FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081018
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40500.37464686064431857No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA36100.3339444856607383No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27280.25235472489819777No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT26880.2486545089905996No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25640.23718383967704515No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC24370.22543565417042083No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGTCCAGTCAGAGTGTTAGCA22950.2122998876984472No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21450.1984240780449539No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA20580.1903761084459278No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20220.1870459141290894No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20210.18695340873139948No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGTCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA19940.1844557629937707No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19550.18084805248386243No Hit
CAATAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCTTCAGGAAGAACTGGTTCTTAGACGTGTCTA18930.1751127178270852No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA18580.1718750289079368No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC18520.17131999652179705No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17640.163179521525081No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA17380.16077438118514217No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCACTCTTTTCGGCCCTGGGACCCAAGTG17020.15744418686830375No Hit
GAGTGAAGTCTGTCCCAGAGCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATA16650.15402148715377542No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC16630.1538364763583955No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16420.15189386300690644No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT16420.15189386300690644No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA16340.1511538198253868No Hit
GTATTATTGTGCGAGGGCCTATTACTATGATAGTAGTGGTTATTCCCCCGTGGGCTATTGGGGCCAGGGAACC16020.14819364709930824No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15720.14541848516860958No Hit
GTTTATTACTGTCAGCAGCGTAGCACTCTTTTCGGCCCTGGGACCCAAGTGGATATCAAACGAACTGTGGCTGCA15540.14375338801019039No Hit
CTCCTGCAGGTCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT15130.13996066670490223No Hit
ATAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCTTCAGGAAGAACTGGTTCTTAGACGTGTCTACT15120.13986816130721227No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA15080.13949813971645245No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTGGGTCCCAGGGCCGAAAAGAGTGCTACGCTGCTGACAGTAATAAA15050.1392206235233826No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14960.138388074944173No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA14770.13663047238806383No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGAGCCACTGCCA13810.12774995420982815No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13810.12774995420982815No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13770.12737993261906833No Hit
GTCCCAGAGCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCCTG13530.1251598030745094No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13500.12488228688143953No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC13450.12441975989298974No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13350.1234947059160902No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG13230.12238464114381073No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTGGGTCCCAGGGCCGAAAAGAGTGCTACGCT12980.12007200620156185No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12970.1199795008038719No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTCTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCA12730.11775937125931299No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12640.11692682268010339No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12570.1162792848962737No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12430.11498420932861432No Hit
GTCTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGTCCAGTCA12350.11424416614709469No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12200.11285658518174536No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC12010.11109898262563621No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG11840.10952639086490695No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT11710.10832382069493754No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11650.10776878830879781No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGCTCTGGGACAGACTTCACT11600.10730626132034804No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11510.10647371274113844No Hit
GTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC11360.10508613177578911No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11290.10443859399195943No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11190.10351354001505989No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTATCACTGGAGCTGGATCCG11080.10249598064047036No Hit
CTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT10910.10092338887974113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA2100.072.1866970
TTGCAAT1052.3608918E-672.1866970
GGCGCAA1205.216194E-663.16335370
CATACAT800.005070116456.8470270
AATAACA1102.5805164E-455.12438270
GACGGAA850.006444522653.50307570
GTAGTTT1451.6008562E-552.2731270
GCTTGCA900.00807871550.53068570
CGATTAA1305.8843585E-446.64370370
CTTCCAT3155.5661076E-1043.31201670
GAGCAAA20900.042.06860770
GGTATCA9150.038.8184971
GTATCAA20800.038.5438841
GAGCCAT12000.037.8980170
ACCAGGT3304.0467057E-836.74958870
AAGGACA21450.035.33614370
TCTGAGT2601.2166611E-534.9827870
GACAGGT2150.034.614231
CGTCACA10750.033.84380370
GCCGTTA502.0940849E-433.81501415
Produced by FastQC (version 0.11.5)