FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496663
Sequences flagged as poor quality0
Sequence length18-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA83020.5547006908034742No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG42980.28717219574480024No Hit
CTATATTACTGTGCGAAAGATCTGGATTATGATCTTTGGAGGGGGGCTTTTGATATTTGGGGCCAAGGGACAA41470.2770830841679122No Hit
GGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGGCTTTTT41360.2763481157748939No Hit
ATATTACTGTGCGAAAGATCTGGATTATGATCTTTGGAGGGGGGCTTTTGATATTTGGGGCCAAGGGACAATGG38250.2555685548450119No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAATACGAAAGTGTTCCTACCTGCATATGAGCAG38230.25543492422809944No Hit
GATTATTACTGCTGCTCATATGCAGGTAGGAACACTTTCGTATTCGGCGGAGGGACCCGGCTGACCGTCCTAGG37060.2476175331387226No Hit
CAGTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGGATTGTCTC37010.24728345659644158No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34990.23378676428828668No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA33770.22563529665662876No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT31760.21220541965693013No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGACCTGCGTCCGCCAGGCTCCAGGGAAGGGG29870.19957732635870598No Hit
GTAATAATCAGCCCCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG29420.19657063747817644No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCC29320.19590248439361435No Hit
GACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAATACGAAAGTGTTCCTACCTGCATATGAGCAGCAGTAATAA29170.19490025476677114No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG29160.19483343945831494No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAA28890.1930294261299972No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT28870.19289579551308478No Hit
GTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAA27640.1846775125729707No Hit
GCCCTATATTACTGTGCGAAAGATCTGGATTATGATCTTTGGAGGGGGGC26980.18026770221486066No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG26860.1794659185133861No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25920.17318527951850216No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT25850.17271757235930868No Hit
ATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGGATTGTCTCTGGAGA25350.16937680693649806No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25290.1689759150857608No Hit
GTGTTGGTACCAGGAGACAAGGTTAAAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA24740.16530107312066913No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24460.16343024448389518No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCGGGTCCCTC24380.16289572201624547No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24300.1623611995485958No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTT24230.1618934923894023No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA24200.16169304646403365No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC24030.16055718622027804No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC23230.15521196154378106No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT23070.15414291660848167No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22780.1522052726632515No Hit
GTATGTGCTACCGCTACCGCTAATAGTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACGCAGGTCA21980.14686004798675453No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG21740.14525648058380544No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC21710.1450560346584368No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTTTAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGC21680.14485558873306817No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21150.14131437738488892No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG21090.14091348553415164No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGT21010.14037896306650194No Hit
GGACAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC20800.13897584158892148No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20780.13884221097200908No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTTTAACCTTGTCTCCTGGTACCAACACCA20730.138508134429728No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20610.13770635072825346No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20410.1363700445591292No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20360.13603596801684814No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20350.13596915270839194No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT20320.1357687067830233No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGG20310.1357018914745671No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19840.13256157197712512No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACGCAGGTCATGGCATAGTTGC19630.13115845049954464No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG19580.1308243739572636No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG19570.1307575586488074No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC19440.12988895963887662No Hit
GAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTG19220.12841902285283996No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGG19200.12828539223592753No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT19160.12801813100210266No Hit
GGGCTGGAGTGGGTCTCAACTATTAGCGGTAGCGGTAGCACATACTACGC19130.12781768507673405No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC19020.1270827166837157No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18870.12608048705687253No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18780.12547914928076662No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18750.12527870335539798No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18540.12387558187781753No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18450.1232742441017116No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC18290.12220519916641222No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC18080.12080207768883175No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG17820.11906487966897024No Hit
AGCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATCCCAAGAACACACTGTATC17810.11899806436051404No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG17550.11726086634065251No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17530.11712723572374008No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17140.11452143869394779No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA16980.1134523937586484No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16750.11191564166415553No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC16720.11171519573878688No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT16660.11131430388804961No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16600.11091341203731235No Hit
CCATTGTCCCTTGGCCCCAAATATCAAAAGCCCCCCTCCAAAGATCATAA16580.11077978142039992No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT16450.10991118241046917No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT16280.10877532216671355No Hit
GTTTTAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAA16200.10824079969906386No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG16150.10790672315678278No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT16100.10757264661450172No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15830.10576863328618401No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCC15710.10496684958470945No Hit
GTGTGAGGTGCAGCTGGTGCAGTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC15470.10336328218176036No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAG15270.1020269760126361No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15030.10042340860968701No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAG14970.10002251675894974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)