FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690204
Sequences flagged as poor quality0
Sequence length18-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT40940.5931579648915393No Hit
ATATTATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA34520.5001419870067401No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27770.40234481399702116No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT25270.3661236388082364No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19850.28759613099895104No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18710.27107927511286517No Hit
CTATAATATTGCTGACAGTAATAAATCGCCGCATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGAC18550.26876111990078294No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT18280.2648492329803942No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17650.2557214968328204No Hit
TTATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCT17240.24978122410185974No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16340.23674160103389721No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16050.23253994471199818No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15470.22413663206820011No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15300.22167359215536278No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT14770.21399470301534038No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGTACATCATGATGACCCAG14250.2064606985760732No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC13640.1976227318300097No Hit
GTACTATAATATTGCTGACAGTAATAAATCGCCGCATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAA13010.1884949956824359No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12860.18632172517110884No Hit
ATTTTATACACCAACAATAAAAACTACATCGCTTGGTACCAGCAGAAACCGGGACAGCCTCCAAAATTGTTCATT12820.18574218636808829No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12580.18226495354996494No Hit
ATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA11950.17313721740239119No Hit
ATTCAGTGGCAGCGGGTCTGGGTCAGATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGATGCGGCGATTTA11620.1683560222774716No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11450.16589298236463423No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11270.1632850577510417No Hit
GTACCAGCAGAAACCGGGACAGCCTCCAAAATTGTTCATTTACTGGACATCTATCCGGCAATCCGGGGTCCCTGA11170.16183621074349033No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC10830.1569101309178156No Hit
CACCATCAACAGCCTGCAGCCTGAAGATGCGGCGATTTATTACTGTCAGCAATATTATAGTACTCCTCTCACTT10490.15198405109214086No Hit
GTATTTTATACACCAACAATAAAAACTACATCGCTTGGTACCAGCAGAAACCGGGACAGCCTCCAAAATTGTTCA10350.14995566528156892No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10320.14952101117930353No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10150.14705797126646616No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9960.1443051619521185No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAACCAGAGTATTTTATACACCAACAATAAAAACTACA9690.14039327503172974No Hit
GGATCTCTGGTGCCTACGGGTACATCATGATGACCCAGTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGG9160.13271438589170736No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9110.1319899623879317No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA9090.1317001929864214No Hit
GAGTACTATAATATTGCTGACAGTAATAAATCGCCGCATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAA9010.1305411153803803No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGATGCGGCGATTTATTACTGTCAGCAATATTATAGTAC8960.1298166918766046No Hit
GTCCCTGATCGATTCAGTGGCAGCGGGTCTGGGTCAGATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGA8930.12938203777433918No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8880.1286576142705635No Hit
ATTTTGGAGGCTGTCCCGGTTTCTGCTGGTACCAAGCGATGTAGTTTTTATTGTTGGTGTATAAAATACTCTGGT8870.12851272956980836No Hit
ATTTATTACTGTCAGCAATATTATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA8690.12590480495621584No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8670.12561503555470557No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGG8520.12344176504337848No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8520.12344176504337848No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8510.12329688034262334No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8480.12286222624035793No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8430.12213780273658223No Hit
CCATCAACAGCCTGCAGCCTGAAGATGCGGCGATTTATTACTGTCAGCAA8360.12112360983129625No Hit
CAATAAAAACTACATCGCTTGGTACCAGCAGAAACCGGGACAGCCTCCAAAATTGTTCATTTACTGGACATCTA8340.12083384042978597No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT8230.11924010872147944No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8170.11837080051694861No Hit
GCCTGAAGATGCGGCGATTTATTACTGTCAGCAATATTATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGG8140.1179361464146832No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8110.11750149231241779No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC7860.1138793747935393No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7720.11185098898296734No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7500.1086635255663543No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7460.10808398676333375No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7370.10678002445653749No Hit
CTCCAAAATTGTTCATTTACTGGACATCTATCCGGCAATCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGTC7260.10518629274823096No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG7230.10475163864596554No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7220.1046067539452104No Hit
AACCGGGACAGCCTCCAAAATTGTTCATTTACTGGACATCTATCCGGCAATCCGGGGTCCCTGATCGATTCAG7200.10431698454370013No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT7180.10402721514218985No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG7140.10344767633916928No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA7130.10330279163841415No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7020.10170905993010762No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7010.10156417522935249No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7000.10141929052859736No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7000.10141929052859736No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6990.10127440582784221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA855.37125E-662.84795470
TGCGGCA550.004594444858.277270
TATTCTA600.006482847553.42076570
AAAACCG1601.4824764E-953.4207670
CGTTATG200.00863941549.6898427
GGTAGTT858.710231E-846.91691669
TCTCGCG2301.4551915E-1146.4528470
AAAGGGG7400.044.85704868
AATGGTA4050.044.84705770
AAGGGGG9050.044.69511869
TGTTAGA9200.044.13019670
TCGTATG8200.043.48840740
CGTATGC8350.043.2516441
TCTCGTT359.173935E-442.63285462
ATGCCGT8400.042.61068344
ATCTCGT7250.041.65881337
CGTCTTC8700.041.3137948
GATCTCG7400.041.15003636
CTTGAAA9050.041.0399957
CTTATAC8600.040.970681
Produced by FastQC (version 0.11.5)