FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162679
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC175461.5091009642386248No Hit
GTATAATAGTTACCCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA77830.6694023027852055No Hit
GAGTATTAGTGAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTCAGCTGCTGAGTTATAGGGCG75030.6453199894381854No Hit
CCCTATAACTCAGCAGCTGAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACTCACTAATACTCTGA47080.4049268972777525No Hit
GTTATAGGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCAC39930.34343098998089755No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGA39400.33887255209735445No Hit
GTATTAGTGAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT38820.3338840729040432No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37590.32330505668374504No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT36820.3166824205133145No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTGAGTGGTTGGCCTGGTA32880.28279516530357907No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC32480.27935483482543333No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26820.23067415855967124No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT26400.2270618115576182No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25990.22353547281751887No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25780.22172929931649235No Hit
GAGTTATAGGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA24970.21476263009824725No Hit
CACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACAGGGTGGAAGGAGA24370.20960213438102862No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC24240.2084840269756313No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTGAGTGG23140.1990231181607305No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22870.19670089508798216No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22430.19291653156202185No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATG21990.18913216803606153No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21990.18913216803606153No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21890.18827208541652513No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21190.1822515070797701No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACA20680.1778650857201343No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG20020.1721885404311938No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC19510.167802119071558No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19090.164189772069505No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC18940.16289964814020036No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT18350.15782516068493538No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18290.15730911111321355No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17040.14655807836900814No Hit
CTATAACTCAGCAGCTGAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACTCACTAATACTCTGAC16870.1450959379157962No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC16800.1444938800821207No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACTAGACGCCCTATAA16430.14131157438983588No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT16370.14079552481811403No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTAC16030.13787124391169014No Hit
GTAATACACGGCTGTGTCCTCGGTTTCCAGGCTGTTCATTTGCAGATATA15970.1373551943399683No Hit
CAGTATAATAGTTACCCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15900.1367531365062928No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTGAGTGGTTGGCCTGGTATCAGCAGAAA15550.13374284733791528No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14920.12832432683483574No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14680.1262601285479483No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCCTATAACTCAGCAGCTGAGGGGCTTTCCCTGGTTTC14440.12419593026106088No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14320.12316383111761715No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14310.1230778228556635No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14310.1230778228556635No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14200.12213173197417343No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCTTTAACGCCTGG13900.11955148411556414No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13850.11912144280579592No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT13730.1180893436623522No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13660.1174872858286767No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13310.11447699666029917No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTCAGCTGCTGAGTTATAGGGCGTCTAGTTTAGAAAGTGGGGTCCCA13270.11413296361248461No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC12970.11155271575387532No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12480.10733831091814679No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGA12390.106564236560564No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12270.10553213741712028No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12120.10424201348781564No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG12100.10406999696390837No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC12080.10389798044000105No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATTG12020.10338193086827921No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCTTTAACGCCTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG11900.10234983172483549No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11900.10234983172483549No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)