAGGGA15530.12069898155862566No Hit
CCCCTAGGCTCCTAATCTATGGTGCTTCCAGTTTACAAAGTGGGGTCCCCACAAGGTTCAGTGGCAGTGGATC15290.11883370431625155No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15280.11875598443115262No Hit
ACCTTGTGGGGACCCCACTTTGTAAACTGGAAGCACCATAGATTAGGAGCCTAGGGGCTTTCCCTGGTGTC15060.11704614695897633No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT15030.11681298730367957No Hit
GTCCACGGGAGACGATAACTCTGGTGACAGTGGTAAGTTGCAAAATCTTC15030.11681298730367957No Hit
CCCGTGGACGTTCGGCCCAGGGGCCAAGGTGGAAGTCAAACGAACTGTGG15020.11673526741858066No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG15000.1165798276483828No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14890.11572490891229466No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14860.1154917492569979No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14660.11393735155501947No Hit
GGATATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACG14470.11246067373813995No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14450.11230523396794209No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14330.11137259534675505No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14290.11106171580635936No Hit
ATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT13980.10865239936829277No Hit
GCCTAGGGGCTTTCCCTGGTGTCTGCTGAAACCAATTTAAATCGGTGCCAATGCTCTGGCTTGCCCGGCAAGTGA13930.10826379994279815No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13870.10779748063220462No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13710.10655396247062189No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13620.10585448350473159No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13530.10515500453884129No Hit
TCCCTGGTGTCTGCTGAAACCAATTTAAATCGGTGCCAATGCTCTGGCTTGCCCGGCAAGTGATGGTGAC13480.10476640511334669No Hit
GTGGTAAGTTGCAAAATCTTCAGGTTGTAGATTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA13450.1045332454580499No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13440.104455525572951No Hit
GTAATAGATATATCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGATCCAGGTCCAGTAGTAAGTACCGA13430.10437780568785207No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG13410.10422236591765423No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGA13380.10398920626235747No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT13370.10391148637725854No Hit
CCACAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAATCTACAACCTGAAGATTT13370.10391148637725854No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13360.10383376649215961No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13220.10274568810077472No Hit
CTCCTAATCTATGGTGCTTCCAGTTTACAAAGTGGGGTCCCCACAAGGTTCAGTGGCAGTGGATCTGGGACAGAT13160.10227936879018118No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13030.10126901028389519No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13030.10126901028389519No Hit
CTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCA13030.10126901028389519No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13020.10119129039879629No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12890.10018093189251029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTAGA13250.055.19185670
GTACGTA402.6843537E-454.7014369
GGTATCA9250.044.3511541
GTGGTAT2300.042.7495421
CTTATAC4050.042.6949421
TATACAC4150.042.474883
CGTATGC3250.042.20141641
TCGTATG3300.041.5260940
AAAGGGG3550.040.89546268
TCGACGT4100.040.62301629
TTATACA4350.039.742742
CGACGTC4350.039.08193630
ATGCCGT3800.038.9089144
CCCACGA4450.038.8617319
GACGTCA4300.038.76378631
TACACAT4500.038.4194765
ACGTCAT4000.038.29324332
CTCGACG4350.038.2659928
AGACTCG4350.038.21836525
ACGAGAC4450.038.11158422
Produced by FastQC (version 0.11.5)