FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018986
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCTGCGGCGGCCACAGAGCTCAGCTTCAGGGAGA53300.5230690117430465No Hit
GTCTATTACTGTGCGAGAGATCGCCAGTTTGGAGACGACACGAACTGGTT34700.34053460989650497No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAGACA33800.33170230012973684No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31260.3067755592324134No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27480.2696798582119872No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATGATCATTACTACCATCCCACAC27150.2664413446308389No Hit
GTCTCTGGTTACTCCATCAGCAGTGGTTATTACTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGGCCTGGAGTGG26930.26428233557674No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26300.2580997187400023No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA25500.2502487767250973No Hit
GGTTATTACTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGGCCTGGAGTGGATTGGGAGTATCTATCATAGTGGG24950.24485125408985012No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23870.23425248236972834No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATGATCATTACTACCATCCCACACCTGACAGTAAT23520.23081769523820742No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACA22440.22021892351808564No Hit
GGATTACCTGTGGGGGAGACAACATTGGAGGTAAAGGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC22250.21835432478954567No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22140.21727482026249623No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC20960.20569468079051134No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC20960.20569468079051134No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19870.19499777229520326No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATG19350.189894659985515No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19280.18920770255921082No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT19080.18724496705548457No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTG18720.18371204314877732No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18440.18096421344356056No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGGGGAAGACCGATGGGCCCTTGGTGG17440.1711505359249293No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACT17100.16781388556859467No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC17010.16693065459191786No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC16710.16398655133632847No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGC16670.1635940042355832No Hit
CCTTTACCTCCAATGTTGTCTCCCCCACAGGTAATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG16460.16153313195667066No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACC16410.1610424480807391No Hit
CAGTAATAGACGGCCGTGTCTGCGGCGGCCACAGAGCTCAGCTTCAGGGA16230.15927598612738544No Hit
GTCCCTCACCTGCGCTGTCTCTGGTTACTCCATCAGCAGTGGTTATTACT16180.1587853022514539No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT15860.15564492544549188No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACCTTTACCT15500.15211200153878462No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTG15380.15093436023654888No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15210.14926603505838157No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAGACAACATTGGAGGTAAAGGTGTGCACTGGTACCAGCAGA15190.14906976150800894No Hit
CTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGT15180.14897162473282263No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC15150.1486772144072637No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15100.14818653053133213No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG15010.14730329955465532No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14910.14632193180279218No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT14840.145634974376488No Hit
CTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC14800.14524242727574274No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14610.14337782854720282No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14500.14229832402015336No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14470.14200391369459442No Hit
GGATTGGGAGTATCTATCATAGTGGGAGGACCTATAACAACCCGTCCCTCAAGAGTCGAGTCACCCTATCATT14200.139354220764564No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14170.13905981043900506No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCC14160.13896167366381873No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC13940.13680266460971985No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13880.13621384395860198No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG13850.13591943363304304No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACT13730.1347417923308073No Hit
GGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTC13620.13366228780375786No Hit
CCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAC13620.13366228780375786No Hit
ATAACAACCCGTCCCTCAAGAGTCGAGTCACCCTATCATTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTG13620.13366228780375786No Hit
GTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTCCTGTCCCAGGTGC13560.13307346715263996No Hit
GTTATAGGTCCTCCCACTATGATAGATACTCCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGCCGGATCCAGCC13510.13258278327670842No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG13490.1323865097263358No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCAT13420.1316995523000316No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13290.13042377422260953No Hit
CATTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG13140.12895172259481485No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13000.12757780774220645No Hit
GTAGTAATGATCATGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTA12990.12747967096702018No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTA12930.12689085031590228No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCC12870.1263020296647844No Hit
GGGTTCCCTGGCCCCAGGAGTCGAACCAGTTCGTGTCGTCTCCAAACTGGCGATCTCTCGCACAGTAATAGACGG12790.1255169354632939No Hit
CTATTACTGTCAGGTGTGGGATGGTAGTAATGATCATGTGGTATTCGGCG12760.12522252513773496No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC12730.12492811481217601No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12220.11992313927767409No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12180.11953059217692882No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12110.11884363475062464No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT12080.1185492244250657No Hit
GCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA12060.11835295087469308No Hit
GTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC12020.11796040377394783No Hit
CCTTGGTCCCTCCGCCGAATACCACATGATCATTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCATCCC12020.11796040377394783No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11950.11727344634764364No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTCTGGTTACTCCATCAGCAGTGGTTATT11920.1169790360220847No Hit
CTATTACTGTGCGAGAGATCGCCAGTTTGGAGACGACACGAACTGGTTCGACTCCTGGGGCCAGGGAACCCTGGT11860.11639021537096683No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC11680.1146237534176132No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC11670.11452561664242687No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA11670.11452561664242687No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCC11540.11324983856500481No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11470.11256288113870062No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11430.11217033403795537No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT11330.11118896628609225No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAG11130.10922623078236599No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11080.10873554690643443No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11080.10873554690643443No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTG10950.10745976882901237No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC10900.1069690849530808No Hit
CCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACT10890.1068709481778945No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAG10850.10647840107714925No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATGGTAGTA10790.10588958042603137No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAAC10790.10588958042603137No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT10760.10559517010047244No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGA10760.10559517010047244No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTC10740.1053988965500998No Hit
GTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCG10710.10510448622454087No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC10540.10343616104637354No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10470.10274920362006935No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC10400.10206224619376517No Hit
ATCATTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGGCCGCCGCAGACACGGCCGTCTATT10380.10186597264339255No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTG10380.10186597264339255No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10370.10176783586820623No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10360.10166969909301993No Hit
GGGGAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCGAACC10330.10137528876746099No Hit
GTAATAACCACTGCTGATGGAGTAACCAGAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTC10250.10059019456597049No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG10210.10019764746522523No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10190.10000137391485261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)