FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004685689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690204
Sequences flagged as poor quality0
Sequence length18-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74821.0840273310499504No Hit
ATATTATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT32950.4773950889881832No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA25550.3701804104293803No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24820.35960382727425516No Hit
CTATAATATTGCTGACAGTAATAAATCGCCGCATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGAC22150.320919612172633No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19050.27600535493853995No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC18020.2610822307607606No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT18010.26093734606000546No Hit
TTATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT16880.24456537487467472No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA16620.24079837265504112No Hit
GTACTATAATATTGCTGACAGTAATAAATCGCCGCATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAAT16000.23181552120822252No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC15920.23065644360218138No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15390.22297755446215903No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15030.217761705234974No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13670.19805738593227507No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA13620.1973329624284994No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC13230.19168245909904896No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12690.18385868525827145No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC12210.1769042196220248No Hit
ATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA11680.16922533048200242No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11650.168790676379737No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11270.1632850577510417No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA11220.16256063424726602No Hit
ATTCAGTGGCAGCGGGTCTGGGTCAGATTTCACTCTCACCATCAACAGCC10570.15314312869818197No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA10440.15125962758836517No Hit
GTTGATGGTGAGAGTGAAATCTGACCCAGACCCGCTGCCACTGAATCGATCAGGGACCCCGGATTGCCGGATA10330.14966589588005866No Hit
GAGTACTATAATATTGCTGACAGTAATAAATCGCCGCATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAAT10320.14952101117930353No Hit
ATTTTATACACCAACAATAAAAACTACATCGCTTGGTACCAGCAGAAACC10290.1490863570770381No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT9830.14242166084230168No Hit
CACCATCAACAGCCTGCAGCCTGAAGATGCGGCGATTTATTACTGTCAGCAATATTATAGTACTCCTCTCACTTT9700.1405381597324849No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT9590.13894442802417836No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9430.13662627281209613No Hit
GTACCAGCAGAAACCGGGACAGCCTCCAAAATTGTTCATTTACTGGACATCTATCCGGCAATCCGGGGTCCCT9040.13097576948264572No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9000.13039623067962514No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8830.12793319076678777No Hit
GAGTGAAATCTGACCCAGACCCGCTGCCACTGAATCGATCAGGGACCCCG8810.1276434213652775No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG8520.12344176504337848No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGATGCGGCGATTTATTACTGTCAGCAATATTATAGTACT8470.12271734153960279No Hit
GTAATAAATCGCCGCATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGACCCAGACCCGCTGCCA8470.12271734153960279No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8460.12257245683884764No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8430.12213780273658223No Hit
TCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGACCCAGACCCGCTGCCACTGAATCGATCAGGGACC8400.12170314863431682No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT8360.12112360983129625No Hit
ATTTATTACTGTCAGCAATATTATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA8180.11851568521770374No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT8100.11735660761166265No Hit
ATTTTGGAGGCTGTCCCGGTTTCTGCTGGTACCAAGCGATGTAGTTTTTA7960.11532822180109069No Hit
GTATTTTATACACCAACAATAAAAACTACATCGCTTGGTACCAGCAGAAA7910.11460379829731501No Hit
GGATCTCTGGTGCCTACGGGTACATCATGATGACCCAGTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGG7840.11358960539202903No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCA7720.11185098898296734No Hit
GCCTGAAGATGCGGCGATTTATTACTGTCAGCAATATTATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGGT7700.11156121958145707No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7630.11054702667617111No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATA7550.10938794907013No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAACCAGAGTATTTTATACACCAACAATAAAAACTACAT7510.10880841026710943No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7400.1072146785588029No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT7340.10634537035427206No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA7290.10562094685049637No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7220.1046067539452104No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC7140.10344767633916928No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAG7030.10185394463086275No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGGAGTA7010.10156417522935249No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7010.10156417522935249No Hit
CCATCAACAGCCTGCAGCCTGAAGATGCGGCGATTTATTACTGTCAGCAATATTATAGTACTCCTCTCACTTTCG6950.10069486702482164No Hit
GTCCCTGATCGATTCAGTGGCAGCGGGTCTGGGTCAGATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGAT6910.1001153282218011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)