FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692813
Sequences flagged as poor quality0
Sequence length19-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGCCTGTCCTATGACAGCAGCCTCTATGGTTATGTCTTCGG26950.38899385548481336No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC26360.3804778490011013No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGCACGGTGACCTTGGTCCCAG25930.3742712680044976No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG25910.37398258981860905No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG23080.33313462651538006No Hit
GGTTATGATGTACACTGGTACCTGCAACTTCCAGGAACTGCCCCCAAACTCCTCATCTATGGTAATAACAATCGG22920.33082520102827173No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC22690.32750540189055344No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG20410.2945960886992594No Hit
GTCATAGGACAGGCAGTAATAATCAGCCTCATCGTCAGCCTGGAGTCCAG20200.2915649677474297No Hit
CTCCTGCACTGGGAGCAGTTCCAATATCGGGACAGGTTATGATGTACACTGGTACCTGCAACTTCCAGGAACTGC17020.24566513619115113No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGCACGGTGACCTTGGTC16990.24523211891231833No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT16920.24422174526170845No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16370.23628309514977347No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT16300.23527272149916353No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16180.2335406523838323No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGTCCAGTGATGGCCAGGGAGG16140.2329632960120552No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG16000.23094254871083542No Hit
GTCCTATGACAGCAGCCTCTATGGTTATGTCTTCGGCACTGGGACCAAGGTCACCGTGCTAGGTCAGCCCAAGGC15830.22848878413078277No Hit
GCCTTGGGCTGACCTAGCACGGTGACCTTGGTCCCAGTGCCGAAGACATA15300.22083881220473633No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14950.2157869439516868No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14040.20265208649375807No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG13970.20164171284314816No Hit
TCACTGGACTCCAGGCTGACGATGAGGCTGATTATTACTGCCTGTCCTATGACAGCAGCCTCTATGGTTATGTCT13910.2007756782854825No Hit
ACCATAGAGGCTGCTGTCATAGGACAGGCAGTAATAATCAGCCTCATCGT13190.19038326359349492No Hit
GACCTAGCACGGTGACCTTGGTCCCAGTGCCGAAGACATAACCATAGAGG12320.17782576250734325No Hit
GTGCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC12270.17710406704262188No Hit
GATGTACACTGGTACCTGCAACTTCCAGGAACTGCCCCCAAACTCCTCAT12070.17421728518373644No Hit
GTTCCAATATCGGGACAGGTTATGATGTACACTGGTACCTGCAACTTCCA11300.1631031750270275No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC11300.1631031750270275No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC11200.16165978409758477No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10950.15805130677397797No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10400.15011265666204301No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA10340.1492466221043774No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT10130.14621550115254767No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGTTCCAATATCGGGACAGGTTATGATGTACACTGGTACC9930.1433287192936622No Hit
CCATAGAGGCTGCTGTCATAGGACAGGCAGTAATAATCAGCCTCATCGTC9860.14231834564305232No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG9750.14073061562066533No Hit
GGCCATCACTGGACTCCAGGCTGACGATGAGGCTGATTATTACTGCCTGTCCTATGACAGCAGCCTCTATGGTTA9650.1392872246912226No Hit
GTCCAGTGATGGCCAGGGAGGCAGAGGTGCCAGACTTGGAACCAGAGAATCGGTCAGGGACCCCTGAGGGCCGAT9630.13899854650533405No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGTTCCAATATCGGGACAGGTTATGA9540.1376994946688356No Hit
ACCTAGCACGGTGACCTTGGTCCCAGTGCCGAAGACATAACCATAGAGGC9300.13423535643817308No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAG9300.13423535643817308No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9270.13380233915934026No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG9220.1330806436946189No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9210.13293630460167463No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG9010.1300495227427892No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8830.12745141906979227No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8820.127307079976848No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG8590.12398728083912974No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG8340.12037880351552295No Hit
GTGTACATCATAACCTGTCCCGATATTGGAACTGCTCCCAGTGCAGGAGA8240.11893541258608023No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8220.11864673440019169No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8130.11734768256369324No Hit
GTCTGGCACCTCTGCCTCCCTGGCCATCACTGGACTCCAGGCTGACGATGAGGCTGATTATTACTGCCTGTCCTA8130.11734768256369324No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8010.11561561344836198No Hit
CTCCTCATCTATGGTAATAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGTTCCAAGTCTGGCACC7940.11460523979775206No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAG7780.11229581431064371No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG7720.11142977975297808No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTT7710.11128544066003379No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGT7700.11114110156708952No Hit
CATCATAACCTGTCCCGATATTGGAACTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACA7700.11114110156708952No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA7690.11099676247414526No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG7680.11085242338120099No Hit
ATATTGGAACTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGC7570.109264693358814No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC7420.10709960696464992No Hit
TGCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG7370.10637791149992855No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7360.10623357240698429No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG7320.1056562160352072No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA7210.1040684860128202No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7160.10334679054809882No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG7150.10320245145515455No Hit
GTGATGGCCAGGGAGGCAGAGGTGCCAGACTTGGAACCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTGTTA7040.10161472143276756No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7030.1014703823398233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3600.0101.35606470
CATTAGA600.00714391152.12597770
GAACTAG650.00980218648.11628770
GGTATCA7650.046.274671
AATGGTA3400.045.9935170
AATCTCG3550.041.19708336
TCGTATG3950.040.72453740
TCTCGCG5650.040.59367870
TCTCGTA3650.040.18254538
CTTATAC4050.039.957811
CGTATGC3950.039.90192841
CTAATCT3750.039.81118834
AAAGGGG3900.039.61633368
GCTAATC3900.039.1073633
AAAAGGG3600.038.87640467
ATCTCGT3800.038.52041637
TATACAC4300.038.4048733
ATGCCGT3950.038.36694744
GTGGCCG3009.094947E-1238.2257270
CGAGACT4250.038.0719523
Produced by FastQC (version 0.11.5)