FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496663
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGGATTGTCTCTGGA70480.4709142939993839No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42890.28657085796869436No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT42510.2840318762473583No Hit
GGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGGCTTTTT38520.2573725681733296No Hit
ATATTACTGTGCGAAAGATCTGGATTATGATCTTTGGAGGGGGGCTTTTGATATTTGGGGCCAAGGGACAATGGT36400.2432077227806126No Hit
CTATATTACTGTGCGAAAGATCTGGATTATGATCTTTGGAGGGGGGCTTTTGATATTTGGGGCCAAGGGACAATG35990.2404682951339079No Hit
GATTATTACTGCTGCTCATATGCAGGTAGGAACACTTTCGTATTCGGCGGAGGGACCCGGCTGACCGTCCTAGGT35130.23472217860667366No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAATACGAAAGTGTTCCTACCTGCATATGAGCA34920.23331905712909318No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34430.23004510701473876No Hit
CAGTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGGATTGTCTCTG33410.22322994555220516No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGACCTGCGTCCGCCAGGCTCCAGGGAAGGG29960.2001786641348119No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCT28380.18962184539873037No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC28350.18942139947336173No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27740.18534566565753277No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG27550.18407617479686475No Hit
GTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAA27390.18300712986156537No Hit
GACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAATACGAAAGTGTTCCTACCTGCATATGAGCAGCAGTAATAAT27170.1815371930755287No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG27020.18053496344868553No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG26500.17706056740896248No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26270.1755238153144696No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC26080.17425432445380154No Hit
GCCCTATATTACTGTGCGAAAGATCTGGATTATGATCTTTGGAGGGGGGC26010.17378661729460806No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCA25850.17271757235930868No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG25270.16884228446884836No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT24810.1657687802798626No Hit
GTAATAATCAGCCCCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGA24730.1652342578122129No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24380.16289572201624547No Hit
GTGTTGGTACCAGGAGACAAGGTTAAAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATC24270.16216075362322716No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA23750.1586863575835041No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC23250.1553455921606935No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCGGGTCCCTC22560.15073533587721483No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22380.149532660325003No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA21470.1434524672554877No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA21090.14091348553415164No Hit
GGGCTGGAGTGGGTCTCAACTATTAGCGGTAGCGGTAGCACATACTACGC21080.14084667022569544No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTTTAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGC21060.140713039608783No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20790.13890902628046528No Hit
GTATGTGCTACCGCTACCGCTAATAGTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACGCAGGTCAT20760.13870858035509664No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT20700.13830768850435934No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG20600.13763953541979726No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19920.1330960944447748No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACA19830.13249475666866892No Hit
ATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGGATTGTCTCTGGAGAT19810.13236112605175648No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT19200.12828539223592753No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19080.12748360853445298No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTTTAACCTTGTCTCCTGGTACCAACACCA18700.12494462681311691No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG18550.12394239718627373No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18390.12287335225097433No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18160.12133660015648144No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACGCAGGTCATGGCATAGTTGCTAAAGGTGAATCCAGAGGCTGCACA18120.1210693389226566No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG18110.1210025236142004No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17910.11966621744507613No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17900.11959940213661993No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17900.11959940213661993No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG17870.1193989562112513No Hit
GAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGT17830.11913169497742644No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGGC17610.11766175819138977No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC17570.11739449695756493No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG17390.1161918214053531No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT17340.11585774486307206No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT17340.11585774486307206No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC17300.1155904836292472No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC17270.11539003770387857No Hit
GGACAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC17170.11472188461931643No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA17110.11432099276857917No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT17080.11412054684321053No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT17080.11412054684321053No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16880.11278424067408628No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG16850.11258379474871763No Hit
AGCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATCCCAAGAACACACTGTATCT16770.11204927228106794No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT16730.1117820110472431No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC16700.11158156512187445No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16610.11098022734576855No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGA16590.11084659672885612No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG16480.11011162833583779No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGG16450.10991118241046917No Hit
GTGTGAGGTGCAGCTGGTGCAGTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC16340.10917621401745083No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAATAC16110.10763946192295794No Hit
GTTTTAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGC15930.1064367863707461No Hit
CCCCTGAGGGCCGCTTACTGCCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGA15810.10563500266927157No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG15640.10449914242551596No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGT15270.1020269760126361No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT15180.1014256382365302No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGT15170.101358822928074No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGAT15160.10129200761961776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)