FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286672
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54840.42621584988248756No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA51030.3966045736597983No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGACCTCAGCTTCAGGGAAAACTGGTTCTTGGACGTGTCTACT50610.3933403384856436No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45140.3508275613365333No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG37410.2907500901550667No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT35910.27909210739022844No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34550.2685222030167751No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC33640.2614496934727732No Hit
GAGTTATCGTCTCCCGTGGACGTTCGGCCCAGGGGCCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT32110.24955855105263813No Hit
GTCTCTGGTGGCTCCATCGGTACTTACTACTGGACCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATT30450.23665705012621707No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC29220.22709750425904968No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA28500.22150167253192732No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28350.2203358742554435No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGA27620.21466232264322221No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27610.21458460275812327No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAATCTACAACCTGAAGATTTTGCAACTTA27530.2139628436773319No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC27510.21380740390713407No Hit
GTGTATTACTGTGCGAGAGTCTCGGAGGCAAGTACCAGTCTCTACTACTACTATTACATGGACGTCTGGGGCAAA26950.20945509034159443No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG25850.20090590298071304No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25190.1957763905641842No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA25140.19538779113868956No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGT24630.19142407699864458No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24390.18955879975627044No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTTTCCATCCTCCCTGTCTGCATCTGTG23790.18489560665033514No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT23060.17922205503811384No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGAGCATTGGCACCGATTTAAATTGGTTTCAGCAGACA22590.17556922043846449No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22400.174092542621585No Hit
CCATAGATTAGGAGCCTAGGGGCTTTCCCTGGTGTCTGCTGAAACCAATTTAAATCGGTGCCAATGCTCTGGCTT22230.1727713045749033No Hit
AATCTATGGTGCTTCCAGTTTACAAAGTGGGGTCCCCACAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA22050.17137234664312273No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC21830.16966250917094644No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGCCCCTGGGCCGAACGTC21750.16904075009015507No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA21590.16779723192857232No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGCCCCTGGGCCGAACGTCCACGGGAGAC20880.16227912008654888No Hit
AGCCAGAGCATTGGCACCGATTTAAATTGGTTTCAGCAGACACCAGGGAAAGCCCCTAGGCTCCTAATCTATGGT19850.15427397192135991No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT19700.15310817364487608No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGACCTCAGCTTCAGGGAAAACTGGTTCTTGGACGTGTCTA19640.15264185433428254No Hit
GATTTCACTCTCACCATCAGCAATCTACAACCTGAAGATTTTGCAACTTACCACTGTCACCAGAGTTATCGTCTC19360.15046569755151276No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGTGGGGACCCCACTTTGTAAACTGGAAGCACCATA19250.14961077881542462No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19250.14961077881542462No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19130.14867814019423753No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA19020.14782322145814938No Hit
CACCAGGGAAAGCCCCTAGGCTCCTAATCTATGGTGCTTCCAGTTTACAAAGTGGGGTCCCCACAAGGTTCAGTG18800.14611338398597312No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17590.13670927788900356No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTTTCCATCCTCC17460.1356989193827176No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC17290.13437768133603592No Hit
GTATTACTGTGCGAGAGTCTCGGAGGCAAGTACCAGTCTCTACTACTACTATTACATGGACGTCTGGGGCAAAGG17280.134299961450937No Hit
CGATAACTCTGGTGACAGTGGTAAGTTGCAAAATCTTCAGGTTGTAGATTGCTGATGGTGAGAGTGAAATCTGTC17260.13414452168073915No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17120.13305644328935423No Hit
GTTTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGAGCATTGGCAC17110.13297872340425532No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG17100.1329010035191564No Hit
GCCCAGGGGCCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTC16980.13196836489796934No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16850.13095800639168334No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCA16740.1301030876555952No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGAT16700.12979220811519954No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16020.12450725592847284No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCGGTACTTACTACTGGACCTGGATCCGGCAGCCCCCAGGGAAGG16020.12450725592847284No Hit
CCCCTAGGCTCCTAATCTATGGTGCTTCCAGTTTACAAAGTGGGGTCCCCACAAGGTTCAGTGGCAGTGGATCTG15540.12077670144372459No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT15440.11999950259273537No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG15380.11953318328214184No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15250.11852282477585585No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15080.11720158672917419No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15050.11696842707387742No Hit
CTCCTAATCTATGGTGCTTCCAGTTTACAAAGTGGGGTCCCCACAAGGTT15040.11689070718877849No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA14910.11588034868249251No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14880.11564718902719573No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14840.11533630948680006No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14730.11448139075071191No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14670.11401507144011838No Hit
GTGGTAAGTTGCAAAATCTTCAGGTTGTAGATTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA14630.1137041918997227No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14310.11121715557655719No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14300.11113943569145826No Hit
ACTTACCACTGTCACCAGAGTTATCGTCTCCCGTGGACGTTCGGCCCAGGGGCCAAGGTGGAAGTCAAACGAA14230.11059539649576583No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14190.11028451695537013No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14120.10974047775967768No Hit
ACCTTGTGGGGACCCCACTTTGTAAACTGGAAGCACCATAGATTAGGAGCCTAGGGGCTTTCCCTGGTGTCT14100.10958503798947983No Hit
GGATATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAG14030.10904099879378737No Hit
ATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAA13880.10787520051730355No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13840.10756432097690787No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC13750.10686484201101758No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA13670.10624308293022619No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG13450.1045332454580499No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13400.1041446460325553No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13350.10375604660706068No Hit
GCATTGGCACCGATTTAAATTGGTTTCAGCAGACACCAGGGAAAGCCCCTAGGCTCCTAATCTATGGTGCTTCCA13190.10251252844547794No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13040.10134673016899412No Hit
GTCCACGGGAGACGATAACTCTGGTGACAGTGGTAAGTTGCAAAATCTTCAGGTTGTAGATTGCTGATGGTGAGA12950.10064725120310382No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12930.10049181143290598No Hit
GATTAGGAGCCTAGGGGCTTTCCCTGGTGTCTGCTGAAACCAATTTAAATCGGTGCCAATGCTCTGGCTTGCCCG12930.10049181143290598No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12920.10041409154780705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAT550.001021684685.1473270
TGCACTA650.001982888772.0477470
ATACAAA951.08149856E-465.7277570
GTGGTAT2500.056.8595621
TATGGTT850.005739571555.0953370
GGTGCAT850.005739571555.0953370
CGGCAAA1152.781454E-454.29684470
AACCTTT1152.781454E-454.29684470
TGGTATC2700.052.6006852
AATGGTA3650.051.32167470
CGAAGTA250.00857547249.78518368
TAATTTT1305.093677E-448.03182670
GAGCCAT11850.044.7891770
TTGGTTT26650.044.517370
GAGCAAA27550.044.1962570
GGTATCA9350.042.7134481
GATTCAC2100.041.9033051
GGACTCA1500.001030737841.62758370
ATTGACA9650.040.44130370
ATTCACT2200.039.9628562
Produced by FastQC (version 0.11.5)