FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162679
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC174991.5050585759268036No Hit
GTATAATAGTTACCCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT73440.6316446757875561No Hit
GAGTATTAGTGAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTCAGCTGCTGAGTTATAGGGCGT65040.5593977357464958No Hit
CCCTATAACTCAGCAGCTGAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACTCACTAATACTCT45090.38781125314897746No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37210.3200367427295066No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACAGGGTGGAAGGAGACTGG36670.31539229658400986No Hit
GTTATAGGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT36660.3153062883220562No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA35260.3032651316485461No Hit
GTATTAGTGAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTCAGCTGCTGAGTTATAGGGCGTCTA35140.3022330325051025No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30680.26387334767377757No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29790.2562186123599033No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA29520.2538963892871549No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26690.22955605115427385No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT24550.21115028309619424No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC23980.20624781216483654No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC23900.2055597460692074No Hit
GAGTTATAGGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA23820.20487167997357827No Hit
CACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACAGGGTGGAAGGA23800.20469966344967097No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23010.19790501075533318No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGT22440.1930025398239755No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACTAGACGCCCTATAA22220.19111035806099536No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA21070.18121940793632635No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG20970.18035932531678991No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20140.17322063957463754No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19700.16943627604867723No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC19370.166598003404207No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18860.1622115820445712No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC18860.1622115820445712No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18340.15773915242298175No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT18070.15541692935023338No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC17400.14965437579933927No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA17250.14836425187003463No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTGAGTGGTTGGCCTGGTATCAGCAGAAA16670.14337577267672333No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16410.1411395578659286No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16290.14010745872248487No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA15800.13589305388675635No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15720.13520498779112722No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA15590.13408688038572986No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGAT15310.13167864905102783No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTAC15210.13081856643149142No Hit
CAGTATAATAGTTACCCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15060.12952844250218676No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14980.12884037640655763No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT14950.1285823516206967No Hit
CTATAACTCAGCAGCTGAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACTCACTAATACTCTGACTG14730.12669016985771653No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCCTATAACTCAGCAGCTGAGGGGCTTTCCCTGGTTTCT14120.1214436658785443No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCTTTAACGCCTGGATGAGTTGGGTCCGCCAGGCTCCAG13470.11585312885155749No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTGA13460.11576712058960384No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13420.11542308754178927No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12820.11026259182457068No Hit
GTAATACACGGCTGTGTCCTCGGTTTCCAGGCTGTTCATTTGCAGATATAGTGTGTTTTCTGAATCATCTCTTGA12650.10880045137135874No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCTTTAACGCCTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGG12550.1079403687518223No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12540.10785436048986867No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12430.10690826960837857No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12280.10561814567907393No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGA11890.10226382346288185No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC11870.10209180693897457No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA455.483655E-499.5792270
CCGCTAT1701.0286385E-861.50481470
CTTCTAA5700.047.16910670
CTTACCG306.9419364E-445.1196259
GTGGTAT3700.044.864931
TTCTGTC51550.042.8365569
ATTCTGT41100.041.86618868
GAGCAAA23650.041.68432670
GTATCAA23950.039.1820221
AATTCTG40950.038.30395567
CTATTAT39300.037.7567141
TATTATA40100.037.304452
AGAGTGA41550.036.80983761
GAATTCT41300.036.7130766
TGAGAGT41950.036.21161759
CGTCACA11650.035.89980370
TGAATTC41750.035.84083665
GGCAGTA41050.035.78772414
TATACTG41600.035.7106675
TTGGCAG41150.035.6992212
Produced by FastQC (version 0.11.5)