FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355353
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT44060.3250813625675377No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42120.3107677483282953No Hit
GTCTACAAACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCT35310.2605225354575524No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG34700.2560218629390277No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCATGAATAGCAACGGATACAACTATT33200.24495463543445878No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGACTAGAACCCAAATAGA32370.2388307695485973No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT31100.2294605169280623No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30530.22525497047632606No Hit
GGTCTAGTCAGAGCCTCATGAATAGCAACGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC29030.2141877429717572No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27530.20312051546718823No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26850.19810337233178368No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGG26130.1927911031295906No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26110.19264354009619636No Hit
GTTTGTAGACCTTGCATGCAGTAATAAATCCCAACATCTTCAGCCTCCACTCTGCTGATTATCAGTGTAAAA25920.19124169127895094No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC25300.18666723724372913No Hit
GTATGTGGTAGCACCATTACCAAAAATAATTGAGACCCACTCTAGCCCCTTCCCTGGAGCCTGGCGGACCCAGT25150.18556051449327224No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24960.18415866567602684No Hit
GATTTTACACTGATAATCAGCAGAGTGGAGGCTGAAGATGTTGGGATTTATTACTGCATGCAAGGTCTACAAAC24550.18113362349144466No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24410.1801006822576849No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24380.17987933770759354No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23880.1761902618727372No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTC23510.17346034575494357No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23250.17154202632081827No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22770.16800051351935621No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT21550.15899916848230683No Hit
GGACTGAACAGAGAGTACTCACCATGGAGTTTGGGCTGAGCTGCCTTTTT21300.15715463056487866No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21180.15626925236451317No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC20870.15398202534690222No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20870.15398202534690222No Hit
CTCCCTACCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA20770.15324421017993098No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20610.152063705912777No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT19740.14564471396012701No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19400.1431361423924247No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGATAATCAGCAGAGTGGAGGCTGAAGA19150.14129160447499653No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGGAGTTTG18480.1363482428562891No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18400.1357579907227121No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18400.1357579907227121No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACCCAGTCTCCACTCTCCCTACCCGTCACCC17610.12992925090363913No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17340.12793714995281671No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC17270.12742067933593684No Hit
GATAATCAGCAGAGTGGAGGCTGAAGATGTTGGGATTTATTACTGCATGCAAGGTCTACAAACTCCTCTCACTT17130.12638773810217707No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTG16850.12432185563465753No Hit
GTCAGGGACCCCGGAGGCCCGACTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA16700.12321513288420065No Hit
GTAATAAATCCCAACATCTTCAGCCTCCACTCTGCTGATTATCAGTGTAAAATCTGTGCCTGATCCACTGCCA16660.12292000681741215No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16660.12292000681741215No Hit
CTCTAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGGAATAGGTGC16590.12240353620053226No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16030.11827177126549321No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15870.11709126699833917No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGTAGGGAGAGTGGAGACTGGGTCATCACAA15860.11701748548164205No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGACAGGTTCAG15690.11576319969779089No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA15580.11495160301412252No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15420.11377109874696852No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG15330.11310706509669437No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15320.11303328357999724No Hit
CTCCAGGGGTGACGGGTAGGGAGAGTGGAGACTGGGTCATCACAATATCCCCACTGGATCCAGAGACCCAGAGCA14990.11059849352899208No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTT14810.1092704262284438No Hit
ACCACATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAGAAATATAGTATATCTC14750.10882773712826105No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14730.1086801740948668No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA14630.10794235892789554No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC14380.10609782101046739No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGATAATCAGCAGAGTGGAGGCTGAAGATGTTGGGATTTA14370.10602403949377026No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14310.10558135039358751No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCGTTGCTATTC13800.10181849304203407No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCC13770.1015971484919427No Hit
CCCTACCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGT13700.10108067787506282No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13640.10063798877488005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT750.050.2206939
TATTCTA1251.334105E-648.26926870
TCGCGTA851.8189894E-1244.270938
GGTATCA8600.043.9267231
TGTTAGA13900.041.96069770
ATCGCGT903.6379788E-1241.76136837
TCGTATG1400.041.6584440
CTTATAC3900.041.513161
CGAGACT3950.041.06803523
ACGAGAC4050.040.0332822
AGGTACG350.001509990438.5280273
TGGTATC2200.038.3077052
TATACAC4350.037.9744643
TCCGAGA13250.037.94753670
CGTATGG557.608638E-637.48064841
CGTATGC1650.037.48064841
TGTGCGT753.549212E-837.32565353
TTATACA4350.037.1981052
ATACACA4450.037.1211054
GTATCAA20900.036.795761
Produced by FastQC (version 0.11.5)