FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692813
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38510.5558498469283919No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC26200.378168423513993No Hit
GATTATTACTGCCTGTCCTATGACAGCAGCCTCTATGGTTATGTCTTCGGCACTGGGACCAAGGTCACCGTGCTA25360.3660439397066741No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT24890.3592600023382933No Hit
GGTTATGATGTACACTGGTACCTGCAACTTCCAGGAACTGCCCCCAAACTCCTCATCTATGGTAATAACAATCG22410.3234639072881138No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21630.31220545803846056No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC20990.3029677560900272No Hit
CTCCTGCACTGGGAGCAGTTCCAATATCGGGACAGGTTATGATGTACACTGGTACCTGCAACTTCCAGGAACTGC18100.26125375822913255No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG18060.2606764018573554No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT17480.2523047344665877No Hit
GTCCTGGGCCCAGTCTGTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCT16930.2443660843546527No Hit
GTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC16560.23902553791571463No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15710.2267567150154515No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT15160.21881806490351655No Hit
GTCCTATGACAGCAGCCTCTATGGTTATGTCTTCGGCACTGGGACCAAGGTCACCGTGCTAGGTCAGCCCAAGGC14890.2149209093940212No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT14750.21290016209280135No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14490.20914734567625032No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14470.20885866749036178No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA14250.2056832074455878No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC13830.19962096554192837No Hit
TCACTGGACTCCAGGCTGACGATGAGGCTGATTATTACTGCCTGTCCTAT13820.1994766264489841No Hit
GTCATAGGACAGGCAGTAATAATCAGCCTCATCGTCAGCCTGGAGTCCAG13510.19500211456771163No Hit
AGTCTGTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCA12970.18720780354872094No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGCACGGTGACCTTGGTCCCAGTGCCGAAGACATAACCATAGA12370.17854745797206462No Hit
GATGTACACTGGTACCTGCAACTTCCAGGAACTGCCCCCAAACTCCTCATCTATGGTAATAACAATCGGCCCTCA12350.17825877978617605No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12140.17522765883434635No Hit
GCCTTGGGCTGACCTAGCACGGTGACCTTGGTCCCAGTGCCGAAGACATA11430.16497958323530304No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG11350.16382487049174888No Hit
GTGCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG11320.16339185321291605No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT11190.1615154450046405No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGTCCAGTGATGGCCAGGGAGGCAGAGGTGCCAGACTTGGAACCAGA10890.15718527221631234No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10720.1547315076362597No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT10640.15357679489270554No Hit
GTTCCAATATCGGGACAGGTTATGATGTACACTGGTACCTGCAACTTCCA10150.14650417933843618No Hit
GCACAGGGTCCTGGGCCCAGTCTGTACTGACGCAGCCGCCCTCAGTGTCT9950.14361739747955077No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9850.14217400655010803No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG9760.14087495471360958No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC9520.13741081648294706No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGTTCCAATATCGGGACAGGTTATGATGTACACTGGTACCT9480.13683346011116995No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC9280.1339466782522845No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAG8940.12903914909217926No Hit
ACCATAGAGGCTGCTGTCATAGGACAGGCAGTAATAATCAGCCTCATCGTCAGCCTGGAGTCCAGTGATGGCCAG8940.12903914909217926No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8840.12759575816273655No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAG8830.12745141906979227No Hit
GACCTAGCACGGTGACCTTGGTCCCAGTGCCGAAGACATAACCATAGAGG8270.11936842986491304No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC8270.11936842986491304No Hit
CTCCTCATCTATGGTAATAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGTTCCAAGTCTGGCACCT8230.11879107349313596No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCT8210.11850239530724742No Hit
GGCCATCACTGGACTCCAGGCTGACGATGAGGCTGATTATTACTGCCTGT8130.11734768256369324No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8070.1164816480060276No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGTTCCAATATCGGGACAGGTTATGAT8070.1164816480060276No Hit
GTCTGGCACCTCTGCCTCCCTGGCCATCACTGGACTCCAGGCTGACGATG8070.1164816480060276No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8060.11633730891308332No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA7950.11474957889069633No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC7920.11431656161186352No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCAT7890.1138835443330307No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT7690.11099676247414526No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG7500.10825431970820408No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT7460.10767696333642701No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACT7370.10637791149992855No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTG7340.10594489422109574No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG7340.10594489422109574No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG7190.10377980782693165No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC7180.10363546873398738No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7060.1019033996186561No Hit
ACCTAGCACGGTGACCTTGGTCCCAGTGCCGAAGACATAACCATAGAGGC7050.10175906052571183No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGT6960.1004600086892134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)