FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718592
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39940.5558091378696117No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29800.41469985749910937No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTGAGGGAGAACTGGTTCTTGGACGTGTCTACT27690.38533688101175634No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA26430.36780259173494834No Hit
GTATTACTGTCAACAACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGT22710.3160346900605629No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19860.27637379764873526No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT19530.2717814837905237No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18870.26259685607410044No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC18040.25104649091556824No Hit
GTCTCCATCCTTCCTGTCTGCATCTATAGGAGACAGAATCACCATCACTT17750.2470108211613823No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGTAGTAGATCTGGGACAGAATTC17330.24116605806911293No Hit
GTCTATTACTGTGCGAGAGTAAACGAGGAGGGGCTGCCCCGTTCCTACTACTACTACATGGACTTCTGGGGCAAA16550.23031149804061277No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16050.22335344674029212No Hit
GTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGCGAATTCTGTCCCAGATCTACTACCGCTGAATCTTGATGGGAC15960.2221009975062344No Hit
CAGTAATACGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGCGAATTCTGTCCCAGATCTACTACC15550.21639539543997147No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC15510.21583875133594585No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA15250.21222056465977912No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14910.2074890897755611No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA14560.20261845386533667No Hit
CACCTGTACTGTCTCTGATGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGG14080.19593872461702885No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13590.18911983434271465No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13590.18911983434271465No Hit
CCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAACTGCTAATGGCCTGACTG13400.18647577484859282No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC13400.18647577484859282No Hit
GTAGTAGATCTGGGACAGAATTCGCTCTCACAATCAGCAGCCTGCAGCCT13210.18383171535447096No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13080.18202262201638758No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12960.18035268970431065No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA12580.175064570716067No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12340.17172470609191307No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT12220.17005477377983613No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGT12140.16894148557178484No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT11830.16462749376558602No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11320.157530281439259No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA11270.15683447630922692No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA11180.15558202707516922No Hit
GTCTCTGATGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATT11100.1544687388671179No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11070.15405125578909867No Hit
GTGGATGCACCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAACTGCTAATG10930.1521030014250089No Hit
GCCTGAAGACTTTGCAACGTATTACTGTCAACAACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGGT10520.146397399358746No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGTAGTAGA10300.1433358567866049No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA10200.1419442465265408No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10110.14069179729248307No Hit
GTAATACGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGCGAATTCTGTCCCAGATCTACTACC10080.14027431421446385No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC9920.13804773779836124No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG9880.13749109369433557No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9680.13470787317420735No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC9680.13470787317420735No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9550.13289877983612397No Hit
ACGTATTACTGTCAACAACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA9290.12928059315995724No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9210.12816730495190595No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9120.12691485571784822No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT9050.12594072853580335No Hit
ACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT8960.12468827930174563No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8780.1221833808336302No Hit
CACCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCG8770.12204421980762378No Hit
GGCCATTAGCAGTTCTTTAGCCTGGTATCAGCAAACACCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATC8750.12176589775561097No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTT8720.12134841467759173No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8680.12079177057356609No Hit
ACCTACTACAATCCCTCCCTCAAAGGTCGAGTCTCCATGTCAGTAGACACGTCCAAGAACCAGTTCTCCCTCAA8570.11926099928749555No Hit
ATCTACTACCGCTGAATCTTGATGGGACCCCACTTTGCAAAGTGGATGCA8560.11912183826148913No Hit
GGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAACTGCTAATGGCCTGACTGGCCCGGCAAGT8280.11522532953330959No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8180.11383371927324547No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8090.11258127003918775No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG8010.11146798183113643No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7980.1110504987531172No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7900.1099372105450659No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7800.10854560028500178No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7730.1075714731029569No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7680.10687566797292483No Hit
GCTAATGGCCTGACTGGCCCGGCAAGTGATGGTGATT7560.10520573566084787No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTT7530.10478825258282864No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7500.1043707695048094No Hit
GTGGGGTCCCATCAAGATTCAGCGGTAGTAGATCTGGGACAGAATTCGCTCTCACAATCAGCAGCCTGCAGCCT7400.10297915924474528No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7340.1021441930887068No Hit
CCATCCTTCCTGTCTGCATCTATAGGAGACAGAATCACCATCACTTGCCG7240.10075258282864269No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7230.10061342180263627No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7230.10061342180263627No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7230.10061342180263627No Hit
CAGTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTGAGGGAGAACTGGTTCTTGGACGTGTCTA7220.10047426077662985No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7220.10047426077662985No Hit
GGACTGGAGTGGATTGGCTATATCTTTTACAGTGGGAGTACCTACTACAA7220.10047426077662985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)