FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200335
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTC53040.4418766427705599No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGAAGATACAGCGTGTTCTTGGAATTGTCTCTGGA46100.3840594500701887No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT43250.3603160784281055No Hit
GTGTATTACTGTGTGAAGGGGAGTTACGATTTTTGGAGTGGTTATTCAAACTTTGACTACTGGGGCCAGGGAACC39310.3274919085088746No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC35560.2962506300324493No Hit
GTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC34160.2845872194012505No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGA32800.2732570490738002No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31710.2641762507966526No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30070.2505133983429626No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGAAGATACAGCGTGTTCTTGGAATTGTCTCTG29330.2443484527236147No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAAGACCATTCAGGCTGTCATCCCAT28310.23585082497802698No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27370.22801967783993635No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT26080.21727267804404604No Hit
GTATTACTGTGTGAAGGGGAGTTACGATTTTTGGAGTGGTTATTCAAACTTTGACTACTGGGGCCAGGGAACC25520.2126073137915665No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23150.1928628257944657No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG22880.19061345374416308No Hit
AGTACATACTACGCAGACTCCGTGAAGGGCAGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTT22870.19053014366822593No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCA22780.18978035298479176No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22030.18353209728950667No Hit
GTATGTACTACCCCCATTTCTACTAATAGCTGAAACATATTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGT21460.17878342296109004No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC21290.17736715167015873No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA21110.17586757030329034No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21100.1757842602273532No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21030.17520108969579326No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20800.17328495794923918No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA20690.1723685471139307No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAAGACCATTC20410.17003586498769094No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG20020.1667867720261427No Hit
CTCCTGTTCAGCCTCTGGATTCACCTTCAGTAACTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGG20010.16670346195020558No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19800.16495395035552574No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19710.16420415967209154No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC19550.16287119845709738No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG19090.15903893496398921No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG18940.1577892838249322No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCA18240.1519575785093328No Hit
GTCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGTGAAGGGGAGTTAC18110.15087454752215007No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17830.1485418653959103No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17750.14787538478841325No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG17710.14754214448466468No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17670.14720890418091617No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCTTGTGGTATTCGGCGGAGG17430.14520946235842494No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17270.14387650114343079No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT17230.14354326083968227No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT16950.1412105787134425No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16950.1412105787134425No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16790.13987761749844835No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG16740.1394610671187627No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA16610.13837803613157992No Hit
CCCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGTTTA16490.1373783152203343No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16460.13712838499252292No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG15950.13287957111972906No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT15910.13254633081598055No Hit
GCATGGGATGACAGCCTGAATGGTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG15640.1302969587656779No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT15570.12971378823411797No Hit
GCACTGAAGACAGAAGACTCATCATGGAGTTCTGGCTGAGCTGGGTTCTC15330.12771434641162674No Hit
CCACAAGACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACT15210.12671462550038115No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14730.1227157418553987No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTTTGAATAACCACTCCAAAAATCGTAACTCCCCTTCACACAGTAATACA14410.1200498194254104No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC14360.11963326904572474No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14290.11905009851416479No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT14240.11863354813447913No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG14220.11846692798260486No Hit
CATCTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC14220.11846692798260486No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG14160.11796706752698206No Hit
GTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGTTTACAGAATTACTTCCGATGTTGGA14050.11705065669167358No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG13970.11638417608417649No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT13950.11621755593230222No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13840.11530114509699377No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC13820.1151345249451195No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG13740.11446804433762241No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTTTGAATAAC13700.11413480403387388No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG13630.11355163350231395No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATG13540.11280184281887973No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG13350.11121895137607418No Hit
GATTATTAGTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGTTTACAGAATTACTTCC13350.11121895137607418No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC13180.1098026800851429No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG13130.10938612970545723No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13020.10846971887014875No Hit
GTTCCTGGGAGGTGCTGGTACCAGTTTACAGAATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGT12880.10730337780702887No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGGCCCGGGATTCCCAGGTGTTTCCATTCAGT12830.1068868274273432No Hit
CTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG12760.10630365689578325No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT12710.10588710651609759No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT12700.10580379644016046No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC12690.10572048636422332No Hit
GATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGTTTACAG12590.10488738560485197No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTCTGTAAACTGGTACCAGCACCTCCCAGGAACGGCCCC12570.1047207654529777No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC12400.10330449416204643No Hit
GTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGTTTACAGAATTACTT12330.10272132363048649No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC12300.1024713934026751No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTTTC12280.10230477325080083No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12240.10197153294705227No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC12120.10097181203580667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)